Lactic acid production from distiller's grains via simultaneous saccharification and fermentation using <i>Lactobacillus casei</i>

WANG Wanqing; WANG Nuohan; ZHENG Jin; WU Hongyu; LI Yongsheng; WANG Qunhui

doi:10.13205/j.hjgc.202507019

Volume 43 Issue 7

Jul. 2025

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2025 > 43(7): 176-183

WANG Wanqing, WANG Nuohan, ZHENG Jin, WU Hongyu, LI Yongsheng, WANG Qunhui. Lactic acid production from distiller's grains via simultaneous saccharification and fermentation using Lactobacillus casei[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 176-183. doi: 10.13205/j.hjgc.202507019

Citation:

WANG Wanqing, WANG Nuohan, ZHENG Jin, WU Hongyu, LI Yongsheng, WANG Qunhui. Lactic acid production from distiller's grains via simultaneous saccharification and fermentation using Lactobacillus casei[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 176-183. doi: 10.13205/j.hjgc.202507019

Citation:

WANG Wanqing, WANG Nuohan, ZHENG Jin, WU Hongyu, LI Yongsheng, WANG Qunhui. Lactic acid production from distiller's grains via simultaneous saccharification and fermentation using Lactobacillus casei[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(7): 176-183. doi: 10.13205/j.hjgc.202507019

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Lactic acid production from distiller's grains via simultaneous saccharification and fermentation using Lactobacillus casei

doi: 10.13205/j.hjgc.202507019

1. Department of Environmental Engineering, Tianjin College, University of Science and Technology Beijing, Tianjin 301830, China;
2. Department of Environmental Science and Engineering, College of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China;
3. China Petroleum Group Safety and Environmental Protection Technology Research Institute Company Limited, Beijing 100083, China

Received Date: 2025-05-02
Accepted Date: 2025-07-03
Rev Recd Date: 2025-06-20

Available Online: 2025-09-11

Abstract

Abstract

To achieve high-value utilization of distiller's grains waste by producing lactic acid through biological fermentation during the brewing process， this study investigated the proliferation and acid production capacity of the employed lactic acid bacteria at different temperatures. It compared the effects of simultaneous saccharification and fermentation （SSF） versus separate hydrolysis and fermentation （SHF） on lactic acid production under varying temperature conditions， and optimized the SSF working parameters. Results showed that Lactobacillus casei exhibited delayed growth at 50 ℃ compared to 35 ℃， but its lactic acid production surged after 48 hours of cultivation， approaching the levels observed under 35 ℃. Compared to SHF， SSF at 50 ℃ yielded 10.1% higher lactic acid output， reduced fermentation time by 72 hours， and shortened the period for lactic acid bacteria to dominate the microbial community （approaching total bacterial counts） by 48 hours. When SSF was conducted with pH adjusted to 6.0， at a 24-hour interval using ammonia water and Lactobacillus casei inoculation at 2.1% （by weight ratio）， 1 gram of fresh distiller's grains yielded 98 mg of lactic acid. This study demonstrates the feasibility of SSF for lactic acid production from distiller's grains at elevated temperatures （50 ℃）， possessing advantages such as higher fermentation efficiency， simplified operational processes， reduced equipment investment， and lower production costs. These findings provide novel insights and technical support for the circular utilization of distiller's grains resources.
- distiller's grains,
- SSF,
- SHF,
- Lactobacillus casei,
- lactic acid

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References(21)

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