中文核心期刊
CSCD来源期刊(核心库)
中国科技核心期刊
RCCSE中国核心学术期刊
JST China 收录期刊

留言板

尊敬的读者、作者、审稿人, 关于本刊的投稿、审稿、编辑和出版的任何问题, 您可以本页添加留言。我们将尽快给您答复。谢谢您的支持!

姓名
邮箱
手机号码
标题
留言内容
验证码

接种不同菌源的餐厨垃圾发酵代谢途径及产己酸效能分析

郭志超 徐先宝 徐婷婷 赵爱华 邰俊 刘亚男 薛罡 李响

郭志超, 徐先宝, 徐婷婷, 赵爱华, 邰俊, 刘亚男, 薛罡, 李响. 接种不同菌源的餐厨垃圾发酵代谢途径及产己酸效能分析[J]. 环境工程, 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023
引用本文: 郭志超, 徐先宝, 徐婷婷, 赵爱华, 邰俊, 刘亚男, 薛罡, 李响. 接种不同菌源的餐厨垃圾发酵代谢途径及产己酸效能分析[J]. 环境工程, 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023
GUO Zhi-chao, XU Xian-bao, XU Ting-ting, ZHAO Ai-hua, TAI Jun, LIU Ya-nan, XUE Gang, LI Xiang. ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023
Citation: GUO Zhi-chao, XU Xian-bao, XU Ting-ting, ZHAO Ai-hua, TAI Jun, LIU Ya-nan, XUE Gang, LI Xiang. ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023

接种不同菌源的餐厨垃圾发酵代谢途径及产己酸效能分析

doi: 10.13205/j.hjgc.202109023
基金项目: 

国家自然科学基金面上项目(518781337);上海市国资委技术创新和能级提升项目(2018001)。

详细信息
    作者简介:

    郭志超(1996-),男,硕士研究生,主要研究方向为固废处理与资源化。1404598602@qq.com

    通讯作者:

