MODEL CONSTRUCTION AND EFFECT INVESTIGATION OF GARDEN WASTE DEGRADING BACTERIAL AGENT

CHEN Xin; GAO Yu; LI Yihan; CHEN Hongwei; HU Cuiying; QIAN Wei

doi:10.13205/j.hjgc.202408021

Volume 42 Issue 8

Aug. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(8): 173-183

CHEN Xin, GAO Yu, LI Yihan, CHEN Hongwei, HU Cuiying, QIAN Wei. MODEL CONSTRUCTION AND EFFECT INVESTIGATION OF GARDEN WASTE DEGRADING BACTERIAL AGENT[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(8): 173-183. doi: 10.13205/j.hjgc.202408021

Citation:

CHEN Xin, GAO Yu, LI Yihan, CHEN Hongwei, HU Cuiying, QIAN Wei. MODEL CONSTRUCTION AND EFFECT INVESTIGATION OF GARDEN WASTE DEGRADING BACTERIAL AGENT[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(8): 173-183. doi: 10.13205/j.hjgc.202408021

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MODEL CONSTRUCTION AND EFFECT INVESTIGATION OF GARDEN WASTE DEGRADING BACTERIAL AGENT

doi: 10.13205/j.hjgc.202408021

1. Suzhou University of Science and Technology, Suzhou 215000, China;
2. Zhangjiagang City Garden Nursery Landscape Technology Research Institute, Zhangjiagang 215600, China

Received Date: 2023-09-22
Available Online: 2024-12-02

Abstract

Abstract

To investigate the feasibility of the mixture design methodology applied to the construction of composite inoculum, and the optimization of components to address the current problems of long composting cycles and low wood fiber conversion efficiency of garden waste, the degrading strains from the GW compost samples and their interrelationships were identified, using isolation culture and antagonism experiments. The non-antagonistic strains were mixed in different proportions using the mixture design method and their enzyme activities were measured, after which the mathematical models between the proportions of different strains and the enzyme activities were established. The results showed the composite consisted of Bacillus tequilensis, Bacillus velezensis, and Bacillus oryzaecorticis, and their optimum ratio was 28.95%: 39.63%: 31.42%. Meanwhile, in the degradation experiment using GW as the carbon source, the maximum enzyme activity of laccase was 47.25 U/L, lignin peroxidase was 66.67 U/L, manganese peroxidase was 99.65 U/L, carboxymethyl cellulase was 90.66 U/mL, and filter paper activity was 48.44 U/mL. The degradation rates of cellulose and lignin on the 18th day could reach 13.48% and 9.73%, respectively. The composite inoculum constructed based on the mixture design showed superior short-term enzyme activity production and biodegradation capacity, which is conducive to the application of GW composting.
- garden waste,
- lignocellulose-degrading bacteria,
- biodegradation,
- composite inoculum,
- mixture design

