Analysis of planktonic community succession and algal bloom causes in campus landscape waterbodies supplied by reclaimed water

LI Jun; WU Yinhu; LIANG Siyi; CHEN Zhuo; LI Jiankang; ZHAO Lu; HU Hongying

doi:10.13205/j.hjgc.202510010

Volume 43 Issue 10

Oct. 2025

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LI Jun, WU Yinhu, LIANG Siyi, CHEN Zhuo, LI Jiankang, ZHAO Lu, HU Hongying. Analysis of planktonic community succession and algal bloom causes in campus landscape waterbodies supplied by reclaimed water[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 84-94. doi: 10.13205/j.hjgc.202510010

Citation:

LI Jun, WU Yinhu, LIANG Siyi, CHEN Zhuo, LI Jiankang, ZHAO Lu, HU Hongying. Analysis of planktonic community succession and algal bloom causes in campus landscape waterbodies supplied by reclaimed water[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 84-94. doi: 10.13205/j.hjgc.202510010

Citation:

LI Jun, WU Yinhu, LIANG Siyi, CHEN Zhuo, LI Jiankang, ZHAO Lu, HU Hongying. Analysis of planktonic community succession and algal bloom causes in campus landscape waterbodies supplied by reclaimed water[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(10): 84-94. doi: 10.13205/j.hjgc.202510010

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Analysis of planktonic community succession and algal bloom causes in campus landscape waterbodies supplied by reclaimed water

doi: 10.13205/j.hjgc.202510010

1. State Key Laboratory of Regional Environmental Safety, State Environmental Protection Key Laboratory of Microorganism Application and Risk Control (MARC), School of Environment, Tsinghua University, Beijing 100084, China;
2. MCC Capital Engineering & Research Incorporation Limited, Beijing 100176, China;
3. College of Ocean Engineering, Rizhao Polytechnic, Rizhao 276800, China

Received Date: 2024-12-27
Accepted Date: 2025-05-07
Rev Recd Date: 2025-05-01

Available Online: 2025-12-03

Publish Date: 2025-10-01

Abstract

Abstract

Aquatic landscapes play a vital role in urban ecosystems. In northern China， where water resources are severely scarce， reclaimed water has emerged as a primary solution for replenishing these landscapes. However， residual nutrients and organic matter in reclaimed water can easily cause water quality fluctuations and increase the risk of algal blooms. In this paper， a lotus pond at a university was selected as a typical reclaimed water landscape to investigate seasonal variations in water quality and planktonic microbial communities. Through analyzing the seasonal variations in water quality and planktonic microbial communities in the lotus pond， it was found that concentrations of chemical oxygen demand （COD）， total nitrogen （TN）， total phosphorus （TP）， and suspended solids （SS） were all significantly higher in autumn than in spring. However， these water quality indicators alone could not directly explain the occurrence of algal blooms. Instead， the temperature increase in April emerged as the primary driver， significantly promoting the rapid growth of Cryptophyta， making it the primary dominant algal group during blooms. High-throughput sequencing of microbial communities further demonstrated pronounced seasonal differences in species richness and diversity. The microbial community diversity in autumn was higher than in spring， while the community structure during the pre-bloom period （March） and the bloom period （April） in spring showed a high degree of consistency. Cryptophyta and Chlorophyta were the primary dominant algae in the lotus pond， exhibiting an alternating abundance pattern in response to temperature variations. Spring temperature increase significantly accelerated the growth of Cryptophyta， while Chlorophyta showed greater sensitivity to temperature fluctuations， with notable abundance variations observed in autumn. Environmental factor analysis using canonical correspondence analysis （CCA） highlighted that temperature， COD， TN， and SS played critical roles in shaping microbial community structures. In autumn， declining temperatures and nutrient accumulation favored nutrient-dependent microbial communities while suppressing phytoplankton growth. In contrast， rising temperatures in spring accelerated algal proliferation， significantly altering community structures during the April bloom. This study provides a scientific basis for water quality management and microbial community regulation in reclaimed water landscapes， offering theoretical and practical insights for improving the ecological health of aquatic landscapes and developing effective algal bloom control strategies.
- campus landscapes,
- reclaimed water,
- algal blooms,
- biodiversity,
- seasonal changes,
- environmental factors

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References(49)