    李响(1987-),男,博士,副教授,主要研究方向为固废处理与资源化。lix@dhu.edu.cn

ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM

  • 摘要: 采用活性污泥、颗粒污泥、酒曲以及酒糟接种餐厨垃圾进行发酵产有机酸,比较了4种菌源在批次发酵条件下的溶出、水解和酸化效率。在此基础上,探究了不同电子供体对碳链增长产己酸的影响。结果表明:利用活性污泥接种餐厨垃圾发酵可产生高光学活性L-乳酸(L-乳酸浓度为22.2 g/L,光学活性为82.3%);颗粒污泥接种餐厨垃圾发酵可产生浓度为4.2 g COD/L的己酸,具有较强产己酸能力。以酒曲和酒糟作为接种菌源时,丁酸浓度分别为21.6 g COD/L(酒曲)和20.4 g COD/L(酒糟),丁酸是碳链增长的重要电子受体,利用酒曲和酒糟接种餐厨垃圾发酵具有很强的产己酸潜力;外加电子供体后,酒糟组己酸浓度达到8.8 g COD/L(以乳酸为电子供体),酒曲组己酸浓度可达到10.4 g COD/L(以乙醇为电子供体)。微生物群落分析发现,优势菌群链球菌属(Streptococcus)分别占酒曲组和酒糟组总菌属丰度的80%以及55%,与高浓度己酸的产生有着较强相关性。
  • [1] ZHOU M M,YAN B H,WONG J W C,et al.Enhanced volatile fatty acids production from anaerobic fermentation of food waste:a mini-review focusing on acidogenic metabolic pathways[J].Bioresource Technology,2018,248:68-78.
    [2] 夏旻,毕珠洁,张瑞娜,等.上海市餐厨垃圾理化特性及资源化预处理对策研究[J].环境卫生工程,2013,21(6):1-2.
    [3] YAN B H,SELVAM A,WONG J W C.Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors[J].Bioresource Technology,2014,168:64-71.
    [4] SHEN F,YUAN H R,PANG Y Z,et al.Performances of anaerobic co-digestion of fruit & vegetable waste (FVW) and food waste (FW):single-phase vs.two-phase[J].Bioresource Technology,2013,144:80-85.
    [5] ABREU A A,TAVARES F,ALVES M M,et al.Garden and food waste co-fermentation for biohydrogen and biomethane production in a two-step hyperthermophilic-mesophilic process[J].Bioresource Technology,2019,278:180-186.
    [6] OLESKOWICZ-POPIEL P,KADAR Z,HEISKE S,et al.Co-production of ethanol,biogas,protein fodder and natural fertilizer in organic farming:evaluation of a concept for a farm-scale biorefinery[J].Bioresource Technology,2012,104:440-446.
    [7] FEI Q,FU R Z,SHANG L A,et al.Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment[J].Bioprocess and Biosystems Engineering,2015,38(4):691-700.
    [8] ZHENG J,GAO M,WANG Q H,et al.Enhancement of L-lactic acid production via synergism in open co-fermentation of Sophora flavescens residues and food waste[J].Bioresource Technology,2017,225:159-164.
    [9] WU Q L,BAO X,GUO W Q,et al.Medium chain carboxylic acids production from waste biomass:current advances and perspectives[J].Biotechnology Advances,2019,37(5):599-615.
    [10] ZHAO J W,ZHANG C,WANG D B,et al.Revealing the underlying mechanisms of how sodium chloride affects short-chain fatty acid production from the co-fermentation of waste activated sludge and food waste[J].Acs Sustainable Chemistry & Engineering,2016,4(9):4675-4684.
    [11] COOK S M,SKERLOS S J,RASKIN L,et al.A stability assessment tool for anaerobic co-digestion[J].Water Research,2017,112:19-28.
    [12] CHEN Y G,LUO J Y,YAN Y Y,et al.Enhanced production of short-chain fatty acid by co-fermentation of waste activated sludge and kitchen waste under alkaline conditions and its application to microbial fuel cells[J].Applied Energy,2013,102(S1):1197-1204.
    [13] WU S L,SUN J,CHEN X M,et al.Unveiling the mechanisms of medium-chain fatty acid production from waste activated sludge alkaline fermentation liquor through physiological,thermodynamic and metagenomic investigations[J].Water Research,2020,169:1-12.
    [14] ZHU X Y,TAO Y,LIANG C,et al.The synthesis of n-caproate from lactate:a new efficient process for medium-chain carboxylates production[J].Scientific Reports,2015,5:14360.
    [15] CARVAJAL-ARROYO J M,CANDRY P,ANDERSEN S J,et al.Granular fermentation enables high rate caproic acid production from solid-free thin stillage[J].Green Chemistry,2019,21(6):1330-1339.
    [16] YU J N,HUANG Z X,WU P,et al.Performance and microbial characterization of two-stage caproate fermentation from fruit and vegetable waste via anaerobic microbial consortia[J].Bioresource Technology,2019,284:398-405.
    [17] GILDEMYN S,MOLITOR B,USACK J G,et al.Upgrading syngas fermentation effluent using Clostridium kluyveri in a continuous fermentation[J].Biotechnology for Biofuels,2017,10:1-15.
    [18] TAO Y,ZHU X Y,WANG H,et al.Complete genome sequence of Ruminococcaceae bacterium CPB6:a newly isolated culture for efficient n-caproic acid production from lactate[J].Journal of Biotechnology,2017,259:91-94.
