EFFECT OF INFLUENT C/N RATIO ON PHOSPHORUS AND NITROGEN REMOVAL BY PARTIAL NITRIFICATION-DENITRIFICATION IN A<sup>2</sup>/O PROCESS WITH LOW DISSOLVED OXYGEN CONCENTRATION

LU Baoguang; XUE Shiyu; WU Chuandong; SUN Xueying; CHEN Chenyong; WANG Zhongqian; WEN Huifang; YUN Yupan; MIAO Zhijia

doi:10.13205/j.hjgc.202412012

Volume 42 Issue 12

Dec. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(12): 89-96

LU Baoguang, XUE Shiyu, WU Chuandong, SUN Xueying, CHEN Chenyong, WANG Zhongqian, WEN Huifang, YUN Yupan, MIAO Zhijia. EFFECT OF INFLUENT C/N RATIO ON PHOSPHORUS AND NITROGEN REMOVAL BY PARTIAL NITRIFICATION-DENITRIFICATION IN A2/O PROCESS WITH LOW DISSOLVED OXYGEN CONCENTRATION[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(12): 89-96. doi: 10.13205/j.hjgc.202412012

Citation:

LU Baoguang, XUE Shiyu, WU Chuandong, SUN Xueying, CHEN Chenyong, WANG Zhongqian, WEN Huifang, YUN Yupan, MIAO Zhijia. EFFECT OF INFLUENT C/N RATIO ON PHOSPHORUS AND NITROGEN REMOVAL BY PARTIAL NITRIFICATION-DENITRIFICATION IN A²/O PROCESS WITH LOW DISSOLVED OXYGEN CONCENTRATION[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(12): 89-96. doi: 10.13205/j.hjgc.202412012

Citation:

LU Baoguang, XUE Shiyu, WU Chuandong, SUN Xueying, CHEN Chenyong, WANG Zhongqian, WEN Huifang, YUN Yupan, MIAO Zhijia. EFFECT OF INFLUENT C/N RATIO ON PHOSPHORUS AND NITROGEN REMOVAL BY PARTIAL NITRIFICATION-DENITRIFICATION IN A²/O PROCESS WITH LOW DISSOLVED OXYGEN CONCENTRATION[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(12): 89-96. doi: 10.13205/j.hjgc.202412012

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EFFECT OF INFLUENT C/N RATIO ON PHOSPHORUS AND NITROGEN REMOVAL BY PARTIAL NITRIFICATION-DENITRIFICATION IN A²/O PROCESS WITH LOW DISSOLVED OXYGEN CONCENTRATION

doi: 10.13205/j.hjgc.202412012

1. Hebei Center for Ecological and Environmental Geology Research, Hebei GEO University, Shijiazhuang 050031, China;
2. Guangdong Yuehai Water Group Investment Co., Ltd., Shenzhen 518020, China;
3. National Engineering Research Center for Resources of Harbin Institute of Technology Co., Ltd., Harbin 150096, China

