1株海洋产电菌<i>Shewanella</i> XMS-1的特性分析

张宗斌; 岳正波; 吴景行; 王进

doi:10.13205/j.hjgc.202101004

1株海洋产电菌Shewanella XMS-1的特性分析

doi: 10.13205/j.hjgc.202101004

1. 合肥工业大学资源与环境工程学院, 合肥 230009;
2. 中国科学技术大学化学与材料科学学院, 合肥 230026

基金项目:

国家自然科学基金项目（41772361）。

详细信息

作者简介:
张宗斌(1995-),男,硕士研究生,主要研究方向为环境微生物,水污染控制。zhangzongbin@mail.hfut.edu.cn

通讯作者:
王进(1978-),女,博士,教授,主要研究方向为环境微生物,矿山环境治理,水污染控制。sophiawj@hfut.edu.cn

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- 文章访问数: 321
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出版历程
- 收稿日期: 2020-01-13
- 网络出版日期: 2021-04-23

CHARACTERISTICS ANALYSIS OF AN ELECTRICITY-PRODUCING STRAIN SHEWANELLA XMS-1 FROM MARINE SEDIMENTS

1. School of Resources and Environmental Engineering, Hefei University of Technology, Hefei 230009, China;
2. School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China

摘要

摘要: 以厦门湾海水中的底泥为接种物筛选得到1株具有电化学活性的菌株XMS-1，研究该菌株相关特性及其在环境污染控制中的应用。根据菌株的形态、生理生化性质及16S rRNA基因测序的结果确定其种属，并用以构建微生物燃料电池，研究该菌株的产电性能，以及对典型重金属污染物Cr （Ⅵ）的还原能力。菌株XMS-1经鉴定属于Shewanella属，当MFC中接种初始浓度OD₆₀₀为0.3时可获得最大功率密度18 mW/m²、电流密度243 mA/m²，细菌能够在pH为7~8和NaCl含量为0~6%条件下实现对Cr （Ⅵ）的还原。XMS-1该菌株能在较宽的pH和较高的盐度条件下高效还原Cr（Ⅵ），并且具备良好的电化学活性，为微生物直接处理含盐工业废水和实现海水资源化利用提供了新的研究思路和数据参考。
- 微生物燃料电池 /
- Cr(Ⅵ) /
- 废水处理 /
- 筛选 /
- 细菌
Abstract: An electricity-producing strain XMS-1 was screened from sediments in the Xiamen Bay, and its related characteristics and application in environmental pollution control were analyzed. According to the morphological, biochemical properties of the strain and the results of 16S rRNA gene sequencing, the species and genera of the strain were determined. The electricity production performance of the strain was studied by constructing microbial fuel cell. At the same time, the reduction ability of hexavalent chromium, a typical heavy metal pollutant, was also explored. The bacterium was identify as Shewanella. When the initial inoculation concentration in MFC was OD₆₀₀=0.3, the maximum power density was 18 mW/m² and the current density was 243 mA/m². The bacterium could reduce Cr(Ⅵ) at pH of 7~8, and NaCl concentration of 0~6%. And this bacterium was proved to be capable of reducing Cr(Ⅵ) under the condition of wide pH and high salinity, and had good electricity production capacity. The bacterium XMS-1 had good potential in practical industrial wastewater treatment and provided scientific basis for seawater reclamation.
- microbial fuel cell /
- Cr(Ⅵ) /
- wastewater treatment /
- screening /
- bacterium

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参考文献(30)

戚姣琴,朱亮,徐向阳,等.微生物燃料电池及其在废水/废弃物处理中的应用[J].环境工程,2015, 33(3):1-5

,59.

陈月美,刘维平.沉积型微生物燃料电池处理含铜废水及产电性能[J].环境工程,2015,33(增刊1):950-954,957.

MOHAN S V, CHANDRASEKHAR K. Solid phase microbial fuel cell (SMFC) for harnessing bioelectricity from composite food waste fermentation:influence of electrode assembly and buffering capacity[J]. Bioresource Technology, 2011, 102(14):7077-7085.

LOVLEY D R. Bug juice:harvesting electricity with microorganisms[J]. Nature Reviews Microbiology, 2006, 4(7):497-508.

ZHANG B, LI W, GUO Y, et al. Microalgal-bacterial consortia:from interspecies interactions to biotechnological applications[J]. Renewable and Sustainable Energy Reviews, 2020, 118:109563.

LOGAN B E. Peer reviewed:extracting hydrogen and electricity from renewable resources[J]. Environmental Science & Technology, 2004, 38(9):160-167.

NEALSON K H, COX B L. Microbial metalion reduction and mars:extraterrestrial expectations?[J]. Current Opinion in Microbiology, 2002, 5(3):296-300.

MIN D, CHENG L, ZHANG F, et al. Enhancing extracellular electron transfer of Shewanella oneidensis MR-1 through coupling improved flavin synthesis and metal-reducing conduit for pollutant degradation[J]. Environmental Science & Technology, 2017, 51(9):5082-5089.