    [19] LOWRY O H,ROSEBROUGH N J,FARR A L,et al.Protein measurement with the Folin phenol reagent[J].The Journal of biological chemistry,1951,193(1):265-275.
    [20] GOEL R,MINO T,SATOH H,et al.Enzyme activities under anaerobic and aerobic conditions inactivated sludge sequencing batch reactor[J].Water Research,1998,32(7):2081-2088.
    [21] MU H,CHEN Y G.Long-term effect of ZnO nanoparticles on waste activated sludge anaerobic digestion[J].Water Research,2011,45(17):5612-5620.
    [22] XU X B,ZHANG W J,GU X,et al.Stabilizing lactate production through repeated batch fermentation of food waste and waste activated sludge[J].Bioresource Technology,2020,300:122709.
    [23] NYBROE O,JORGENSEN P E,HENZE M.Enzyme-activities in waste-water and activated-sludge[J].Water Research,1992,26(5):579-584.
    [24] ZHANG D P,SPADARO D,VALENTE S,et al.Cloning,characterization,expression and antifungal activity of an alkaline serine protease of Aureobasidium pullulans PL5 involved in the biological control of postharvest pathogens[J].International Journal of Food Microbiology,2012,153(3):453-464.
    [25] LIANG S,GLINIEWICZ K,MENDES-SOARES H,et al.Comparative analysis of microbial community of novel lactic acid fermentation inoculated with different undefined mixed cultures[J].Bioresource Technology,2015,179:268-274.
    [26] REDCORN R,ENGELBERTH A S.Identifying conditions to optimize lactic acid production from food waste co-digested with primary sludge[J].Biochemical Engineering Journal,2016,105:205-213.
    [27] ANGENENT L T,RICHTER H,BUCKEL W,et al.Chain elongation with reactor microbiomes:open-culture biotechnology to produce biochemicals[J].Environmental Science & Technology,2016,50(6):2796-2810.
    [28] ZHANG W J,LI X,ZHANG T,et al.High-rate lactic acid production from food waste and waste activated sludge via interactive control of pH adjustment and fermentation temperature[J].Chemical Engineering Journal,2017,328:197-206.
    [29] TASHIRO Y,KANEKO W,SUN Y Q,et al.Continuous D-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp lactis QU 41[J].Applied Microbiology And Biotechnology,2011,89(6):1741-1750.
    [30] BI D X,CHU D Q,ZHU P,et al.Utilization of dry distiller's grain and solubles as nutrient supplement in the simultaneous saccharification and ethanol fermentation at high solids loading of corn stover[J].Biotechnology Letters,2011,33(2):273-276.
    [31] COMA M,VILCHEZ-VARGAS R,ROUME H,et al.Product diversity linked to substrate usage in chain elongation by mixed-culture fermentation[J].Environmental Science & Technology,2016,50(12):6467-6476.
    [32] KUCEK L A,NGUYEN M,ANGENENT L T.Conversion of L-lactate into n-caproate by a continuously fed reactor microbiome[J].Water Research,2016,93:163-171.
    [33] TARASOV A L,BORZENKOV I A,BELYAYEV S S.Investigation of the trophic relations between anaerobic microorganisms from an underground gas repository during methanol utilization[J].Microbiology,2011,80(2):180-187.
    [34] CHEN Y,JIANG X,XIAO K,et al.Enhanced volatile fatty acids (VFAs) production in a thermophilic fermenter with stepwise pH increase:investigation on dissolved organic matter transformation and microbial community shift[J].Water Research,2017,112:261-268.
    [35] AGLER M T,SPIRITO C M,USACK J G,et al.Chain elongation with reactor microbiomes:upgrading dilute ethanol to medium-chain carboxylates[J].Energy & Environmental Science,2012,5(8):8189-8192.
    [36] KIM B C,SEUNG JEON B,KIM S,et al.Caproiciproducens galactitolivorans gen.nov.,sp.nov.,a bacterium capable of producing caproic acid from galactitol,isolated from a wastewater treatment plant[J].International Journal of Systematic and Evolutionary Microbiology,2015,65(12):4902-4908.
    [37] AGLER M T,WERNER J J,ITEN L B,et al.Shaping reactor microbiomes to produce the fuel precursorn-butyrate from pretreated cellulosic hydrolysates[J].Environmental Science & Technology,2012,46(18):10229-10238.
    [38] KHOR W C,ANDERSEN S,VERVAEREN H,et al.Electricity-assisted production of caproic acid from grass[J].Biotechnol Biofuels,2017,10(1):180-191.
  • 加载中
计量
  • 文章访问数:  12
  • HTML全文浏览量:  0
  • PDF下载量:  2
  • 被引次数: 0
出版历程
  • 收稿日期:  2020-10-26
  • 网络出版日期:  2022-01-21

目录

    /

    返回文章
    返回