Effects of preservation method and inoculum-to-substrate ratio on anaerobic digestion of food waste and analysis of carbon reduction potential

ZHAO Pan; REN Yuanyuan; LIU Junjie; ZHANG Shuang; WANG Qunhui

doi:10.13205/j.hjgc.202510006

Volume 43 Issue 10

Oct. 2025

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2025 > 43(10): 46-53

ZHAO Pan, REN Yuanyuan, LIU Junjie, ZHANG Shuang, WANG Qunhui. Effects of preservation method and inoculum-to-substrate ratio on anaerobic digestion of food waste and analysis of carbon reduction potential[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 46-53. doi: 10.13205/j.hjgc.202510006

Citation:

ZHAO Pan, REN Yuanyuan, LIU Junjie, ZHANG Shuang, WANG Qunhui. Effects of preservation method and inoculum-to-substrate ratio on anaerobic digestion of food waste and analysis of carbon reduction potential[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 46-53. doi: 10.13205/j.hjgc.202510006

Citation:

ZHAO Pan, REN Yuanyuan, LIU Junjie, ZHANG Shuang, WANG Qunhui. Effects of preservation method and inoculum-to-substrate ratio on anaerobic digestion of food waste and analysis of carbon reduction potential[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 46-53. doi: 10.13205/j.hjgc.202510006

PDF( 763 KB)

Effects of preservation method and inoculum-to-substrate ratio on anaerobic digestion of food waste and analysis of carbon reduction potential

doi: 10.13205/j.hjgc.202510006

1. Department of Environmental Engineering, Tianjin College, University of Science and Technology Beijing, Tianjin 301830, China;
2. Department of Environmental Science and Engineering, School of Energy and Environmental Engineering, University of Science and Technology Beijing, Beijing 100083, China

Received Date: 2024-11-03
Accepted Date: 2024-12-21
Rev Recd Date: 2024-12-01

Available Online: 2025-12-03

Publish Date: 2025-10-01

Abstract

Abstract

Anaerobic digestion （AD）， with the advantages of being able to utilize complex substrates and the target products can be easily separated， is one of the mainstream technologies for the resource utilization of food waste （FW）. AD is of great significance for energy conservation and emission reduction and achieving the Dual Carbon Goals. However， the growth of pathogenic bacteria and the generation of odors during the transportation of FW may threaten public health security and affect the efficiency of AD. Based on the fact that lactic acid produced during anaerobic preservation of FW has antibacterial and deodorizing effects， this study first explored the upper limit of the allowable concentration of lactic acid in the AD process of FW through simulation experiments， and then the methane fermentation and carbon reduction potential of FW after anaerobic preservation or natural preservation as a substrate under different inoculum-to-substrate ratio （R_I/S=0.5∶1 and R_I/S=1∶1） were studied. The results showed that when the lactic acid concentration produced during the anaerobic preservation of FW did not exceed 15 g/L， it did not inhibit methane fermentation； instead， it had a promoting effect. Under the same preservation method， the gas production efficiency at R_I/S of 0.5∶1 was significantly lower than that at R_I/S of 1∶1. However， at the two inoculation ratios， the anaerobic preservation groups all exhibited higher gas production efficiency compared to the natural preservation groups. The results of the stability analysis showed that when R_I/Swas 1∶1， the system had a higher alkalinity and pH， which was less prone to acidification， and had good stability. The results of carbon accounting analysis showed that AD of FW after anaerobic preservation can effectively promote the carbon neutrality process. This study provides valuable practical guidance for the engineering application of food waste anaerobic digestion.
- food waste,
- anaerobic preservation,
- inoculation ratio,
- anaerobic digestion,
- carbon accounting

FullText(HTML)

References(20)