李明,梁湘,骆健美,等.一株产电菌嗜根考克氏菌(Kocuria rhizophila)的分离及其产电性能优化[J].环境科学学报,2015, 35(10):3078-3087.

LUO J M, YANG J, HE H H, et al. A new electrochemically active bacterium phylogenetically related to Tolumonas osonensis and power performance in MFCs[J]. Bioresource Technology, 2013, 139:141-148.

刘盛萍,吴晶,吴克,等.一株产电菌Nitratireductor sp.WJ5-4的筛选及产电分析[J].微生物学通报,2015, 42(4):665-673.

DONG G W, WANG Y P, GONG L B, et al. Formation of soluble Cr(Ⅲ) end-products and nanoparticles during Cr(Ⅵ) reduction by Bacillus cereus strain XMCr-6[J]. Biochemical Engineering Journal, 2013, 70:166-172.

VITI C, MARCHI E, DECOROSI F, et al. Molecular mechanisms of Cr(Ⅵ) resistance in bacteria and fungi[J]. FEMS Microbiology Reviews, 2014, 38(4):633-659.

王彪,黄杰勋,章晓波,等.一株海洋产电菌Shewanella sp. S2的筛选和产电分析[J].微生物学通报,2010, 37(3):342-348.

叶遥立. 微生物燃料电池生物阳极构造和产电性能研究[D]. 杭州:浙江大学, 2014.

HAUSLADEN D M, FENDORF S. Hexavalent chromium generation within naturally structured soils and sediments[J]. Environmental Science & Technology, 2017, 51(4):2058-2067.

LIN T, DING W Q, SUN L M, et al. Engineered Shewanella oneidensis reduced graphene oxide biohybrid with enhanced biosynthesis and transport of flavins enabled a highest bioelectricity output in microbial fuel cells[J]. Nano Energy, 2018, 50:639-648.

黄雪娜. 异化金属还原细菌厌氧转化对硝基苯胂酸和Cr(Ⅵ)机制的研究[D]. 合肥:中国科学技术大学, 2018.

LIU Y N, ZHANG F, LI J, et al. Exclusive extracellular bioreduction of methyl orange by azo reductase-free Geobacter sulfurreducens[J]. Environmental Science & Technology, 2017, 51(15):8616-8623.

LOVLEY D R, ELIZABETH J P P. Novel mode of microbial energy metabolism:organic carbon oxidation coupled to dissimilatory reduction of iron or manganese[J]. Applied and Environmental Microbiology, 1988, 54(6):1472-1480.

孙晓莹,陈意超,曹沁,等.耐盐菌Pseudomonas brassicacearum YZX4的筛选、鉴定及其植物促生特性[J].应用与环境生物学报,2019,25(5):1133-1138.

VENKATESWARAN K, MOSER D, DOLLHOPF M, et al. Polyphasic taxonomy of the genus Shewanella and description of Shewanella oneidensis sp[J]. International Journal of Systematic Bacteriology, 1999. 49(Part 2):705-724.

CAI P J, XIAO X, HE Y R, et al. Involvement of c-type cytochrome CymA in the electron transfer of anaerobic nitrobenzene reduction by Shewanella oneidensis MR-1[J]. Biochemical Engineering Journal, 2012, 68:227-230.

CAI P J, XIAO X, HE Y R, et al. Anaerobic biodecolorization mechanism of methyl orange by Shewanella oneidensis MR-1[J]. Applied Microbiology and Biotechnology, 2012, 93(4):1769-1776.

BENCHEIKH-LATMANI R, OBRAZTSOVA A, MACKEY M R, et al. Toxicity of Cr(Ⅲ) to Shewanella sp. strain MR-4 during Cr(Ⅵ) reduction[J]. Environmental Science & Technology, 2007, 41(1):214-220.

MIDDLETON S S, LATMANI R B, MACKEY M R, et al. Cometabolism of Cr(Ⅵ) by Shewanella oneidensis MR-1 produces cell-associated reduced chromium and inhibits growth[J]. Biotechnology and Bioengineering, 2003, 83(6):627-637.

杜艳影,刘小红,李劲,等.Shewanella oneidensis MR-1对Cr(Ⅵ)的还原及其影响因素[J].中国环境科学, 2018,38(7):2740-2745.

TANG Y J, LAIDLAW D, GANI K, et al. Evaluation of the effects of various culture conditions on Cr(Ⅵ) reduction by Shewanella oneidensis MR-1 in a novel high-throughput mini-bioreactor[J]. Biotechnology and Bioengineering, 2006, 95(1):176-184.

HUANG J X, SUN B L, ZHANG X B. Electricity generation at high ionic strength in microbial fuel cell by a newly isolated Shewanella marisflavi EP1[J]. Applied Microbiology and Biotechnology, 2010, 85(4):1141-1149.

王彪, 陈新华,徐方成, 等. 碳源和盐度对深海产电菌Shewanella sp. DS1产电能力影响[C]//2010年电力与能源工程学术会议. 武汉大学, 2010:259-262.

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