References

[1]	BA W，WANG D，GONG B，et al. Urban water scarcity in China:A systematic review of research advances and future directions［J］. Applied Geography，2023，159:103069.
[2]	LUO Y L，PAN Y R，MA J X，et al. Research advances on carbon emission of wastewater resource recovery and valorization［J］. Environmental Engineering，2022，40（6）:83-91. 罗雨莉，潘艺蓉，马嘉欣，等. 污水再生与增值利用的碳排放研究进展［J］. 环境工程，2022，40（6）:83-91.
[3]	LIU J P. The causes and solutions of water crisis in China［J］. Environmental Engineering，2008，26（4）:97-99，6. 刘继平. 关于我国水危机的成因分析与对策建议［J］. 环境工程，2008，26（4）:97-99，6.
[4]	HU H Y. Development report on municipal wastewater treatment and reuse in China［M］. Beijing:China Architecture and Building Press，2021. 胡洪营. 中国城镇污水处理与再生利用发展报告［M］. 北京:中国建筑工业出版社，2021.
[5]	WANG H L，CHEN L，LIANG W G，et al. Research on strategy and technique of water quality standard for reuse of reclaimed water in scenic water［J］. Journal of Environmental Science，2002，23（S1）:93-98. 王鹤立，陈雷，梁伟刚，等. 再生水回用于人工景观水体的水质目标:策略与技术［J］. 环境科学，2002，23（增刊1）:93-98.
[6]	FENG C M，LI Y，ZHANG Y J，et al. Eutrophication of enclosed landscape water supplemented by reclaimed water［J］. Journal of Environmental Sciences，2010，43（8）:727-732. 冯萃敏，李莹，张雅君，等. 以再生水为水源的封闭景观水体营养状态分析［J］. 天津大学学报，2010，43（8）:727-732.
[7]	SHAN X，LI C G，LI F M. Water quality variation of a typical urban landscape river replenished with reclaimed water［J］. Water Cycle，2023，4:137-144.
[8]	CHANG N，ZHANG Q，WANG Q，et al. Current status and characteristics of urban landscape lakes in China［J］. Science of the Total Environment，2020，712:135669.
[9]	SONG K，LU Y，DAO G，et al. Reclaimed water for landscape water replenishment:Threshold nitrogen and phosphorus concentrations values for bloom control［J］. Algal Research，2022，62:102608.
[10]	SONG K，LIU Q，WANG Q，et al. Replenishment of landscape water with reclaimed water:threshold of hydraulic retention time employing transparency as a control indicator［J］. Water Reuse，2024，14（2）:240-247.
[11]	LIU F，FENG M Q，WANG Y B. Microbial community structure of estuary of the Fenhe River into the Yellow River in summer［J］. Microbiology China，2019，46（1）:54-56. 刘峰，冯民权，王毅博. 汾河入黄口夏季微生物群落结构分析［J］. 微生物学通报，2019，46（1）:54-56.
[12]	YU Z D，OUYANG Z Y，ZHENG C Y，et al. Multivariate environmental factors and seasonal spatial dynamics affecting the phytoplankton community in Yazhou Bay，South China Sea［J］. Water，2024，16（22）:16.
[13]	XU C，MA Y，ZHANG H，et al. The impact of environmental factors on the spatiotemporal heterogeneity of phytoplankton community structure and biodiversity in the Qiongzhou Strait［J］. Water，2023，15（21）:3792.
[14]	YOON J N，LEE M，JIN H，et al. Summer distributional characteristics of surface phytoplankton related with multiple environmental variables in the Korean coastal waters［J］. Journal of Marine Science and Engineering，2022，10（7）:850.
[15]	XU S，XIAO Y，XU Y，et al. Effects of seasonal variations and environmental factors on phytoplankton community structure and abundance in Beibu Gulf，China［J］. Ocean and Coastal Management，2024，248:106982.
[16]	GENG Y，LI M，YU R，et al. Response of planktonic diversity and stability to environmental drivers in a shallow eutrophic lake［J］. Ecological Indicators，2022，144:109560.
[17]	State Environmental Protection Administration（SEPA），Editorial Committee of Water and Wastewater Monitoring and Analysis Methods.（2002）. Water and wastewater monitoring and analysis methods［M］.（4th Ed.）. Beijing:China Environmental Science Press，2002. 国家环境保护总局《水和废水监测分析方法》编委会. 水和废水监测分析方法［M］. 4版. 北京:中国环境科学出版社，2002.
