以硫铁矿和乙酸盐为基质的自养异养还原去除水中高氯酸盐

李晴晴; 王怡冰; 毛洋莉; 万东锦; 何巧冲

doi:10.13205/j.hjgc.202601008

以硫铁矿和乙酸盐为基质的自养异养还原去除水中高氯酸盐

doi: 10.13205/j.hjgc.202601008

1. 河南工业大学环境工程学院, 郑州 450001;
2. 郑州市水安全与水生态技术重点实验室, 郑州 450001

基金项目:

国家自然科学基金青年基金（42207066）；郑州市科技局自然科学项目-协同创新专项（22ZZRDZX13）；河南工业大学青年骨干教师培育计划（21421214）

详细信息

作者简介:
李晴晴（2001—），女，硕士研究生，主要研究方向为水处理。ll11231026@163.com

通讯作者:
何巧冲（1989—），女，博士，副教授，主要研究方向为水处理。qiaochonghe@haut.edu.cn

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出版历程
- 收稿日期: 2025-01-05
- 网络出版日期: 2026-02-26
- 刊出日期: 2026-01-22

Perchlorate removal from water by pyrite-autotrophic and acetate-heterotrophic reduction process

1. College of Environmental Engineering, Henan University of Technology, Zhengzhou 450001, China;
2. Zhengzhou Key Laboratory of Water Safety and Water Ecology Technology, Zhengzhou 450001, China

摘要

摘要: 采用硫铁矿自养协同乙酸钠异养微生物还原法去除水中高氯酸盐，构建填充床反应器，并探究了水中高氯酸盐去除性能。实验结果表明，硫铁矿和乙酸钠可共同为微生物还原高氯酸盐提供电子供体，将污水中的高氯酸盐［Cl（Ⅶ）］还原为氯离子（Cl^-）。反应器运行前15 d，高氯酸盐去除率可达85%以上；随着温度的降低，去除率有所下降（约60%）；随着温度升高以及水力停留时间增加，去除性能提高，最终高氯酸盐去除率稳定在95%以上。反应运行前期（0~35 d），硫铁矿作为微生物电子供体还原高氯酸盐占主导；反应器运行35 d后，由于硫铁矿的钝化，乙酸钠异养对高氯酸盐还原起主导作用。动力学分析表明，高氯酸盐降解符合零级动力学模型，高氯酸盐去除速率为0.48~0.70 mg/（L·h）。利用X射线光电子能谱（XPS）技术对硫铁矿进行表征，结果表明硫铁矿中S^2-和Fe（Ⅱ）被氧化为SO₄^2-和Fe（Ⅲ）。高通量测序表明，以硫铁矿和乙酸钠为基质的微生物还原体系中优势菌属为Romboutsia，Ferruginibacter 和Dokdonella。研究为高氯酸盐污染废水修复提供了一种潜在方法。
- 微生物还原 /
- 硫铁矿 /
- 乙酸钠 /
- 高氯酸盐 /
- 优势菌属
Abstract: In this paper， a combination of pyrite autotrophic and sodium acetate-based heterotrophic reduction process was used for removing perchlorate from water， and the removal performance was investigated in a pyrite-packed reactor. Results showed that perchlorate ［Cl（Ⅶ）］ was effectively reduced into chloride ions （Cl^-） using pyrite and acetate as electron donors. In the early stage of reactor operation （from day 0 to day 15）， the perchlorate removal efficiency was over 85%； as the temperature decreased， the removal efficiency decreased and ultimately stabilized at 60% above. As the temperature increased and the hydraulic retention time increased， the removal performance improved， and the final removal efficiency of perchlorate stabilized at over 95%. Pyrite-based autotrophic reduction was dominant （from 0 to day 35） first， while acetate-based heterotrophic reduction was dominant in the later stage for perchlorate removal. Kinetic analysis indicated that the degradation process of perchlorate followed a zero-order kinetic model， and the perchlorate removal rate was 0.48 mg/（L·h） to 0.70 mg/（L·h）. The characterization of pyrite by X-ray photoelectron spectroscopy （XPS） technology showed that S^2-and Fe（Ⅱ） in the pyrite were oxidized into SO₄^2- and Fe（Ⅲ）， respectively. The dominant bacterial genera for the removal of perchlorate in the reactor were Romboutsia， Ferruginibacter， and Dokdonellai. This study provides a potential approach for the remediation of perchlorate-contaminated wastewater.
- biological reduction /
- pyrite /
- sodium acetate /
- perchlorate /
- dominant genus

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