短程反硝化-厌氧氨氧化耦合MBR强化脱氮中试

庄彦华; 周俊宏; 夏阳光; 陶昱明; 杨小丽

doi:10.13205/j.hjgc.202512012

短程反硝化-厌氧氨氧化耦合MBR强化脱氮中试

doi: 10.13205/j.hjgc.202512012

1. 南京市给排水工程设计院有限公司, 南京 210036;
2. 东南大学土木工程学院, 南京 211189

基金项目:

江苏省重点研发计划（BE2021619）；南京水务集团有限公司技术攻关课题（H202310299）

详细信息

作者简介:
庄彦华（1983—），男，高级工程师，主要从事污水处理技术研究与设计。13913954712@163.com

通讯作者:
庄彦华（1983—），男，高级工程师，主要从事污水处理技术研究与设计。13913954712@163.com

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出版历程
- 收稿日期: 2024-11-18
- 录用日期: 2025-01-08
- 修回日期: 2024-12-21
- 网络出版日期: 2026-01-09

A pilot study of partial denitrification-anammox coupled with MBR process to enhance nitrogen removal

1. Nanjing Water Supply and Drainage Engineering Design Institute Co., Ltd., Nanjing 210036, China;
2. School of Civil Engineering, Southeast University, Nanjing 211189, China

摘要

摘要: 针对传统生物脱氮碳源需求大、能耗高等问题，构建了短程反硝化-厌氧氨氧化-膜生物反应器耦合工艺（SPDA-MBR）中试装置，进水由实际城镇污水配制，探究SPDA-MBR工艺对城镇污水的强化脱氮效果并解析其机理。结果表明：短程反硝化反应器（PD池）在17.8~27.9 ℃，厌氧氨氧化膜生物反应器（Anammox-MBR）在33.2~36.3 ℃的运行条件下，SPDA-MBR工艺平均TN去除率为89.59%；其中PD池和Anammox-MBR的脱氮负荷分别为0.13 kg/（m³·d）和0.15 kg/（m³·d）（以N计），对应脱氮贡献分别为53.94%和46.06%。高通量测序结果显示：PD池中成功富集Thauera（索氏菌属）和Thermomonas（热单胞菌属）两种短程反硝化菌属，相对丰度分别为1.7%和4.01%；组合运行前后Anammox-MBR中典型AnAOB菌属Candidatus_Jettenia受冲击影响，其相对丰度由13.87%降低至6.16%，但厌氧氨氧化伴生菌属SM1A02和SWB02相对丰度上升，说明在城镇污水处理过程中，复杂菌种间的协同作用有利于维持较高的厌氧氨氧化效果；研究进一步表明，SPDA-MBR工艺强化城镇污水稳定高效脱氮的关键是维持厌氧氨氧化与反硝化等多种脱氮途径的协同。
- SPDA-MBR /
- 短程反硝化 /
- 厌氧氨氧化 /
- 城镇污水处理
Abstract: Aiming at the problems of large carbon demand and high energy consumption of the traditional wastewater treatment process， this study constructed a pilot plant of SPDA-MBR. The influent was prepared using real urban wastewater to investigate the denitrification effect of the SPDA-MBR process on urban wastewater and analyze its mechanism. The results indicated that， under the operational conditions of 17.8 to 27.9 ℃ for the partial denitrification reactor （PD tank）， and 33.2 to 36.3 ℃ for the anaerobic ammonium oxidation membrane bioreactor （Anammox-MBR）， the average TN removal rate of the SPDA-MBR process was 89.59%. The nitrogen removal loading of the PD pool and Anammox-MBR pool were 0.13 kg N/（m³·d） and 0.15 kg N/（m³·d）， respectively， contributing 53.94% and 46.06% to the total nitrogen removal. High-throughput sequencing results showed that two partial denitrifying bacteria， Thauera and Thermomonas， were successfully enriched in the PD pool， with relative abundances of 1.7% and 4.01%， respectively. The relative abundance of Candidatus_Jettenia， a typical AnAOB genus， decreased from 13.87% to 6.16%， due to the impact of disturbances before and after the operational period， but the relative abundance of anammox companion genera SM1A02 and SWB02 increased， indicating that the synergistic effect among the complex bacterial species is beneficial to maintaining a higher anammox effect in the urban wastewater treatment process. This further suggests that the key to strengthening the stable and efficient nitrogen removal of urban wastewater by the SPDA-MBR process， is to maintain the synergistic effect of anammox and denitrification and other nitrogen removal pathways.
- SPDA-MBR /
- partial denitrification /
- anammox /
- urban wastewater treatment

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