[18]	SCHLOSS P D，WESTCOTT S L，RYABIN T，et al. Introducing mothur:Open-source，platform-independent，community-supported software for describing and comparing microbial communities［J］. Applied and Environmental Microbiology，2009，75:7537.
[19]	SEGATA N，IZARD J，WALDRON L，et al. Metagenomic biomarker discovery and explanation［J］. Genome Biology，2011，12（6）:18.
[20]	National Technical Committee on Urban Water Supply and Drainage of Standardization Administration of China. Reuse of urban recycling water-water quality standard for scenic environment use:GB/T 18921—2019［S］. Beijing:Standards Press of China，2019. 全国城镇给水排水标准化技术委员会. 城市污水再生利用景观环境用水水质:GB/T 18921—2019［S］. 北京:中国标准出版社，2019.
[21]	Ministry of Ecology and Environment（MEE）and State Administration for Market Regulation（SAMR）. Environmental quality standards for surface water:GB 3838—2002［S］. Beijing:Standards Press of China，2002. 国家市场监督管理总局，国家标准化管理委员会. 地表水环境质量标准:GB 3838—2002［S］. 北京:中国标准出版社，2002.
[22]	YANG H Y，ZHANG Y L，LI N，et al. Quantifying the effect of wind on cyanobacteria blooms based on high frequency hyper-spectral proximity observations［J］. Journal of Lake Sciences，2023，35（6）:1927-1938. 杨华音，张运林，李娜，等. 基于高频高光谱近感观测量化风对蓝藻水华的影响［J］. 湖泊科学，2023，35（6）:1927-1938.
[23]	SINGH S P，SINGH P. Effect of temperature and light on the growth of algae species:a review［J］. Renewable and Sustainable Energy Reviews，2015，50:431-444.
[24]	ZHANG H，XU Y，LIU X，et al. Different seasonal dynamics，ecological drivers，and assembly mechanisms of algae in southern and northern drinking water reservoirs［J］. Science of the Total Environment，2024，922:171285.
[25]	GUO Y Q，MENG H，DENG D L，al er. Research process on effects of climate warming on the growth，metabolism and mechanism of Microcystis［J］. Environmental Ecology，2023，5（7）:79-86. 郭咏琪，孟晗，邓代兰，等. 气候变暖对微囊藻的生长、代谢及机制研究进展［J］. 环境生态学，2023，5（7）:79-86.
[26]	DONG J，GAO Y N，LI B G. A review:Responses of phytoplankton communities to eutrophication and climate warming in freshwater lakes［J］. Acta Hydrobiologica Sinica，2016，40（3）:615-623. 董静，高云霓，李根保. 淡水湖泊浮游藻类对富营养化和气候变暖的响应［J］. 水生生物学报，2016，40（3）:615-623.
[27]	WANG J Q，SONG Y Z，HUANG J. Effects of increasing water temperature on water properties and aquatic organisms:A critical review［J］. Journal of Hydroecology，2020，41（1）:100-109. 王锦旗，宋玉芝，黄进. 水温升高对水体性质及水生生物的影响研究进展［J］. 水生态学杂志，2020，41（1）:100-109.
[28]	ZHANG H，ZHAO Y，YIN H，et al. Effect of aquatic macrophyte growth on landscape water quality improvement［J］. Environmental Science and Pollution Research，2019，26（33）:33791-33803.
[29]	YANYAN D，YAO C，CHUNLONG L I. Purification efficiency of different aquatic plants in eutrophic water［J］. Environmental Science and Technology，2015，38（8）:223-227.
[30]	LAVERGNE C，BOVIO-WINKLER P，ETCHEBEHERE C，et al. Towards centralized biogas plants:Co-digestion of sewage sludge and pig manure maintains process performance and active microbiome diversity［J］. Bioresource Technology，2020，297:122442.
[31]	YUAN X C，WANG M，GUO X Y，et al. v Analysis of the seasonal changes in planktonic microbial diversity in urban river supplied with reclaimed water:a case study of the North Canal River［J］. Environmental Science，2022，43（8）:4097-4107. 袁训超，王敏，郭逍宇，等. 再生水河道浮游微生物多样性季节变化分析:以北运河为例［J］. 环境科学，2022，43（8）:4097-4107.
