SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA

HUANG Jialiang; MU Lan; WU Wanting; PENG Hao; TAO Junyu; SONG Yingjin; SHI Yan; CHEN Guanyi

doi:10.13205/j.hjgc.202409030

Volume 42 Issue 9

Sep. 2024

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HUANG Jialiang, MU Lan, WU Wanting, PENG Hao, TAO Junyu, SONG Yingjin, SHI Yan, CHEN Guanyi. SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(9): 301-310. doi: 10.13205/j.hjgc.202409030

Citation:

HUANG Jialiang, MU Lan, WU Wanting, PENG Hao, TAO Junyu, SONG Yingjin, SHI Yan, CHEN Guanyi. SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(9): 301-310. doi: 10.13205/j.hjgc.202409030

Citation:

HUANG Jialiang, MU Lan, WU Wanting, PENG Hao, TAO Junyu, SONG Yingjin, SHI Yan, CHEN Guanyi. SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(9): 301-310. doi: 10.13205/j.hjgc.202409030

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SCREENING AND HYDROGEN PRODUCTION PERFORMANCE OF FOUR MIXED PHOTOSYNTHETIC ANAEROBIC HYDROGEN-PRODUCING BACTERIAL FLORA

doi: 10.13205/j.hjgc.202409030

HUANG Jialiang^1,3,
MU Lan^{2,3
,
,},
WU Wanting^1,3,
PENG Hao⁴,
TAO Junyu^2,3,
SONG Yingjin⁴,
SHI Yan²,
CHEN Guanyi^1,2

1. The College of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China;
2. The College of Mechanical Engineering, Tianjin University of Commerce, Tianjin 300134, China;
3. Interdisciplinary Innovation Lab for Environment and Energy, Tianjin University of Commerce, Tianjin 300134, China;
4. The College of Environmental Science and Engineering, Tianjin University, Tianjin 300350, China

Received Date: 2024-02-25
Available Online: 2024-12-02

Abstract

Abstract

The efficient utilization of biomass energy from a wide range of sources is beneficial to achieving the goal of sustainable green energy. In this study, the dark light mixed fermentation technology was used to enrich four kinds of dark light mixed bacterial flora from aerobic sludge, anaerobic sludge, lake mud and cow dung, and the anaerobic fermentation of glucose was used to produce hydrogen. In this study, microorganisms, hydrogen production, growth, and material change characteristics during fermentation were investigated. The 16S rRNA high-throughput results showed that all four flora were dark-light mixed colonies dominated by the genus Rhodopseudomonas sp., with a diversity of flora structures, and obvious differences among the four flora. Among the four dark-light hybrid flora, the hydrogen production performance of the cow dung hybrid flora was the best, with a cumulative hydrogen yield of (633.93±4.87) mL/L, a maximum hydrogen production rate of (8.55±0.31) mL/(L·h), and an energy conversion rate of 9.22%, with 7.32% of the electrons in the substrate flowed to the hydrogen, under the condition of 30 mmol/L glucose, 35 ℃, and 5000 lux. The results showed that the dark-light hybrid flora could efficiently utilize the substrate for hydrogen production by anaerobic fermentation.
- biological hydrogen production,
- dark-light coupled fermentation,
- dark-light mixed flora,
- photosynthetic bacterium,
- energy transferring efficiency,
- electron transfer

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References(40)

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