Source Jouranl of CSCD
Source Journal of Chinese Scientific and Technical Papers
Included as T2 Level in the High-Quality Science and Technology Journals in the Field of Environmental Science
Core Journal of RCCSE
Included in the CAS Content Collection
Included in the JST China
Indexed in World Journal Clout Index (WJCI) Report
BAO Meiling, HU Zhiquan, ZHANG Qiang, HONG Hui, DENG Jun, PEI Yunxia, LI Bingtang. CONSTRUCTION OF A SHORTCUT NITROGEN REMOVAL SYSTEM FOR ALGAL-BACTERIAL SYMBIOSIS AND ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN SLUDGE[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(8): 35-42. doi: 10.13205/j.hjgc.202408005
Citation: BAO Meiling, HU Zhiquan, ZHANG Qiang, HONG Hui, DENG Jun, PEI Yunxia, LI Bingtang. CONSTRUCTION OF A SHORTCUT NITROGEN REMOVAL SYSTEM FOR ALGAL-BACTERIAL SYMBIOSIS AND ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN SLUDGE[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(8): 35-42. doi: 10.13205/j.hjgc.202408005

CONSTRUCTION OF A SHORTCUT NITROGEN REMOVAL SYSTEM FOR ALGAL-BACTERIAL SYMBIOSIS AND ANALYSIS OF MICROBIAL COMMUNITY STRUCTURE IN SLUDGE

doi: 10.13205/j.hjgc.202408005
  • Received Date: 2023-04-10
    Available Online: 2024-12-02
  • The conventional biological nitrogen removal of wastewater with low C/N ratio and high ammonia nitrogen has some deficiencies, such as high energy consumption of aeration, and high amount of added carbon source. In this study, a shortcut nitrogen removal system for algal-bacterial symbiosis was constructed by combining shortcut nitrification functional sludge enriched with ammonia-oxidizing bacteria (AOB) with Chlorella. The effects of the film coating method and light intensity on nitrogen and phosphorus removal in the system were studied. The high-throughput sequencing technology was used to analyze the microbial community structure, and to analyze and determine the nitrogen conversion pathway in the system. The results demonstrated that the relative abundance of AOB in acclimated sludge was 19.31%, of which the main strain was Nitrosomonas, accounting for 94.41% of total AOB. The shortcut nitrification ability of acclimated sludge was excellent. An efficient nitrogen removal was achieved in the algal-bacterial symbiotic system. When first inoculating sludge and then inoculating microalgae, and the light intensity was set to 5000 lux, the best removal efficiency of TN and TP from simulated biogas slurry in the system could be realized as high as 93.22% and 82.38%, respectively. After stable operation of the system for 37 days, the microbial richness of the algal-bacterial symbiotic biofilm increased, and the nitrogen removal efficiency was higher than 90%. In biofilm, Thauera, with an relative abundance of 56.42%, played a dominant role in denitrification, while the relative abundance of AOB was reduced to 5.65%, and the abundance of NOB was very low. The high efficiency of nitrogen removal was realized through the shortcut nitrification-denitrification process (about 88.46%) and biological assimilation (about 6.79%) in shortcut nitrogen removal system for algal-bacterial symbiosis. About 60.5% of the carbon source for denitrification was saved, compared with traditional biological denitrification technology.
  • [1]
    LI S N, CHU Y H, XIE P, et al. Insights into the microalgae-bacteria consortia treating swine wastewater: symbiotic mechanism and resistance genes analysis[J]. Bioresource Technology,2022,349:126892-126892.
    [2]
    汪晓军,陈永兴,陈振国.厌氧氨氧化及其处理低碳氮比氨氮废水的研究进展[J].工业水处理,2022,42(11):25-31.
    [3]
    袁强军,张宏星,陈芳媛.不同低碳氮比废水中好氧颗粒污泥的长期运行稳定性[J].环境科学,2020,41(10):4661-4668.
    [4]
    WANG C, QIAO S, ZHOU J T. Strategy of nitrate removal in anaerobic ammonia oxidation-dependent processes[J]. Chemosphere,2023,313.
    [5]
    LIU X G, KIM M, NAKHLA G, et al. Partial nitrification-reactor configurations, and operational conditions: performance analysis[J]. Journal of Environmental Chemical Engineering, 2020,8(4).
