INFLUENCE OF PETROLEUM CONTAMINATION ON SOIL MICROBIAL COMMUNITY AND ISOLATION AND IDENTIFICATION OF OIL-DEGRADING BACTERIA

LIU Xiaodong; YU Tianfei; AI Jiamin; LI Jing; ZHANG Baobao; JIANG Yingying; DENG Zhenshan

doi:DOI:10.13205/j.hjgc.202207009

Volume 40 Issue 7

Sep. 2022

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2022 > 40(7): 61-68

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LIU Xiaodong, YU Tianfei, AI Jiamin, LI Jing, ZHANG Baobao, JIANG Yingying, DENG Zhenshan. INFLUENCE OF PETROLEUM CONTAMINATION ON SOIL MICROBIAL COMMUNITY AND ISOLATION AND IDENTIFICATION OF OIL-DEGRADING BACTERIA[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(7): 61-68. doi: DOI:10.13205/j.hjgc.202207009

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INFLUENCE OF PETROLEUM CONTAMINATION ON SOIL MICROBIAL COMMUNITY AND ISOLATION AND IDENTIFICATION OF OIL-DEGRADING BACTERIA

doi: DOI:10.13205/j.hjgc.202207009

College of Life Sciences, Yan'an University, Yan'an 716000, China

Received Date: 2021-07-07
Available Online: 2022-09-02

Abstract

Abstract

This research intended to investigate the influence of oil contamination on bacterial community,soil physico-chemical properties,and to isolate and identify oil-degrading bacteria.In this work,the microbial community structure of oil-contaminated soil and control soil in Baota,Wuqi,Jingbian,and Yanchang counties in northern Shaanxi were analyzed based on high-throughput sequencing technology,and the oil content,organic matter,nitrate nitrogen,ammonium nitrogen,available phosphorus,available potassium and pH were measured.With petroleum as the sole carbon source,oil-degrading bacteria were screened from oil-contaminated soils and identified based on 16S rDNA gene sequence.The contents of ammonium nitrogen,nitrate nitrogen,available phosphorus,and available potassium decreased by 0.57,6.63,4.34,8.91 mg/kg,respectively,and the content organic matter increased by 2~21 times.The richness and diversity of microorganisms were reduced after being contaminated with crude oil.In the oil-contaminated soil,Proteobacteria and Chloroflexi were the dominant phylum,and Mycobacterium was the genus with the highest abundance.Using petroleum as the only carbon source,8 strains of oil-degrading bacteria were isolated,among them,the 5-day oil degradation rates of OS33 and OS62-1 strains reached 80.51% and 81.60%.Finally,OS33 was identified as Dietzia sp.,and OS62-1 was Rhodococcus sp.After being contaminated with crude oil,the soil microbial community richness and diversity were decreased.8 oil-degrading bacteria were isolated and the highest 5-day oil degradation rate was 81.60%.This study further enriched the oil-degrading bacteria resource of northern Shaanxi.
- petroleum pollution,
- soil,
- degrading,
- Rhodococcus sp.,
- Dietzia sp.

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References

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