鼠李糖脂对铜绿微囊藻生长及藻毒素产生和释放的影响

邵晨曦; 倪利晓; 徐楚; 桑文璐; 张伊; 杜存浩; 李时银

doi:10.13205/j.hjgc.202601007

鼠李糖脂对铜绿微囊藻生长及藻毒素产生和释放的影响

doi: 10.13205/j.hjgc.202601007

邵晨曦^1,2,
倪利晓^1,2, ,,
徐楚^1,2,
桑文璐^1,2,
张伊^1,2,
杜存浩^1,2,
李时银³

1. 河海大学环境学院, 南京 210098;
2. 河海大学浅水湖泊综合治理与资源开发教育部重点实验室, 南京 210098;
3. 南京师范大学环境学院, 南京 210023

基金项目:

国家重点研发计划（2023YFC3208900， 2023YFC3208904）；国家自然科学基金项目（52570195）；江苏省水利科技项目（2022063）

详细信息

作者简介:
邵晨曦（2000—），女，硕士，主要研究方向为水污染控制及水生态修复。shaochenxi0623@126.com

通讯作者:
倪利晓（1973—），女，教授，主要研究方向为水污染控制及水生态修复。nilixiao@hhu.edu.cn

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- 文章访问数: 2
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出版历程
- 收稿日期: 2024-07-10
- 网络出版日期: 2026-02-26
- 刊出日期: 2026-01-22

Effects of rhamnolipids on growth， microcystin production and release of Microcystis aeruginosa

SHAO Chenxi^1,2,
NI Lixiao^{1,2
, ,},
XU Chu^1,2,
SANG Wenlu^1,2,
ZHANG Yi^1,2,
DU Cunhao^1,2,
LI Shiyin³

1. College of Environment, Hohai University, Nanjing 210098, China;
2. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China;
3. College of Environment, Nanjing Normal University, Nanjing 210023, China

摘要

摘要: 常见生物表面活性剂鼠李糖脂对部分海洋微藻具有抑制作用。为探究鼠李糖脂对铜绿微囊藻生长及产毒能力的影响以及作用机制，测定了鼠李糖脂胁迫下铜绿微囊藻生长、藻毒素产生及释放、胞内氧自由基、丙二醛含量以及细胞膜形态的变化。结果表明：8 mg/L鼠李糖脂对处于对数生长期初期的铜绿微囊藻，在10 d时的抑制率已超过90%，试验结束时抑藻率达到95.5%，在21 d内藻细胞生长得到显著控制。鼠李糖脂胁迫增强了单个藻细胞的产毒能力，mcyD基因上调至空白组的1.1倍，但显著降低了藻毒素产生总量，且释放到胞外的藻毒素含量并未发生明显差异。鼠李糖脂胁迫下，藻细胞内超氧阴离子含量过载，造成细胞膜脂质过氧化并分解成丙二醛，膜结构被破坏，胞内藻毒素释放到外界。该研究结果可为鼠李糖脂的实际应用提供理论基础。
- 鼠李糖脂 /
- 铜绿微囊藻 /
- 微囊藻毒素 /
- 生物表面活性剂 /
- 超氧阴离子
Abstract: Rhamnolipid， a common biosurfactant， has an inhibitory effect on some marine microalgae. To investigate the effects of rhamnolipids on the growth and microcystin production of Microcystis aeruginosa and the impact mechanism， the growth， microcystin production and release， intracellular oxygen radicals， malondialdehyde content， and cell membrane morphology of Microcystis aeruginosa under rhamnolipid stress were measured. The results indicated that 8 mg/L rhamnolipid inhibited more than 90% of Microcystis aeruginosa at the beginning of the logarithmic growth period by day 10， and 95.5% by the end of the test， with significant inhibition of algal growth within 21 days. Rhamnolipids enhanced the MC-LR production capacity of the algal cells， with the mcyD gene up-regulated to 1.1 times that of the blank group， but the total amount of MC-LR produced by the algal cells was significantly reduced， and no significant difference in the amount of extracellular MC-LR released was observed. The overloading of superoxide anion in algal cells under rhamnolipid stress caused lipid peroxidation of cell membranes， decomposition of membrane lipids into malondialdehyde， disruption of membrane structure， and consequent release of intracellular MC-LR. The result can provide a theoretical basis for the practical application of rhamnolipids.
- rhamnolipid /
- Microcystis aeruginosa /
- microcystin /
- biosurfactant /
- superoxide anion

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