硫自养反硝化耦合厌氧氨氧化脱氮系统快速启动及微生物群落分析

赵记楠; 刘思韵; 单瑛琦; 刘畅; 田梦园; 李柏林

doi:10.13205/j.hjgc.202406002

硫自养反硝化耦合厌氧氨氧化脱氮系统快速启动及微生物群落分析

doi: 10.13205/j.hjgc.202406002

赵记楠^1,2,
刘思韵^1,2,
单瑛琦^1,2,
刘畅^1,2,
田梦园^1,2,
李柏林^1,2, ,

1. 武汉理工大学资源与环境工程学院, 武汉 430070;
2. 矿物资源加工与环境湖北省重点实验室, 武汉 430070

基金项目:

国家大学生创新创业训练项目(S202210497322)

详细信息

作者简介:
赵记楠(2002-),男,本科,主要研究方向为污水生物自养脱氮技术。zhao12136@whut.edu.cn

通讯作者:
李柏林(1983-),男,教授,主要研究方向为水体污染治理及修复。bolly1221@whut.edu.cn

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- 被引次数: 0
出版历程
- 收稿日期: 2022-11-15
- 网络出版日期: 2024-07-11

RAPID START-UP AND MICROBIAL COMMUNITY ANALYSIS OF A SULFUR AUTOTROPHIC DENITRIFICATION COUPLED ANAEROBIC AMMONIA OXIDATION DENITRIFICATION SYSTEM

ZHAO Jinan^1,2,
LIU Siyun^1,2,
SHAN Yingqi^1,2,
LIU Chang^1,2,
TIAN Mengyuan^1,2,
LI Bolin^{1,2
, ,}

1. School of Resources and Environmental Engineering, Wuhan University of Technology, Wuhan 430070, China;
2. Hubei Key Laboratory of Mineral Resources Processing and Environment, Wuhan 430070, China

摘要

摘要: 以硫代硫酸钠为电子供体,采用梯度增加基质负荷的方式,在SBR反应器中完成硫自养反硝化(SAD)工艺启动。通过接种厌氧氨氧化(Anammox)污泥,采用pH和NO^-₃-N/NH⁺₄-N联合调控策略进行研究,实现硫自养反硝化耦合厌氧氨氧化(SAD/A)自养脱氮系统快速启动及稳定运行。结果表明:30 d成功将反硝化污泥驯化为SAD污泥,90 d后,SBR反应器成功富集SAD污泥,系统NO^-₃-N去除率可达到85%以上。SAD/A系统2种种泥最佳接种比为3∶1,运行14 d后SAD/A系统成功启动。30 d后,系统NO^-₃-N去除率可达到85%,NH⁺₄-N去除率达到80%。pH值为8,且ρ(NO^-₃-N)/ρ(NH⁺₄-N)为1.8时,耦合系统脱氮效率最佳,实现稳定运行。高通量测序结果表明:SAD/A系统整体的物种多样性和丰富度增加,优势微生物菌属为厌氧氨氧化菌属(Candidatus Brocadia)和硫杆菌属(Thiobacillus),二者相对丰度相近,系统耦合度较好。
- 厌氧氨氧化 /
- 硫自养反硝化 /
- SAD/A /
- NO^-₂-N积累 /
- 菌群结构
Abstract: In this paper, the sulfur autotrophic denitrification (SAD) process start-up was accomplished in an SBR reactor using sodium thiosulfate as an electron donor, and a gradual increase in substrate loading. The rapid start-up and stable operation of the sulfur autotrophic denitrification coupled with anaerobic ammonia oxidation (SAD/A) autotrophic denitrification system was developed by inoculating Anammox sludge with a combined pH and NO^-₃-N/NH⁺₄-N regulation strategy. The results showed that in 30 days, the denitrification sludge had been domesticated into SAD sludge. After 90 days, the SBR reactor had been successfully enriched with SAD sludge, and more than 85% of NO^-₃-N had been removed from the system. The SAD/A system was successfully started after 14 days of operation with an optimal inoculation ratio of 3∶1 for both two species of sludge. After 30 days, the NO^-₃-N removal rate of the system could reach 85% and the NH⁺₄-N removal rate could reach 80%. The coupled system’s best denitrification efficiency was achieved at a pH of 8 and a NO^-₃-N/NH⁺₄-N ratio of 1.8, and it operated steadily. High-throughput sequencing results showed that the overall species diversity and richness in the SAD/A system was increased after coupling, and the dominant microbial genera were Candidatus Brocadia and Thiobacillus, with similar relative abundances, indicating a good coupling effect of SAD/A.
- Anammox /
- sulfur autotrophic denitrification /
- SAD/A /
- NO^-₂-N accumulation /
- microbial community structure

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