    [6]
    SEUNTJENS D, HAN M, KERCKHOF F M, et al. Pinpointing wastewater and process parameters controlling the AOB to NOB activity ratio in sewage treatment plants[J]. Water Research, 2018, 138: 37-46.
    [7]
    祝贵兵,彭永臻,郭建华.短程硝化反硝化生物脱氮技术[J].哈尔滨工业大学学报,2008(10):1552-1557.
    [8]
    REN S, WANG Z, JIANG H, et al. Stable nitritation of mature landfill leachate via in-situ selective inhibition by free nitrous acid[J]. Bioresource Technology,2021,340:125647-125647.
    [9]
    LI Q, XU Y F, LIANG C Z, Peng L, et al. Nitrogen removal by algal-bacterial consortium during mainstream wastewater treatment: transformation mechanisms and potential N2O mitigation[J]. Water Research,2023,235:119890-119890.
    [10]
    WANG J L, DENG Y F, CHEN W, et al. Revealing the role of algae in algae enhanced bacteria consortia for municipal wastewater treatment: performance, characteristics, and microbial pathways[J]. Journal of Water Process Engineering,2023,53.
    [11]
    刘雨雪.藻菌生物膜处理水产养殖废水及其饲料化潜力研究[D]. 武汉:华中科技大学,2021.
    [12]
    陈文婷.固定化藻菌强化榨菜废水尾水的处理[D]. 重庆:重庆大学,2020.
    [13]
    WEI D, XUE X, YAN L, et al. Effect of influent ammonium concentration on the shift of full nitritation to partial nitrification in a sequencing batch reactor at ambient temperature[J]. Chemical Engineering Journal,2014,235:19-26.
    [14]
    CHEN C, SONG Y H, YUAN Y C. The Operating Characteristics of Partial Nitrification by Controlling pH and Alkalinity[J]. Water,2021,13(3):286-286.
    [15]
    VILLAVERDE S, GARCÍA E P A, FDZ P F. Influence of pH over nitrifying biofilm activity in submerged biofilters[J]. Water Research, 1997,31(5):1180-1186.
    [16]
    VADIVELU V M, YUAN Z G, FUX C, et al The inhibitory effects of free nitrous acid on the energy generation and growth processes of an enriched Nitrobacter culture[J]. Environmental Science & Technology,2006,40(14): 4442-4448.
    [17]
    LU S M, LIU X G, LIU C, et al. Influence of photoinhibition on nitrification by ammonia-oxidizing microorganisms in aquatic ecosystems[J]. Reviews in Environmental Science and Bio/Technology, 2020,19(3):531-542.
    [18]
    董昆,李奕燃,李晓强,等.游离氨对生物脱氮硝化过程细菌种群结构的影响[J].环境化学,2022,41(8):2742-2751.
    [19]
    SIRIPONG S, RITTMANN B E. Diversity study of nitrifying bacteria in full-scale municipal wastewater treatment plants[J].Water Research,2007,41(5):1110-1120.
    [20]
    CHEN H C, CHEN Z G, ZHOU S W, et al. Efficient partial nitritation performance of real printed circuit board tail wastewater by a zeolite biological fixed bed reactor[J]. Journal of Water Process Engineering,2023,53.
    [21]
    OLIVER T I, MOHAMED S Z, RANIA A H, et al Long-term aerobic granular sludge stability through anaerobic slow feeding, fixed feast-famine period ratio, and fixed SRT[J]. Journal of Environmental Chemical Engineering,2020,8(2).
    [22]
    BENNETT K, SADLER N C, WRIGHT A T, et al. Activity-Based Protein Profiling of Ammonia Monooxygenase in Nitrosomonas europaea[J]. Applied and Environmental Microbiology,2016,82(8):2270-2279.
    [23]
    WANG X X, FANG F, CHEN Y P, et al. N2O micro-profiles in biofilm from a one-stage autotrophic nitrogen removal system by microelectrode[J]. Chemosphere,2017,175:482-489.
    [24]
    ABHISHEK D, PRADYUT K, SUNITA A. Two stage treatability and biokinetic study of dairy wastewater using bacterial consortium and microalgae[J]. Biocatalysis and Agricultural Biotechnology,2022,43.
