Bacterial and archaeal diversity across communities within a freshwater Aiken Spring on the Qinghai-Tibet Plateau

HENG Shan; QIAO Lijuan; WANG Rong; HAN Rui; XING Jiangwa; ZHU Derui; SHEN Guoping

doi:10.13205/j.hjgc.202507018

Volume 43 Issue 7

Jul. 2025

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HENG Shan, QIAO Lijuan, WANG Rong, HAN Rui, XING Jiangwa, ZHU Derui, SHEN Guoping. Bacterial and archaeal diversity across communities within a freshwater Aiken Spring on the Qinghai-Tibet Plateau[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 167-175. doi: 10.13205/j.hjgc.202507018

Citation:

HENG Shan, QIAO Lijuan, WANG Rong, HAN Rui, XING Jiangwa, ZHU Derui, SHEN Guoping. Bacterial and archaeal diversity across communities within a freshwater Aiken Spring on the Qinghai-Tibet Plateau[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 167-175. doi: 10.13205/j.hjgc.202507018

Citation:

HENG Shan, QIAO Lijuan, WANG Rong, HAN Rui, XING Jiangwa, ZHU Derui, SHEN Guoping. Bacterial and archaeal diversity across communities within a freshwater Aiken Spring on the Qinghai-Tibet Plateau[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 167-175. doi: 10.13205/j.hjgc.202507018

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Bacterial and archaeal diversity across communities within a freshwater Aiken Spring on the Qinghai-Tibet Plateau

doi: 10.13205/j.hjgc.202507018

1. Department of Basic Medical Sciences, Medical College, Qinghai Unversity, Xining 810016, China;
2. Academy of Agriculture and Forestry, Qinghai University, Xining 810016, China

Received Date: 2024-09-12
Accepted Date: 2024-12-01
Rev Recd Date: 2024-10-20

Available Online: 2025-09-11

Abstract

Abstract

Aiken Spring （AKS） is the deepest fresh water spring in the western reaches of the Qinghai-Tibet Plateau. The water body is rich in a variety of toxic heavy metals， but community structure of the bacteria and archaea involved is still unclear. To explore the diversity of bacteria and archaea in spring water， the differences in community composition， and the relationship between dominant genera and environmental factors， as well as to identify the available potential bacterial species， the bacterial V₃ to V₄ region and the archaeal V₃ to V₅ region of the 16S rRNA gene from AKS were sequenced using the high-throughput Illumina platform. According to a 97% similarity level， operational taxonomic units （OTUs） were screened and analyzed based on the phylum， class， and genus levels. Multivariate direct gradient regression was used to analyze the correlation among samples， dominant species， and environmental factors. A total of 19 phyla， 32 classes， 195 genera （491 OTUs） of bacteria， and 6 phyla， 8 classes， 56 genera （787 OTUs） of archaea were identified. The dominant bacterial genera were Roseovarius （8.59% to 26.84%）， Pseudorhodobacter （1.22% to 22.94%）， and Rehaibacterium （0.19% to 7.28%）. The dominant archaeal genera were Halolamina （6.29% to 21.17%）， Halohasta （10.93% to 15.89%）， and Halobaculum （7.69% to 11.82%）. The dominant bacterial genera Novosphingobium， Rehaibacterium， and Geminocystis were positively correlated with environment factors including total phosphorus， SO₄^2-， Mg²⁺， pH，and temperature. The dominant genera Rhodoferax， Porphyrobacter， Methylotenera， and Rubrimonas were significantly positively correlated with Na⁺， Ca²⁺， Cl^-， and total basicity. The Shannon diversity index （2.10 to 3.43） of bacteria in AKS was significantly higher than that of archaea （2.14 to 2.52）. Compared with other freshwater springs， the dominant populations of AKS show obvious differences and are greatly affected by environmental factors such as temperature， pH， SO₄^2-， and Mg²⁺， which provides an important theoretical reference for the subsequent development and utilization of microbial resources in the spring.
- Aiken Spring,
- bacteria,
- archaea,
- high-throughput sequencing,
- diversity

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References(30)

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