游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响

吴代顺; 常焕焕; 陈翠忠; 杨浩; 侯红勋; 孙洪伟

doi:10.13205/j.hjgc.202103012

游离氨(FA)对SBR短程硝化过程微生物菌群结构及多样性的影响

doi: 10.13205/j.hjgc.202103012

1. 福建技术师范学院海洋与生化工程学院近海流域环境测控治理福建省高校重点实验室, 福建福清 350300;
2. 兰州交通大学环境与市政工程学院, 兰州 730070;
3. 安徽国祯环保节能科技股份公司, 合肥 230031;
4. 烟台大学环境与材料工程学院, 山东烟台 264005

详细信息

作者简介:
吴代顺(1976-),男,副教授,主要研究方向为水污染控制工程。765102712@qq.com

通讯作者:
孙洪伟(1976-),男,教授,主要研究方向为水体污染控制。12821306@qq.com

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- 被引次数: 0
出版历程
- 收稿日期: 2020-10-15
- 网络出版日期: 2021-07-19

EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS

1. College of Marine and Biochemical Engineering, Fujian Provincial Key Laboratory of Coastal Basin Environmental Measurement and Control, Fujian Normal University, Fuqing 350300, China;
2. College of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
3. Anhui Guozhen Environmental Protection and Energy Saving Technology Co., Ltd, Hefei 230031, China;
4. College of Environmental and Material Engineering, Yantai University, Yantai 264005, China

摘要

摘要: 为探究游离氨（FA）对硝化过程影响的机理，试验以人工模拟废水为研究对象，基于16S rRNA基因-Illumina MiSeq高通量测序技术，采用4组平行的SBR反应器（进水FA浓度分别控制为0.5，5，10，15 mg/L，分别记为R_0.5、R₅、R₁₀和R₁₅），探究了微生物在不同FA浓度条件下的群落组成和结构特征。结果表明：FA会显著影响系统内微生物菌群结构和功能。R_0.5的α多样性指数（包括Chao1、ACE、Shannon和Simpson指数）在4组反应器中均为最大，说明R_0.5的物种多样性最高，而R₁₅的物种多样性最低。此外，在微生物门水平上，变形菌门Proteobacteria（45.9%~70.5%）和拟杆菌门Bacteroidetes（11.8%~41.3%）最具优势，且变形菌门（Proteobacteria）的相对丰度随着FA浓度升高而升高。在微生物属水平上，动胶菌属Zoogloea和陶厄氏菌属Thauera最具优势，且亚硝化单胞菌属（Nitrosomonas）和硝化螺旋菌属（Nitrospira）在R₁₀中丰度明显高于其他3个系统。基于LEfSe分析，共获得了25个具有显著差异的微生物标记物，从而得到了各FA浓度条件下在微生物学分类水平上的菌群关键生物标记物。
- 游离氨(FA) /
- 亚硝酸盐氧化细菌 /
- 高通量测序技术 /
- 群落组成 /
- LEfSe分析
Abstract: Based on 16S rRNA genes-Illumina MiSeq high-throughput sequencing, this study was aimed to investigate the community composition and structural characteristics of microorganisms under different free ammonia (FA) concentrations (0.5, 5, 10, 15 mg/L), and it was settled in four parallel laboratory-scale sequencing batch reactors (SBRs, denoted as R_0.5, R₅, R₁₀ and R₁₅) to better understand how FA concentrations influenced the nitrification. The results showed that FA could significantly affect the composition, population structure and function of the microbial community in the system. The diversity index(including Chao1, ACE, Shannon and Simpson index) of R_0.5 was the largest among the four groups of reactors, indicating that species diversity of R_0.5 was the highest, while the lowest was achieved in R₁₅. In addition, Proteobacteria (45.9%~70.5%) and Bacteroidetes (11.8%~41.3%) were found to be major groups with higher relative abundance at the phylum level, and the relative abundance of Proteobacteria increased with the increase of FA concentration. At the microbial level, Zoogloea and Thauera were the dominant generas, and Nitrosomonas and Nitrospira had significantly higher abundance in R₁₀ than the other systems. A total of 25 groups of microorganisms with significant differences were obtained based on LEfSe analysis, thereby key biomarkers of the microflora were obtained at the microbiological classification level, under each FA concentration.
- free ammonia (FA) /
- nitrite oxidizing bacteria /
- high-throughput sequencing technology /
- community composition /
- LEfSe analysis

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参考文献(20)

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