[32]	LIU H X，HUANG H H，XU S N，et al. Planktonic community structure during a harmful bloom of Phaeocystis globosa in a subtropical bay，with special reference to the ciliate assemblages［J］. Ecotoxicology，2015，24（7）:1419-1429.
[33]	ZHANG H，HOU F，XIE W，et al. Interaction and assembly processes of abundant and rare microbial communities during a diatom bloom process［J］. Environmental Microbiology，2019，22（5）:1707-1719.
[34]	LI J，SUN Y J，YIN M，et al. Seasonal variations in microbial community structure in Yongding River［J］. Journal of Beijing Normal University（Natural Science Edition），2020，56（2）:257-264. 李洁，孙寓姣，尹萌，等. 永定河山峡与城市段微生物群落结构季节变化［J］. 北京师范大学学报（自然科学版），2020，56（2）:257-264.
[35]	GUO Z Z，SU Z H，DI Y M，et al. Analysis on diversity of plankton microbial community in the Beijing-Tianjin-Hebei section of the North Canal River［J］. Environmental Science，2022，43（2）:803-812. 郭芝芝，苏振华，邸琰茗，等. 北运河京津冀段河道浮游微生物群落多样性变化分析［J］. 环境科学，2022，43（2）:803-812.
[36]	BARWELL L J，ISAAC N J B，KUNIN W E. Measuring β-diversity with species abundance data［J］. Journal of Animal Ecology，2015，84（4）:1112-1122.
[37]	ODUM E P. The strategy of ecosystem development［J］. Science，1969，164（3877）:262-270.
[38]	ZHOU L，XIE S G，HUANG Y. Molecular techniques-based microbial ecology in natural waters［J］. Environmental Science and Technology，2010，33（7）:85-89，101. 邹莉，谢曙光，黄艺. 天然水体基于分子生物学的微生物生态学研究［J］. 环境科学与技术，2010，33（7）:85-89，101.
[39]	LI Z，SHU-GUANG X，YI H. Molecular techniques-based microbial ecology in natural waters［J］. Environmental Science and Technology，2010，33:85-101.
[40]	GRUJCIC V，NUY J K，SALCHER M M，et al. Cryptophyta as major bacterivores in freshwater summer plankton［J］. The ISME Journal，2018，12（7）:1668-1681.
[41]	SOMMER U，GLIWICZ Z M，LAMPERT W，et al. The plankton ecology group model of seasonal succession of planktonic events in fresh waters［J］. Fundamental and Applied Limnology，1986，106（4）:244-471.
[42]	WANG W，WANG H，FENG Y，et al. Consistent responses of the microbial community structure to organic farming along the middle and lower reaches of the Yangtze River［J］. Scientific Reports，2016，6（1）:35046.
[43]	JOUSSET A，BIENHOLD C，CHATZINOTAS A，et al. Where less may be more:how the rare biosphere pulls ecosystems strings［J］. The ISME Journal，2017，11（4）:853-862.
[44]	CAMPBELL B J，YU L，HEIDELBERG J F，et al. Activity of abundant and rare bacteria in a coastal ocean［J］. Proceedings of the National Academy of Sciences，2011，108（31）:12776-12781.
[45]	NIU Y，YU H，JIANG X. Within-lake heterogeneity of environmental factors structuring bacterial community composition in Lake Dongting，China［J］. World Journal of Microbiology and Biotechnology，2015，31（11）:1683-1689.
[46]	SHANG Y，WU X，WANG X，et al. Factors affecting seasonal variation of microbial community structure in Hulun Lake，China［J］. Science of The Total Environment，2022，805:150294.
[47]	CHEN M，FAN M，LIU R，et al. The dynamics of temperature and light on the growth of phytoplankton［J］. Journal of Theoretical Biology，2015，385:8-19.
[48]	ZHENG X X，WANG M T，LIU X X，et al. Revealing assembly mechanisms of algal communities in aquatic microniches:Shifts in diversity patterns，microbial interactions and stability along nutrient gradients［J］. Environmental Research，2024，262（1）:119798.
[49]	FERNANDEZ-GONZALEZ C，TARRAN G A，SCHUBACK N，et al. Phytoplankton responses to changing temperature and nutrient availability are consistent across the tropical and subtropical Atlantic［J］. Communications Biology，2022，5:1035.