联氨对厌氧氨氧化生物膜脱氮性能的短期影响

娄雨晴; 吴祎雪; 陈淞; 苑泉; 张艳萍; 孙迎雪

doi:10.13205/j.hjgc.202512009

联氨对厌氧氨氧化生物膜脱氮性能的短期影响

doi: 10.13205/j.hjgc.202512009

北京工商大学生态环境学院, 北京 100048

基金项目:

国家重点研发计划项目 “污水资源化与新污染物风险控制技术”（2022YFE0104900）

详细信息

作者简介:
娄雨晴（1998—），女，硕士研究生，主要研究方向为废水处理与资源化利用。louyuqing07@hotmail.com

通讯作者:
孙迎雪（1973—），女，教授，主要研究方向为工业废水处理技术与资源化利用。sunyx@th.btbu.edu.cn

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出版历程
- 收稿日期: 2023-05-15
- 录用日期: 2023-08-21
- 修回日期: 2023-07-14
- 网络出版日期: 2026-01-09

Short-term effect of hydrazine on operation and nitrogen removal performance of anammox biofilm

School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China

摘要

摘要: 针对厌氧氨氧化（Anammox）在城市污水应用中稳定性较差的难题，通过添加联氨（N₂H₄）提高厌氧氨氧化菌的活性，从基质降解速率、N₂H₄降解速率、化学反应计量比、胞外聚合物（EPS）、血红素和微生物群落等指标变化进行分析，研究不同浓度N₂H₄在短期内对厌氧氨氧化生物膜脱氮性能的影响。结果表明：加入N₂H₄后增加了脱氮系统内NO^-₂-N的消耗率，减少了NO^-₃-N生成率。当N₂H₄浓度为40 mg/L时，系统强化脱氮效果最佳，TN去除速率（NRR）为78.35 mg/（L·d），较未投加N₂H₄时提高了3倍。从生物质中提取的EPS含量分析表明，N₂H₄浓度为20 mg/L时，TB-EPS含量最高为10.55 mg/g，比对照组增加了0.9倍，其含量的增长显著提高了生物膜的稳定性。在加入20 mg/L N₂H₄的实验组中测得血红素含量为3.00 μmol/g，较对照组提高了53%。因此，在短期内城市废水中添加20~40 mg/L N₂H₄可促进厌氧氨氧化菌的生长以及厌氧氨氧化生物膜系统的稳定。
- 厌氧氨氧化 /
- 联氨 /
- 生物膜 /
- 胞外聚合物 /
- 血红素
Abstract: With the addition of hydrazine （N₂H₄）， Anammox’s low activity in municipal wastewater applications can be increased. To investigate the short-term effects of various N₂H₄ concentrations on the nitrogen removal capability of ANAMMOX biofilms， the change in matrix degradation rate， N₂H₄ degradation rate， the stoichiometric ratio of chemical reaction， extracellular polymer （EPS）， heme， and microbial community were analyzed. The results showed that the addition of N₂H₄ increased the consumption rate of NO^-₂-N and decreased the formation rate of NO^-₃-N. When the concentration of N₂H₄ was 20 mg/L， the highest total nitrogen removal efficiency （NRR）of 78.35 mg/（L·d） was achieved， which was three times higher than the control group. The analysis of EPS extracted from biomass showed that， the maximum concentration of TB-EPS was 10.55 mg/g when the concentration of N₂H₄was 20 mg/L， that was 0.9 times higher than the control， and the increase in TB-EPS concetration can inprove the stability of the biofilm； the highest concentration of heme was 3.00 μmol/g when the concentration of N₂H₄ was 20 mg/L. As a result， short-term addition of 20 to 40 mg/L of N₂H₄ can promote the growth of AnAOB and enchance the stability of the ANAMMOX biofilm system.
- Anammox /
- N₂H₄ /
- biofilm /
- EPS /
- heme

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