Received Date: 2024-01-23
Available Online: 2025-01-18

Abstract

Abstract

The phosphorus and nitrogen removal by partial nitrification-denitrification of A²/O with different C/N ratios (2.5~7.0), were investigated under a low dissolved oxygen condition (0.5mg/L). Results showed that 80% of the influent COD was used for synthesizing PHA in the anaerobic zone. When the C/N ratio was below 4.5, the effluent COD fluctuated with the influent. TN was mainly removed by partial nitrification, endogenous denitrification, and denitrifying phosphorus removal process. When the C/N ratio was 2.5 and 3.5, the TN removal both remained at a level of 80%. The average accumulation rate of nitrite reached 24.4% and 31.9% respectively, under a C/N ratio of 4.5 and 5.0. The effluent TN stably reached 10 mg/L below with a maximum removal rate of 85.3%. Therefore, Increasing the influent C/N appropriately could effectively improve TN removal. Phosphorus was mainly removed through the denitrifying phosphorus removal process. When the C/N ratio was 2.5, the phosphorus removal rate was reduced to 65%. When the C/N ratio was 3.5, the effluent phosphorus concentration was 0.3 mg/L, achieving the best phosphorus removal rate of 92.1%. The anoxic phosphorus uptake took up 74.6% of the total removal. It was found that when the influent C/N of the system was within 5.0 to 7.0, the coupled operation of denitrifying phosphorus removal, endogenous denitrification, partial nitrification, and aerobic phosphorus absorption in the system effectively achieved synchronous and efficient removal of organic matter, phosphorus, and nitrogen, with a removal rate of 84%, 85%, and 90%, respectively. Through the 16S rDNA high-throughput sequencing analysis, the microorganisms that play a role in nitrogen and phosphorus removal in the A²/O system were mainly polysaccharide bacterium, Candidatus Competitor, denitrifying bacteria, Azospira, Nitrobacteria, Nitrospira, Nitrosomonas, and denitrifying phosphorus accumulating bacteria, Hypomicrobium and Candidatus Accumulibacter.
- C/N,
- denitrifying phosphorus removal,
- endogenous denitrification,
- partial nitrification,
- low dissolved oxygen condition

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References(30)