    [25]
    ALCÁNTARA C, MUÑOZ R, NORVILL Z, et al. Nitrous oxide emissions from high rate algal ponds treating domestic wastewater[J]. Bioresource Technology,2015,177:110-117.
    [26]
    SHI F, LIU Z L, LI J L, et al. Alterations in microbial community during the remediation of a black-odorous stream by acclimated composite microorganisms[J].Journal of Environmental Sciences, 2022,118(8):181-193.
    [27]
    ZENG D W, MIAO J, WU G X, et al. Nitrogen removal, microbial community and electron transport in an integrated nitrification and denitrification system for ammonium-rich wastewater treatment[J].International Biodeterioration & Biodegradation,2018,133:202-209.
    [28]
    包美玲,胡智泉,吴滔,等. 藻菌共生光序批式生物膜反应器短程脱氮效能研究[J]. 水处理技术, 2021, 47 (6): 58-63

    ,69.
  • Relative Articles

    [1]CHEN Yi, LI Longguo, BAI Ting, CHEN Meng, HUANG Yanchun, FU Bin, LI Naiwen. EVALUATION AND CORRELATION ANALYSIS OF WATER/SEDIMENT POLLUTION STATUS IN CHENGDU SECTION OF THE TUOJIANG RIVER BASIN[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(7): 144-152. doi: 10.13205/j.hjgc.202407016
    [2]HUANG Junlin, LIU Huangrui, YAN Haihu, XI Yunhao, LI Xiang, ZHU Nengwu. POLLUTION CHARACTERISTICS AND RISK ASSESSMENT OF HEAVY METALS AND CHLORIDES IN SOLID WASTE INCINERATION RESIDUES[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(8): 134-141. doi: 10.13205/j.hjgc.202408016
    [3]LIU Wei, YI Yuanrong, LI Chunhui, LI Jie, DINA Jaabay. MECHANISM OF SOLIDIFICATION OF HEAVY METALS (Zn, Cd) BY LADLE FURNACE SLAG-FLY ASH BASED GEOPOLYMERS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(6): 127-135. doi: 10.13205/j.hjgc.202406015
    [4]XU Yang, YAN Yulong, DUAN Xiaolin, WU Jing, PENG Lin, ZHANG Xiangyu, NIU Yueyuan, LIU Zhuocheng, ZHANG Dayu. CHARACTERISTICS AND SOURCE ANALYSIS OF HALOCARBONS IN SUMMER AT HIGH ALTITUDE BACKGROUND SITE OF NAMCO, TIBETAN PLATEAU[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(4): 55-62. doi: 10.13205/j.hjgc.202304008
    [5]ZHANG Yibing, LIANG Yiqun, ZHANG Yuan, FANG Yinxiang, NIU Hongya, FAN Jingsen. SOURCE APPORTIONMENT AND ECOLOGICAL RISK ASSESSMENT OF HEAVY METALS IN PM2.5 IN THE FENGFENG MINING AREA IN 2017—2019[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 242-250. doi: 10.13205/j.hjgc.202308031
    [6]ZHANG Yaning, ZHU Weihuang, DONG Ying, WU Xijun, LIU Jing. EFFECT OF REDOX CONDITION AND MICROBIAL ACTION ON HEAVY METALS TRANSFORMATION IN RESERVOIR SEDIMENTS[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(6): 101-108. doi: 10.13205/j.hjgc.202306014
    [7]CHEN Xuejuan, GAO Fang, WANG Qing, PANG Bo, XIE Yiliang, CUI Baoshan, YUE Xiupeng, SONG Jianbin. DISTRIBUTION CHARACTERISTICS AND POTENTIAL RISK OF HEAVY METALS IN WETLAND FRESHWATER RESTORATION AREA OF THE YELLOW RIVER DELTA[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(1): 232-239. doi: 10.13205/j.hjgc.202301028
