Nitrogen removal performance and microbial biofilm on different plant stems in surface flow constructed wetlands

ZHAO Yiwei; YANG Zenghao; XU Hongbin; LI Guoqiang; FANG Yingke

doi:10.13205/j.hjgc.202502005

Volume 43 Issue 2

Feb. 2025

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ZHAO Yiwei, YANG Zenghao, XU Hongbin, LI Guoqiang, FANG Yingke. Nitrogen removal performance and microbial biofilm on different plant stems in surface flow constructed wetlands[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(2): 39-46. doi: 10.13205/j.hjgc.202502005

Citation:

ZHAO Yiwei, YANG Zenghao, XU Hongbin, LI Guoqiang, FANG Yingke. Nitrogen removal performance and microbial biofilm on different plant stems in surface flow constructed wetlands[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(2): 39-46. doi: 10.13205/j.hjgc.202502005

Citation:

ZHAO Yiwei, YANG Zenghao, XU Hongbin, LI Guoqiang, FANG Yingke. Nitrogen removal performance and microbial biofilm on different plant stems in surface flow constructed wetlands[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(2): 39-46. doi: 10.13205/j.hjgc.202502005

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Nitrogen removal performance and microbial biofilm on different plant stems in surface flow constructed wetlands

doi: 10.13205/j.hjgc.202502005

School of Ecology and Environment, Zhengzhou University, Zhengzhou 450001, China

Received Date: 2024-09-01
Accepted Date: 2024-11-11
Rev Recd Date: 2024-09-18

Abstract

Abstract

Microorganisms play a crucial role in the water purification process of constructed wetlands. In surface flow constructed wetlands, a large number of microorganisms are attached to the stems of emergent plants, which are closely related to wastewater treatment performance. However, research on the microorganisms on plant stems is still limited. This study established two surface flow constructed wetland systems composed of different plant mixtures (Iris pseudacorus + Canna indica, calamus + Cyperus alternifolius) and one surface flow constructed wetland composed of a single plant species(schoenoplectus), to investigate their nitrogen removal effects from wastewater. By examining the microbial diversity and community structure on the stems of four common wetland plants (Schoenoplectus, calamus, Canna and Cyperus alternifolius), the study analyzed the specificity of microbial communities on different wetland plants and clarified the microbial mechanisms of nitrogen removal in conjunction with the heterotrophic performance tests of plant stems. Results indicated that stem-associated microorganisms contributed approximately 0.25 to 0.6 mg/g fresh weight to the removal of nitrate nitrogen. Schoenoplectus exhibited the best removal efficiency for both ammonia and nitrate nitrogen, with its stem microorganisms contributing 0.58 mg/g, and its microbial community showing higher diversity and abundance than the other plants. From an overall pollutants removal perspective, the combination of Iris pseudacorus and Canna indica was identified as the optimal treatment combination, achieving an ammonia nitrogen removal rate of 83.8% and a total nitrogen removal rate of 51.9%. The predominant microbial communities present in the system were similar, including Pseudomonas, Chryseobacterium, Comamonas, Glutamicibacter, and Aeromonas. These microorganisms synergistically promoted the water purification function of the constructed wetland system. The contribution of stem microorganisms to the nitrate nitrogen removal in surface flow constructed wetlands was approximately 0.2 to 1.0 mg/g fresh weight, and reached its maximum value when the retention time was 70 hours.
- surface flow constructed wetlands,
- wetland plants,
- stem microorganisms,
- nitrogen removal,
- microbial diversity

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References(17)

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