EFFECT OF COMBINATION OF RICE STRAW BIOCHAR AND <i>BACILLUS CEREUS</i> ON TRANSFORMATION OF SOIL HEAVY METAL SPECIATIONS AND MICROBIAL COMMUNITY

LI Zishan; HU Zhiwen; MEI Chuang; BAI Jinjing; ZENG Yan; XIAO Rongbo; WANG Peng; HUANG Fei

doi:10.13205/j.hjgc.202410020

Volume 42 Issue 10

Oct. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(10): 165-176

LI Zishan, HU Zhiwen, MEI Chuang, BAI Jinjing, ZENG Yan, XIAO Rongbo, WANG Peng, HUANG Fei. EFFECT OF COMBINATION OF RICE STRAW BIOCHAR AND BACILLUS CEREUS ON TRANSFORMATION OF SOIL HEAVY METAL SPECIATIONS AND MICROBIAL COMMUNITY[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(10): 165-176. doi: 10.13205/j.hjgc.202410020

Citation:

LI Zishan, HU Zhiwen, MEI Chuang, BAI Jinjing, ZENG Yan, XIAO Rongbo, WANG Peng, HUANG Fei. EFFECT OF COMBINATION OF RICE STRAW BIOCHAR AND BACILLUS CEREUS ON TRANSFORMATION OF SOIL HEAVY METAL SPECIATIONS AND MICROBIAL COMMUNITY[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(10): 165-176. doi: 10.13205/j.hjgc.202410020

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EFFECT OF COMBINATION OF RICE STRAW BIOCHAR AND BACILLUS CEREUS ON TRANSFORMATION OF SOIL HEAVY METAL SPECIATIONS AND MICROBIAL COMMUNITY

doi: 10.13205/j.hjgc.202410020

1. School of Environmental Science and Engineering, Guangdong University of Technology, Guangzhou 510006, China;
2. College of Environment and Ecology, Chongqing University, Chongqing 400044, China

Received Date: 2023-12-20
Available Online: 2024-11-30

Abstract

Abstract

To determine the combined effect of rice straw biochar and Bacillus on the transformation of heavy metal speciation and the mechanisms affecting microbial communities in Cu-Cd contaminated soil, the contents of different speciation of Cu and Cd in soil, soil chemical properties and enzyme activities were compared and analyzed through soil culture experiments. The structure composition and diversity response patterns of soil microbial communities were investigated by high-throughput sequencing technology. The results indicated that after biochar and microbial (BC+M) treatment, the acid-extractable Cu and Cd contents decreased significantly, and the contents decreased by 27.35% and 27.48%, respectively. BC+M significantly enhanced soil pH value, and enhanced soil available phosphorus, available potassium and catalase activity by 114.16%, 462.76% and 113.79%, respectively. Acid-extracted Cu and Cd were negatively correlated with pH, alkali-hydrolyzed nitrogen (AN), available phosphorus (AP), available potassium (AK), cation exchange capacity (CEC) and organic matter (SOM), but acid-extracted Cu was significantly negatively correlated with AK and AP (P<0.05), and acid-extracted Cd was significantly negatively correlated with AK and pH (P<0.05). The BC+M treatment significantly diversified the soil microbial communities, mainly increasing the abundance of dominant bacterial groups such as Chloroflexi, Actinobacteriota, Firmicutes, Acidobacteriota and Bacteroidota, which are known for strong resistance to heavy metals. Compared to fungal communities, bacterial communities respond more intensely to changes in soil environmental factors, especially Proteobacteria, Chloroflexi, Firmicutes, Acidobacteriota and Bacteroidota. Rice straw biochar and Bacillus primarily affect soil pH, AP, AK and the structure of the bacterial community, thereby facilitating the transformation of acid-extractable forms of Cu and Cd into other potentially less harmful states. This provides a theoretical reference for the microbial processes in soil heavy metal pollution remediation.
- biochar,
- Bacillus cereus,
- heavy metal,
- speciation,
- microbial community,
- soil

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References

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