References

[1]	JIA N，JIAO J，WANG S，et al. Research progress on the resource utilization and high-value utilization technologies of food waste［J］. Heilongjiang Environmental Journal，2025，38（2）:1-4. 贾楠，焦建会，王圣惠，等. 餐厨垃圾资源化和高值化利用技术研究进展［J］. 黑龙江环境通报，2025，38（2）:1-4.
[2]	YANG M X，SHEN P F，CHEN X H. Analysis and suggestion of product-oriented anaerobic fermentation of kitchen waste.［J］. Environmental Engineering，2024，42（10）:140-146 杨梦霞，沈鹏飞，陈小欢. 以产物为导向的厨余垃圾厌氧发酵方式分析与建议［J］. 环境工程，2024，42（10）:140-146.
[3]	YUAN T，SHI X，SUN R，et al. Simultaneous addition of biochar and zero-valent iron to improve food waste anaerobic digestion［J］. Journal of Cleaner Production，2021，278:123627
[4]	LI J N，WANG Q H，LIANG B R，et al. Effects of garden waste on emission reduction and microbial Community in coastal saline soil.［J］. Environmental Engineering，2024，42（1）:95-101.. 李婧男，汪群慧，梁宝瑞，等. 园林废弃物对滨海盐渍土固碳减排效应与微生物群落结构的影响［J］. 环境工程，2024，42（1）:95-101
[5]	YANG K，ZHOU Y，ZHOU Y，et al. Experimental study on methane deflagration suppression by ultrafine water mist containing PPFBS［J］. Safety and Environmental Engineering，2020，27（6）:174-180. 杨克，周越，周扬，等. 含PPFBS超细水雾抑制甲烷爆燃的实验研究［J］. 安全与环境工程，2020，27（6）:174-180.
[6]	YUE L C，YU M，CHENG J，et al. Influence of lipid content and electric fermentation voltage on methane production from food waste anaerobic digestion.［J］. Environmental Engineering，2024，42（7）:200-207. 岳良辰，俞苗，程军，等. 油脂含量和电发酵电压对含油餐厨垃圾厌氧消化产甲烷的影响［J］. 环境工程，2024，42（7）:200-207.
[7]	HAO X D，WANG X Y，CAO D Q，et al. Analysis of CO₂ emission from fossil carbon in organics from wastewater［J］. CHINA WATER & WASTEWATER，2018，34（2）:13-17 郝晓地，王向阳，曹达啟，等. 污水有机物中化石碳排放CO₂辨析［J］. 中国给水排水，2018，34（2）:13-17.
[8]	ZHENG X W，LI B，GUO D，et al. Start-up performance and methane production efficiency on anaerobic digestion for kitchen waste［J］. Environmental Engineering，2018，36（9）:128-132. 郑晓伟，李兵，郭栋，等. 餐厨垃圾厌氧发酵启动特性与产甲烷效率［J］. 环境工程，2018，36（9）:128-132.
[9]	LI W Z，XU M H，LUO L N. Effect of different inoculum concentrations on anaerobic fermentation characteristics from rice straw［J］. Journal of Northeast Agricultural University，2012，43（11）:55-60. 李文哲，徐名汉，罗立娜. 不同接种量对稻秆厌氧发酵特性的影响［J］. 东北农业大学学报，2012，43（11）:55-60.
[10]	XU H，GAO Y，LI X. Effects of total solids content and inoculation ratio on high solid anaerobic digestion of food waste［J］. Renewable Energy Resources，2022，40（8）:1020-1027. 许恒彬，高原，李秀金. 含固率与接种比对分类厨余垃圾高固厌氧消化性能的影响［J］. 可再生能源，2022，40（8）:1020-1027.
[11]	ZHEN Y Y，GE Y H，SHI G Z，et al. Effects of total solids content and inoculation ratio on anaerobic digestion of vegetable waste［J］. China Biogas，2020，38（2）:45-51. 甄月月，葛一洪，施国中，等. 不同含固率和接种比对尾菜厌氧消化的影响［J］. 中国沼气，2020，38（2）:45-51.
[12]	National Environmental Protection Administration. Monitoring and analysis methods for water and wastewater［M］. 4 th Edition. Beijing:China Environmental Press，2002. 国家环境保护总局. 水和废水监测分析方法［M］. 4版. 北京:中国环境科学出版社，2002.
[13]	ZHEN G，LU X，KOBAYASHI T，et al. Mesophilic anaerobic co-digestion of waste activated sludge and Egeria densa:Performance assessment and kinetic analysis［J］. Applied Energy，2015，148:78-86.
[14]	KAFLE G K，KIM S H，SUNG K I. Ensiling of fish industry waste for biogas production:A lab scale evaluation of biochemical methane potential（BMP）and kinetics［J］. Bioresource Technology，2013，127:326-336.
[15]	XIA X，SHAO Q，CAO Y，et al. Analysis of energy recovery and carbon emission during sludge Anaerobic digestion under different treatment routes［J］. Environmental Engineering，2023，41（7）:1-7，13. 夏雪，邵钱祺，曹悦，等. 不同处理模式下污泥厌氧消化的能源回收与碳排放分析［J］. 环境工程，2023，41（7）:1-7，13.
[16]	BISWAL B K，HUANG H，DAI J，et al. Impact of low-thermal pretreatment on physicochemical properties of saline waste activated sludge，hydrolysis of organics and methane yield in anaerobic digestion［J］. Bioresource Technology，2019，297:122423.
[17]	ZHAO M，LI X，LI L，et al. Effects of alkalinity type and concentration on mesophilic anaerobic digestion of excess sludge［J］. China Environmental Science，2019，39（5）:1954-1960. 赵明明，李夕耀，李璐凯，等. 碱度类型及浓度对剩余污泥中温厌氧消化的影响［J］. 中国环境科学，2019，39（5）:1954-1960.
[18]	LIU Z，ZHANG C，WANG L，et al. Effects of furan derivatives on biohydrogen fermentation from wet steam-exploded cornstalk and its microbial community［J］. Bioresource Technology，2015，175:152-159.
[19]	JANG H M，HA J H，KIM M S，et al. Effect of increased load of high-strength food wastewater in thermophilic and mesophilic anaerobic co-digestion of waste activated sludge on bacterial community structure［J］. Water Research，2016，99:140-148.
[20]	WU C，HUANG Q，YU M，et al. Effects of digestate recirculation on a two-stage anaerobic digestion system，particularly focusing on metabolite correlation analysis［J］. Bioresource Technology，2018，251:40-48.