不同HRT挂膜策略对生物膜启动阶段生物量及种群结构的影响

江乐勇; 赵颖出; 孙事昊; 邢亿园; 王博; 彭永臻; 张璐晶; 庞洪涛; 杨慎华

doi:10.13205/j.hjgc.202509009

不同HRT挂膜策略对生物膜启动阶段生物量及种群结构的影响

doi: 10.13205/j.hjgc.202509009

1. 信开环境投资有限公司, 北京 101101;
2. 北京工业大学城镇污水处理与资源化利用技术国家工程实验室, 北京 100024;
3. 中国社会科学院数量经济与技术经济研究所, 北京 100732

基金项目:

北京市通州区科技创新人才资助项目（CXTD2023004）；国家自然科学基金面上项目（22176007）

详细信息

作者简介:
江乐勇（1981—），男，博士，高级工程师，主要研究方向为城市水污染控制与水环境治理。jiangleyong@sdic.com.cn

通讯作者:
孙事昊（1991—），男，博士，高级工程师，主要研究方向为城市污水脱氮除磷与生物除臭。ssh_fire@163.com

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出版历程
- 收稿日期: 2024-11-26
- 网络出版日期: 2025-11-05
- 刊出日期: 2025-09-01

Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure

1. Xinkai Environment Investment Co., Ltd., Beijing 101101, China;
2. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100024, China;
3. Institute of Quantitative & Technological Economics, CASS, Beijing 100732, China

摘要

摘要: 挂膜是生物膜工艺运行的关键步骤，水力停留时间（HRT）是其关键影响因素之一。建立4个纯生物膜反应器，以北方某污水处理厂原水作为进水，探究不同HRT对生物膜量、生物膜种群结构的影响。试验结果显示：短HRT策略（2 h）在挂膜前15 d生物膜量可达到（21.23±1.2） mg SS/carrier，相比不进水、长HRT策略（6 h）和长短HRT切换（6 h→2 h）3种策略，在挂膜前期具有显著的优势。扫描电镜（SEM）和高通量测序（Illumina Miseq）分析15，32，57 d的样品的结果显示，4个反应器生物膜群落结构均随挂膜时间的延长发生了显著变化，变多的菌属主要有Acinetobacter属、Pseudomonas属，变少的菌属主要有Ottowia属、Acidovorax属等。但是，不同HRT策略之间群落结构的α多样性H检验、β多样性ANOSIM/Adonis检验结果均显示出4组之间无显著性差异。这说明不同HRT策略会影响生物膜量，但对生物膜群落结构的影响不显著，基于此结论提出了“先挂膜再驯化”的功能性生物膜启动方法。
- 生物膜 /
- 挂膜 /
- 纯生物膜反应器 /
- MBBR
Abstract: Formation process is a key step for a moving bed biofilm reactor （MBBR）， and the hydraulic retention time （HRT） is one of the key influencing factors. This study established four biofilm reactors， using raw water from a sewage treatment plant as the influent to explore the effects of different HRT strategies on biomass and microbial community structure of biofilm. Results revealed that the short HRT strategy （2 h） achieved a biomass of （21.23±1.2） mg SS/carrier in 15 d， which showed significant advantages compared with the three strategies： no water intake， long HRT strategy （6 h） and long-short HRT switching strategy （6 h → 2 h）. SEM and MiSeq Illumina analysis of the samples at 15 d， 32 d， and 57 d revealed that the microbial community structure of the biofilm changed significantly over time. Acinetobacter and Pseudomonas were the main genera with an increasing trend， while Ottowia and Acidovorax were the main genera with a decreasing trend. However， the H-test for α-diversity and the ANOSIM/Adonis test for β-diversity of the microbial community structure revealed no significant differences among the four groups under different HRT strategies. This indicates that different HRT strategies influence the biomass of the biofilm but have no significant effect on its microbial community structure. Based on these findings， this study proposes a functional biofilm initiation method described as "biofilm attachment first， domestication afterward".
- biofilm /
- biofilm attachment /
- pure biofilm reactor /
- MBBR

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