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References

[1]	HUANG Y H, CHEN X H, LI Q F, et al. Fungal community enhanced humification and influenced by heavy metals in industrial-scale hyperthermophilic composting of municipal sludge[J]. Bioresource Technology, 2022, 360: 127523.
[2]	REYES-TORRES M, OVIEDO-OCAñA E R, DOMINGUEZ I, et al. A systematic review on the composting of green waste: feedstock quality and optimization strategies[J]. Waste Management, 2018, 77: 486-499.
[3]	MORI T, TSUBOI Y, ISHIDA N, et al. Multidimensional high-resolution magic angle spinning and solution-state NMR characterization of 13C-labeled plant metabolites and lignocellulose[J]. Scientific Reports, 2015, 5: 11848.
[4]	DESSIE W, XIN F X, ZHANG W M, et al. Inhibitory effects of lignocellulose pretreatment degradation products (hydroxymethylfurfural and furfural) on succinic acid producing Actinobacillus succinogenes[J]. Biochemical Engineering Journal, 2019, 150: 107263.
[5]	FILIPOWICZ N, CIES＇LIN＇SKI H. A rapid and simple method for screening microorganisms with a potential for catechol biodegradation[J]. International Journal of Environmental Research, 2020, 14: 87-92.
[6]	ZHANG Y, ZHAO Y, CHEN Y N, et al. A regulating method for reducing nitrogen loss based on enriched ammonia-oxidizing bacteria during composting[J]. Bioresource Technology, 2016, 221: 276-283.
[7]	ALKOAIK F, GHALY A E. Influence of dairy manure addition on the biological and thermal kinetics of composting of greenhouse tomato plant residues[J]. Waste Management, 2006, 26: 902-913.
[8]	胡亚冬, 范德朋, 孔维杰, 等. 复合菌剂强化餐厨垃圾好氧生物处理性能研究[J]. 环境工程, 2022, 40(4): 97-105.
[9]	LI M, PENG X Y, ZHAO Y C, et al. Microbial inoculum with leachate recirculated cultivation for the enhancement of OFMSW composting[J]. Journal of Hazardous Materials, 2008, 153: 885-891.
[10]	NIKZADE V, TEHRANI M M, SAADATMAND-Tarzjan M. Optimization of low-cholesterol-low-fat mayonnaise formulation: effect of using soy milk and some stabilizer by a mixture design approach[J]. Food Hydrocolloids, 2012, 28: 344-352.
[11]	PAULI E D, MALTA G B, SANCHEZ P M, et al. Mixture design analysis of solvent extractor effects on epicatechin, epigallocatechin gallate, epigallocatechin and antioxidant activities of the Camellia sinensis L. leaves[J]. Analytical Chemistry Research, 2014, 2: 23-29.
[12]	AOUAN B, ALEHYEN S, FADIL M, et al. Characteristics, microstructures, and optimization of the geopolymer paste based on three aluminosilicate materials using a mixture design methodology[J]. Construction and Building Materials, 2023, 384: 131475.
[13]	M'HIR S, ZIADI M, MEJRI A, et al. Mixture of whey-milk and palm sap for novel kefir beverage using simplex-centroid mixture design[J]. Kuwait Journal of Science, 2023.
[14]	SI J Y, YANG C R, MA W J, et al. Screen of high-efficiency cellulose degrading strains and effects on tea residues dietary fiber modification: structural properties and adsorption capacities[J]. International Journal of Biological Macromolecules, 2022, 220: 337-347.
[15]	YU K F, SUN X Y, LI S Y, et al. Application of quadratic regression orthogonal design to develop a composite inoculum for promoting lignocellulose degradation during green waste composting[J]. Waste Management, 2018, 79: 443-453.
[16]	XU X Q, WU P, WANG T Z, et al. Synergistic effects of surfactant-assisted biodegradation of wheat straw and production of polysaccharides by Inonotus obliquus under submerged fermentation[J]. Bioresource Technology, 2019, 278: 43-50.
[17]	PINTO P A, DIAS A A, FRAGA I, et al. Influence of ligninolytic enzymes on straw saccharification during fungal pretreatment[J]. Bioresource Technology, 2012, 111: 261-267.
[18]	JING R X, LI N, WANG W P, et al. An endophytic strain JK of genus bacillus isolated from the seeds of super hybrid rice (Oryza sativa L., Shenliangyou 5814) has antagonistic activity against rice blast pathogen[J]. Microbial Pathogenesis, 2020, 147: 104422.
