IMPACT OF HIGH SALT ON HETEROTROPHIC COUPLED SULFUR AUTOTROPHIC (HSAD) PROCESS AND ECTOINE MITIGATION STRATEGY

LI Ran; HOU Yanan; LI Haibo; LIU Zhihua; HAN Yi; ZHANG Daohong; SONG Yuanyuan; GUO Jianbo; HUANG Cong

doi:10.13205/j.hjgc.202412011

Volume 42 Issue 12

Dec. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(12): 79-88

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LI Ran, HOU Yanan, LI Haibo, LIU Zhihua, HAN Yi, ZHANG Daohong, SONG Yuanyuan, GUO Jianbo, HUANG Cong. IMPACT OF HIGH SALT ON HETEROTROPHIC COUPLED SULFUR AUTOTROPHIC (HSAD) PROCESS AND ECTOINE MITIGATION STRATEGY[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(12): 79-88. doi: 10.13205/j.hjgc.202412011

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IMPACT OF HIGH SALT ON HETEROTROPHIC COUPLED SULFUR AUTOTROPHIC (HSAD) PROCESS AND ECTOINE MITIGATION STRATEGY

doi: 10.13205/j.hjgc.202412011

1. Tianjin Key Laboratory of Aquatic Science and Technology, Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
3. Taizhou University, Taizhou 318000, China

Received Date: 2024-03-07
Available Online: 2025-01-18

Abstract

Abstract

Increasing saline wastewater discharge poses a challenge to denitrification by heterotrophic and sulfur autotrophic denitrification (HSAD). The response mechanisms and feasible mitigation strategies of heterotrophic denitrifying microorganisms to high salt stress need to be further explored. In this study, it was found that a salinity of 2% increased denitrification efficiency. However, the denitrification performance of HSAD significantly decreased when salinity increased to 6%, NO^-₃-N removal decreased from 95.77% to 38.01%, and the contribution of sulfur autotrophic denitrification (SAD) continued to be higher than that of heterotrophic denitrification (HD). High salinity stimulation resulted in nicotinamide adenine dinucleotide content and adenosine triphosphate levels decreasing by 10.74%, 46.6% and 56.28% respectively, at 6% salinity. In addition, the reduced activities of denitrifying enzymes (nitrate reductase and nitrite reductase) and the decrease in denitrifying functional bacteria were also important factors contributing to the inhibition of HSAD denitrification under high salt stress. Notably, adding 250 mg/L ectoine at 6% salinity alleviated the salinity stress, and enhanced the denitrification efficiency of HSAD by promoting the secretion of extracellular polymeric substances and increasing the metabolic activities of HSAD microorganisms. Microbial community analysis showed that the abundance of HD-functional bacteria increased by 3.99% compared to the case without ectoine, highlighting the key regulatory role of ectoine on community succession and stability. The results of this study deepened the understanding of the inhibition mechanism of HSAD by high-salt wastewater, and provided a feasible technical solution for sulfur-based mixotrophic denitrification technology to alleviate salt stress.
- high salinity wastewater,
- heterotrophic and sulfur autotrophic denitrification,
- nitrogen removal,
- microbial metabolism,
- ectoine,
- microbial community

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References

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