Research advances in carbon source utilization and metabolic characteristics of polyphosphate-accumulating organisms （PAOs） in enhanced biological phosphorus removal systems

ZHENG Qianyun; XU Ketong; HE Jitao; CHE Qirui; ZHENG Yihua; ZHENG Haixin; YUAN Jing; CHEN Hang; CHEN Liping; WEI Chaohai; QIU Guanglei

doi:10.13205/j.hjgc.202503003

Volume 43 Issue 3

Mar. 2025

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2025 > 43(3): 22-41

ZHENG Qianyun, XU Ketong, HE Jitao, CHE Qirui, ZHENG Yihua, ZHENG Haixin, YUAN Jing, CHEN Hang, CHEN Liping, WEI Chaohai, QIU Guanglei. Research advances in carbon source utilization and metabolic characteristics of polyphosphate-accumulating organisms （PAOs） in enhanced biological phosphorus removal systems[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(3): 22-41. doi: 10.13205/j.hjgc.202503003

Citation:

ZHENG Qianyun, XU Ketong, HE Jitao, CHE Qirui, ZHENG Yihua, ZHENG Haixin, YUAN Jing, CHEN Hang, CHEN Liping, WEI Chaohai, QIU Guanglei. Research advances in carbon source utilization and metabolic characteristics of polyphosphate-accumulating organisms （PAOs） in enhanced biological phosphorus removal systems[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(3): 22-41. doi: 10.13205/j.hjgc.202503003

Citation:

ZHENG Qianyun, XU Ketong, HE Jitao, CHE Qirui, ZHENG Yihua, ZHENG Haixin, YUAN Jing, CHEN Hang, CHEN Liping, WEI Chaohai, QIU Guanglei. Research advances in carbon source utilization and metabolic characteristics of polyphosphate-accumulating organisms （PAOs） in enhanced biological phosphorus removal systems[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(3): 22-41. doi: 10.13205/j.hjgc.202503003

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Research advances in carbon source utilization and metabolic characteristics of polyphosphate-accumulating organisms （PAOs） in enhanced biological phosphorus removal systems

doi: 10.13205/j.hjgc.202503003

1. School of Environment and Energy, South China University of Technology, Guangzhou 510006, China;
2. Key Laboratory of Pollution Control and Ecological Restoration in Industrial Clusters, Ministry of Education, Guangzhou 510006, China;
3. Guangdong Provincial Key Laboratory of Solid Wastes Pollution Control and Recycling, Guangzhou 510006, China;
4. National Joint Research Center for Ecological Conservation and High Quality Development of the Yellow River Basin, Beijing 100012, China

Received Date: 2025-01-14
Accepted Date: 2025-02-26
Rev Recd Date: 2025-02-23

Available Online: 2025-06-07

Publish Date: 2025-03-01

Abstract

Abstract

Polyphosphate-accumulating organisms （PAOs） are key functional microorganisms in enhanced biological phosphorus removal （EBPR） systems， which take up carbon sources under anaerobic conditions and remove phosphorus under aerobic conditions. In recent years， with the increasing numbers of research on EBPR and PAOs， it has been found that different PAOs have different carbon source preferences and metabolic characteristics. This paper provides an overview of typical PAOs， including Candidatus Accumulibacter， Dechromomonas， Tetrasphaera， and Microlunatus phosphovorus， with a focus on their preferences and metabolic mechanisms on acetate， amino acids， and carbohydrates. The interactions between PAOs and glycogen-accumulating organisms （GAOs）， and different PAOs mediated by different carbon sources were also introduced and discussed. The application of specific carbon sources or alternating carbon sources， or the control and regulation of the carbon source supply rate based on the major differences in the carbon uptake bioenergetics of PAOs and GAOs are effective measures for the suppression of GAOs in the EPBR system. The synergetic mechanism of multiple carbon sources shown for Candidatus Accumulibacter lays a basis for carbon emission reduction in EBPR systems. The competition between Candidatus Accumulibacter and Tetrasphaera is inevitable when amino acids are used as carbon sources. Their interactions may further depend on the fermentation products generated by Tetrasphaera during the fermentation process. The interactions between Tetrasphaera and Microlunatus phosphorus with glucose or amino acids as carbon sources are yet to be further understood. Future research is needed to determine whether carbohydrates are an effective and beneficial carbon source for EBPR systems.
- polyphosphate accumulating organisms,
- Candidatus Accumulibacter,
- Dechloromonas,
- Microlunatus phosphovorus,
- Tetrasphaera,
- carbon source

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References(119)

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