菖蒲基生物炭填料强化人工湿地去除抗生素

许晓毅; 杨天宝; 吴玮; 黄天寅; 崔双双; 徐乐悠; 吴兵党; 杨晶晶; 杜淑娟; 庄金龙

doi:10.13205/j.hjgc.202511004

菖蒲基生物炭填料强化人工湿地去除抗生素

doi: 10.13205/j.hjgc.202511004

许晓毅^1,2,
杨天宝^1,2,
吴玮¹,
黄天寅^1,2,
崔双双³,
徐乐悠¹,
吴兵党^1,2,
杨晶晶^1,2,
杜淑娟³,
庄金龙^1,2, ,

1. 苏州科技大学环境科学与工程学院, 江苏苏州 215009;
2. 苏州市海绵城市技术重点实验室, 江苏苏州 215009;
3. 苏州高新水质净化有限公司, 江苏苏州 215009

基金项目:

苏州市科技项目（2022SS18）；苏州市水利科技项目（2022001）

详细信息

作者简介:
许晓毅（1974—），女，博士，教授，主要从事水污染控制技术及生物修复研究。xuxiaoyiskd@usts.edu.cn

通讯作者:
庄金龙（1994—），男，博士，主要从事废水生物处理理论与技术研究。zhuangjl0906@163.com

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出版历程
- 收稿日期: 2024-05-15
- 录用日期: 2024-07-02
- 修回日期: 2024-06-20
- 网络出版日期: 2026-01-09

Acorus calamus-based biochar filler enhances removal of antibiotics in constructed wetlands

1. School of Environmental Science and Engineering, Suzhou University of Science and Technology, Suzhou 215009, China;
2. Key Laboratory of Suzhou Sponge City Technology, Suzhou 215009, China;
3. Suzhou New District Water Purification Co., Ltd., Suzhou 215009, China

摘要

摘要: 针对城市污水处理厂尾水抗生素残留问题，利用菖蒲基生物炭填料强化人工湿地（construct wetlands， CWs）去除磺胺甲恶唑（sulfamethoxazole， SMX）及红霉素（Erythromycin， ERY），并优化了其运行方式和运行参数，解析抗生素抗性基因（antibiotic resistance genes， ARGs）的分布及微生物结构。结果表明：1）水平潜流人工湿地对SMX、ERY和氮素营养盐的去除率均显著高于垂直潜流人工湿地（P<0.05），具备较优水质深度净化性能。2）当菖蒲基生物炭填料投加量为4%，水力停留时间为3 d时，CWs对SMX和ERY的去除效果最佳，分别可达90.5%和92.7%。3）生物炭填料投加量由2%提高至4%和6%，CWs出水中ARGs分别下降了81.2%和74.4%，基质中耐药细菌（Rhodococcus和Luteolibacter）丰度下降16%，降低水生态风险。研究结果可为CWs系统长期有效去除污水处理厂尾水抗生素残留提供重要参考。
- 人工湿地 /
- 生物炭填料 /
- 抗生素 /
- 抗生素抗性基因 /
- 投加量
Abstract: To solve the critical issue of antibiotic residues in the effluent of urban wastewater treatment plants， this study investigated the enhancement of constructed wetlands （CWs） through the application of calamus-based biochar fillers to improve the removal efficiency of sulfamethoxazole （SMX） and erythromycin （ERY）. The research focuses on optimizing the operational methods and parameters of CWs， and analyzing the distribution of antibiotic resistance genes （ARGs） and the microbial community structure. The findings were as follows： 1） horizontal subsurface flow constructed wetland demonstrated a markedly higher removal rate for SMX， ERY， and nitrogenous nutrients compared to vertical subsurface flow constructed wetland （P<0.05）， indicating its superior performance in deep water purification； 2）the optimal removal efficiencies for SMX and ERY， reaching 90.5% and 92.7%， respectively， were achieved when the dosage of calamus-based biochar fillers was set at 4% and the hydraulic retention time was maintained at 3 days； 3） the substantial reduction of antibiotic resistance genes in the constructed wetlands effluent was 81.2% when biochar filler dosage increased from 2% to 4%， but the substantial reduction of antibiotic resistance genes in the constructed wetlands effluent was 74.4% when biochar filler dosage increased from 2% to 4%. Meanwhile， the absolute abundance of sul1 decreased from 1.2×10⁹ copies/g in 2% biochar filler to 9.6×10⁸ copies/g in 4% biochar filler， and further to 3.8×10⁸ copies/g in 6% biochar filler， respectively. Additionally， the abundance of antibiotic-resistant bacteria， specifically Rhodococcus and Luteolibacter， within the substrate significantly decreased by 16%. These would reduce the ecological safety risks in water bodies. These findings provide new insights for enhancing constructed wetland systems， enabling the sustainable and efficient removal of antibiotic residues from municipal wastewater treatment plant effluents.
- construct wetland /
- biochar filler /
- antibiotics /
- antibiotic resistance genes /
- dosage

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