[19]	MOLINA-SANTIAGO C, PEARSON J R, NAVARRO Y, et al. The extracellular matrix protects Bacillus subtilis colonies from Pseudomonas invasion and modulates plant co-colonization[J]. Nature Communications, 2019, 10: 1919.
[20]	GONG X Q, LI S Y, SUN X Y, et al. Maturation of green waste compost as affected by inoculation with the white-rot fungi Trametes versicolor and Phanerochaete chrysosporium[J]. Environmental Technology, 2017, 38: 872-879.
[21]	ZHU P C, LI Y C, GAO Y F, et al. Insight into the effect of nitrogen-rich substrates on the community structure and the co-occurrence network of thermophiles during lignocellulose-based composting[J]. Bioresource Technology, 2021, 319: 124111.
[22]	NAWAZ M Z, SHANG H R, SUN J Z, et al. Genomic insights into the metabolic potential of a novel lignin-degrading and polyhydroxyalkanoates producing bacterium Pseudomonas sp. Hu109A[J]. Chemosphere, 2023, 310: 136754.
[23]	SOOBHANY N, GUNASEE S, RAGO Y P, et al. Spectroscopic, thermogravimetric and structural characterization analyses for comparing municipal solid waste composts and vermicomposts stability and maturity[J]. Bioresource Technology, 2017, 236: 11-19.
[24]	AIT BADDI G, HAFIDI M, CEGARRA J, et al. Characterization of fulvic acids by elemental and spectroscopic (FTIR and 13C-NMR) analyses during composting of olive mill wastes plus straw[J]. Bioresource Technology, 2004, 93: 285-290.
[25]	EL FELS L, ZAMAMA M, EL ASLI A, et al. Assessment of biotransformation of organic matter during co-composting of sewage sludge-lignocelullosic waste by chemical, FTIR analyses, and phytotoxicity tests[J]. International Biodeterioration & Biodegradation, 2014, 87: 128-137.
[26]	ABIDI N, CABRALES L, HAIGLER C H. Changes in the cell wall and cellulose content of developing cotton fibers investigated by FTIR spectroscopy[J]. Carbohydrate Polymers, 2014, 100: 9-16.
[27]	EBISSA D T, TESFAYE T, WORKU D, et al. Characterization and optimization of the properties of untreated high land bamboo fibres[J]. Heliyon, 2022, 8: e09856.
[28]	VIERA R G P, FILHO G R, DE ASSUNÇÃO R M N, et al. Synthesis and characterization of methylcellulose from sugar cane bagasse cellulose[J]. Carbohydrate Polymers, 2007, 67: 182-189.
[29]	XU G Q, WANG L H, LIU J L, et al. FTIR and XPS analysis of the changes in bamboo chemical structure decayed by white-rot and brown-rot fungi[J]. Applied Surface Science, 2013, 280: 799-805.
[30]	MU D C, QU F T, ZHU Z C, et al. Effect of Maillard reaction on the formation of humic acid during thermophilic phase of aerobic fermentation[J]. Bioresource Technology, 2022, 357: 127362.
[31]	LI K M, SHAHAB A, LI J Y, et al. Compost-derived humic and fulvic acid coupling with Shewanella oneidensis MR-1 for the bioreduction of Cr(Ⅵ)[J]. Journal of Environmental Management, 2023, 345: 118596.
[32]	MACHADO W, FRANCHINI J C, DE FÁTIMA Guimarães M, et al. Spectroscopic characterization of humic and fulvic acids in soil aggregates, Brazil[J]. Heliyon, 2020, 6: e04078.
[33]	CUI D Y, TAN W B, YUE D B, et al. Reduction capacity of humic acid and its association with the evolution of redox structures during composting[J]. Waste Management, 2022, 153: 188-196.
[34]	ZHOU H, ZHU H, REN Z, et al. Efficacy of Bacillus tequilensis strain JN-369 to biocontrol of rice blast and enhance rice growth[J]. Biological Control, 2021, 160: 104652.
[35]	SAWANT S S, SONG J, SEO H J. Study of new biocontrol bacterial agent Bacillus velezensis S41L against Rosellinia necatrix[J]. Biological Control, 2023, 185: 105305.
[36]	SUN T, YANG Y, DUAN K, et al. Biodiversity of endophytic microbes in diverse tea Chrysanthemum cultivars and their potential promoting effects on plant growth and quality[J]. Biology, 2023, 12: 986.

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