    [8]LI Dou, WANG Yan, LIU Ruhai, SUN Haolin, YIN Pingping, ZHOU Xuyuan, MO Bing, LI Dongting. TEMPORAL VARIATION, SOURCE ANALYSIS AND ENVIRONMENTAL EFFECTS OF WATER-SOLUBLE IONS IN TSP IN QINGDAO[J]. ENVIRONMENTAL ENGINEERING , 2023, 41(8): 109-116,126. doi: 10.13205/j.hjgc.202308014
    [9]SHEN Song, LIU Lei, WEN Wei, XING Yi, SU Wei, SUN Jiaqi. POLLUTION CHARACTERIZATION AND SOURCE ANALYSIS OF CARBON COMPONENTS OF PM2.5 IN BEIJING AND SURROUNDING AREAS IN SUMMER[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 71-80. doi: 10.13205/j.hjgc.202202012
    [10]LU Yifan, LU Yin, CAI Hui, SUN Shoujun, SHI Weilin. POLLUTION ANALYSIS AND HEALTH RISK ASSESSMENT OF HEAVY METALS IN FIELD LEFT BY A LEAD-ACID BATTERY FACTORY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(1): 135-140,189. doi: 10.13205/j.hjgc.202201020
    [11]NI Haifeng, DAN Zeng, ZHOU Wenwu, ZHOU Peng, XU Fei, YANG Tao, MENG Dean, CHEN Guanyi. CHARACTERISTICS ANALYSIS AND RISK ASSESSMENT OF HEAVY METALS OF WASTE INCINERATION FLY ASH IN LHASA[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(3): 89-93,131. doi: 10.13205/j.hjgc.202203014
    [12]CUI Feijian, QIN Guangxiong, ZENG Hailong, HUANG Zhiwei, LI Wenjing, YANG Hanjie, HU Yanfang, FANG Huaiyang, ZENG Fantang, DU Hongwei. SPATIAL DISTRIBUTION CHARACTERISTICS AND POLLUTION ASSESSMENT OF NITROGEN, PHOSPHORUS AND HEAVY METAL IN SURFACE SEDIMENTS OF HEAVILY POLLUTED TRIBUTARIES OF SHAHE RIVER BASIN[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(1): 110-116. doi: 10.13205/j.hjgc.202201016
    [13]LI Yan-xue, ZHANG Meng-zhu, SHU Sha-sha, ZOU Jun-han, JIAO Wei, ZHOU Jun-yu. QUANTITATIVE IDENTIFICATION OF ANTHROPOGENIC HEAVY METAL SOURCES IN FARMLAND SOIL BASED ON ENRICHMENT FACTOR AND MLR-APCS MODEL[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(9): 173-177,232. doi: 10.13205/j.hjgc.202209023
    [14]MA Tao, SONG Jiang-min, LIU Qun-qun, SHENG Yan-qing. COMPARISON OF ECOLOGICAL RISK ASSESSMENT OF HEAVY METALS IN DREDGED SEDIMENT TREATED BY DIFFERENT METHODS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(2): 141-146,152. doi: 10.13205/j.hjgc.202102023
    [15]WAN Ling, XIANG Song, NIU Yong, HUANG Tian-yin, WANG Yong-tao, PANG Yan. DISTRIBUTION CHARACTERISTICS AND POLLUTION ASSESSMENT OF NITROGEN, PHOSPHORUS AND ORGANIC MATTERS IN SURFACE SEDIMENTS OF THE NIUGU RIVER[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 174-180. doi: 10.13205/j.hjgc.202101027
    [16]WANG Yu, ZHUANG Xu-ning, MAO Shao-hua, GU Wei-hua, BAI Jian-feng. ANALYSIS OF TOXIC AND HARMFUL METALS CONTENT AND ECOLOGICAL RISK IN WASTE LCD PANELS[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(1): 117-121. doi: 10.13205/j.hjgc.202001018