References

[1]	华云洁,胡金财,施昱,等.Orbal 氧化沟工艺改造对脱氮效果的影响分析[J].环境工程,2023,41(增刊2):71-78.
[2]	岳志芳,李正,王彦隽.内蒙古某城镇污水处理厂提标改造工程实例[J].水处理技术,2024,50(4):144-147.
[3]	李志平,郭玉梅,邢美容,等. 昆明某污水处理厂Carrousel氧化沟硝化作用分析及升级改造[J].水处理技术,2014,40(10):123-127.
[4]	贾莉.池州市城东污水厂A²/O氧化沟工艺的运行分析[J].中国给水排水,2014,30(9):121-124.
[5]	崔涛,李胜,田敏,等.氧化沟型A²O工艺脱氮除磷性能评价[J].中国给水排水,2023,39(22):75-79.
[6]	乔海兵,赵志太,李帅军,等.污水厂氧化沟改造为A²O+MBBR+O₃工艺提标设计[J].中国给水排水,2022,38(7):60-65.
[7]	MANSE R R,GUJER W, SIEG R H,et al.Consequences of mass transfer effects on the kinetics of nitrifiers[J].Water Research,2005,39 (19): 4633-4642.
[8]	张杰,杨杰,李冬. AOA后置短时低氧曝气实现短程硝化反硝化除磷[J]. 哈尔滨工业大学学报, 2023,56(6):1-7.
[9]	甄建园,于德爽,王晓霞,等. 低C/N(<3)条件下SNEDPR系统启动及其脱氮除磷特性研究[J]. 中国环境科学,2018, 38(8): 2960-2967.
[10]	BAI X Y, MICHELLE M, MCKNIGHT B, et al. Nitrogen removal pathways during simultaneous nitrification, denitrification, and phosphorus removal under low temperature and dissolved oxygen conditions[J]. Bioresource Technology, 2022,354: 127177.
[11]	李炳荣,曹特特,王林,等. 低氧条件下 A²/O 工艺对城市污水脱氮处理的中试研究[J].中国环境科学,2019,39(1):134-140.
[12]	任丽芳,李晓庆,孙洪伟. 不同温度 An/A/O-SBR反硝化除磷及N₂O释放特性[J].环境工程,2023,41(12):107-115.
[13]	吕利平,李航,庞飞,等. 交替好氧/缺氧短程硝化反硝化工艺处理低 C/N 城市污水[J].环境工程学报,2020,14(6):1529-1536.
[14]	国家环境保护局《水和废水监测分析方法》编委会. 水和废水监测分析方法[M]. 北京: 中国环境科学出版社,2002: 252-354.
[15]	张淼, 彭永臻, 张建华, 等.进水 C/N 对 A²/O-BCO 工艺反硝化除磷特性的影响[J]. 中国环境科学, 2016,36(5):1366-1375.
[16]	PENG Y, GE S. Enhanced nutrient removal in three types of step feeding process from municipal wastewater[J]. Bioresource Technology, 2011, 102(11):6405-6413.
[17]	ZHANG M, PENG Y Z, WANG C, et al. Optimization denitrifying phosphorus removal at different hydraulic retention times in a novel anaerobic anoxic oxic-biological contact oxidation process[J].Biochemical Engineering Journal, 2016,106:26-36.
[18]	KUBA T, SMOLDERS G, LOOSDRECHT M C M, et al. Biological phosphorus removal from wastewater by anaerobic-anoxic sequencing batch reactor[J]. Water Science and Technology, 1993, 27(5/6):241-252.
[19]	REN S Q, LIU Y R, HE Y Y, et al. Mathematical modeling of the dynamic effect of denitrifying glycogen-accumulating organisms on nitrous oxide production during denitrifying phosphorus removal[J] Chemical Engineering Journal, 2023,453:1-12.
[20]	CHUNG T S, ZHANG S, WANG K Y, et al. Forward osmosis processes: yesterday, today and tomorrow[J]. Desalination,2012,287(2):78-81.
[21]	HANAKI K, WANTAWIN C, OHGAKI S. Nitrification at low-levels of dissolved-oxygen with and without organic loading in a suspended-growth reactor[J]. Water Research, 1990, 24(3):297-302.
[22]	CAMEJO P Y, OWEN B R, Martirano J, et al. Candidatus Accumulibacter phosphatis clades enriched under cyclic anaerobic and microaerobic conditions simultaneously use different electron acceptors[J]. Water Research, 2016, 102:125-137.
[23]	SKENNERTON C T, BARR J J, SLATER F R, et al. Expanding our view of genomic diversity in Candidatus Accumulibacter clades: metabolic plasticity in Accumulibacter clades[J]. Environmental Microbiology, 2015, 17(5):1574-1585.
[24]	LEE D S, JEON C O, PARK J M. Biological nitrogen removal with enhanced phosphate uptake in a sequencing batch reactor using single sludge system[J]. Water Research, 2001, 35(16):3968-3976.
[25]	RIBERA-GUARDIA A, MARQUES R, ARANGIO C, et al. Distinctive denitrifying capabilities lead to differences in N₂O production by denitrifying polyphosphate accumulating organisms and denitrifying glycogen accumulating organisms[J]. Bioresource Technology, 2016, 219:106-113.
[26]	LI C, ZHANG J, LIANG S, et al. Nitrous oxide generation in denitrifying phosphorus removal process: main causes and control measures[J]. Environmental Science and Pollution Research, 2013, 20(8):5353-5360.
[27]	MIAO Z J, LI D, GUO S, et al. Effect of Free nitrous acid on nitrous oxide production and denitrifying phosphorus removal by polyphosphorus-accumulating organisms in wastewater treatment[J]. BioMed Research International, 2018, 26:9192607.
[28]	WINKLER M, BASSIN J P, KLEEREBEZEM R, et al. Selective sludge removal in a segregated aerobic granular biomass system as a strategy to control PAO-GAO competition at high temperatures[J]. Water Research, 2011, 45(11):3291-3299.
[29]	ZENG W, BAI X L, ZHANG L M, et al. Population dynamics of nitrifying bacteria for nitritation achieved in Johannesburg (JHB) process treating municipal wastewater[J]. Bioresource Technology, 2014, 162:30-37.
[30]	GU X, LENG J T, ZHU J T, et al. Influence mechanism of C/N ratio on heterotrophic nitrification-aerobic denitrification process[J]. Bioresource Technology, 2022, 343:126116.