    [17]WU Qi-hao, JIANG Xin-quan, MA Xiao-li, CHEN Ping, CHEN Jing-run. CORRELATION ANALYSIS OF HEAVY METAL POLLUTION AND MAGNETIC SUSCEPTIBILITY IN SOIL OF A SEWAGE IRRIGATION AREA[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(9): 231-235,174. doi: 10.13205/j.hjgc.202009037
    [18]LIU Chun-yue, WANG Hui, BAI Ming-yue, ZHAO Yue-ming, WU Hao, WANG Xiao-xu, ZHAO Si-cong. RISK ASSESSMENT AND CHARACTERISTICS OF HEAVY METALS IN SURFACE SOIL OF OLD TOWN OF SHENYANG[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(1): 167-171. doi: 10.13205/j.hjgc.202001027
    [19]CHENG Shu-zhen, SUN Chang-shun, WANG Li-xiang, GUO Xin-chao, LI Yuan-han. ANALYSIS ON CONTENT CHARACTERISTICS OF NUTRIENTS AND HEAVY METALS IN URBAN SLUDGE OF SHAANXI PROVINCE[J]. ENVIRONMENTAL ENGINEERING , 2020, 38(5): 65-69. doi: 10.13205/j.hjgc.202005012
  • Cited by

    Periodical cited type(0)

    Other cited types(1)

  • Created with Highcharts 5.0.7Amount of accessChart context menuAbstract Views, HTML Views, PDF Downloads StatisticsAbstract ViewsHTML ViewsPDF Downloads2024-052024-062024-072024-082024-092024-102024-112024-122025-012025-022025-032025-04051015202530
    Created with Highcharts 5.0.7Chart context menuAccess Class DistributionFULLTEXT: 23.0 %FULLTEXT: 23.0 %META: 75.5 %META: 75.5 %PDF: 1.6 %PDF: 1.6 %FULLTEXTMETAPDF
    Created with Highcharts 5.0.7Chart context menuAccess Area Distribution其他: 6.8 %其他: 6.8 %China: 2.3 %China: 2.3 %[]: 0.2 %[]: 0.2 %上海: 0.3 %上海: 0.3 %东莞: 0.3 %东莞: 0.3 %临汾: 0.2 %临汾: 0.2 %北京: 1.0 %北京: 1.0 %南京: 0.5 %南京: 0.5 %南宁: 0.9 %南宁: 0.9 %南里奥格兰德州: 1.0 %南里奥格兰德州: 1.0 %台州: 0.2 %台州: 0.2 %嘉兴: 1.0 %嘉兴: 1.0 %天津: 0.5 %天津: 0.5 %宝鸡: 0.7 %宝鸡: 0.7 %宿迁: 0.2 %宿迁: 0.2 %巴中: 0.5 %巴中: 0.5 %常德: 0.2 %常德: 0.2 %广州: 0.7 %广州: 0.7 %廊坊: 0.2 %廊坊: 0.2 %张家口: 0.3 %张家口: 0.3 %成都: 0.2 %成都: 0.2 %昆明: 0.2 %昆明: 0.2 %晋城: 0.3 %晋城: 0.3 %朝阳: 0.2 %朝阳: 0.2 %柳州: 0.2 %柳州: 0.2 %桂林: 1.4 %桂林: 1.4 %武汉: 0.2 %武汉: 0.2 %洛阳: 0.2 %洛阳: 0.2 %济源: 0.2 %济源: 0.2 %滁州: 0.2 %滁州: 0.2 %漯河: 0.5 %漯河: 0.5 %潍坊: 0.2 %潍坊: 0.2 %甘南: 0.3 %甘南: 0.3 %石家庄: 0.2 %石家庄: 0.2 %聊城: 0.3 %聊城: 0.3 %芒廷维尤: 49.7 %芒廷维尤: 49.7 %芝加哥: 0.5 %芝加哥: 0.5 %苏州: 0.2 %苏州: 0.2 %西宁: 22.3 %西宁: 22.3 %西安: 0.2 %西安: 0.2 %贵阳: 0.2 %贵阳: 0.2 %运城: 1.4 %运城: 1.4 %遵义: 0.2 %遵义: 0.2 %邯郸: 0.3 %邯郸: 0.3 %郑州: 0.5 %郑州: 0.5 %重庆: 0.2 %重庆: 0.2 %钦州: 0.3 %钦州: 0.3 %长春: 0.2 %长春: 0.2 %长治: 0.2 %长治: 0.2 %阳泉: 0.3 %阳泉: 0.3 %青岛: 0.5 %青岛: 0.5 %其他China[]上海东莞临汾北京南京南宁南里奥格兰德州台州嘉兴天津宝鸡宿迁巴中常德广州廊坊张家口成都昆明晋城朝阳柳州桂林武汉洛阳济源滁州漯河潍坊甘南石家庄聊城芒廷维尤芝加哥苏州西宁西安贵阳运城遵义邯郸郑州重庆钦州长春长治阳泉青岛

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (115) PDF downloads(1) Cited by(1)
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return