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微生物电解池系统阴极处理2-氯-4-硝基苯酚废水的性能与机理探究

刘靖,  杨筱玲,  罗萃,  周中敏,  梁一夫,  郭勤,  黄冬根

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文章导航 > 环境工程  > 2026 >  44(2): 40-49
刘靖, 杨筱玲, 罗萃, 周中敏, 梁一夫, 郭勤, 黄冬根. 微生物电解池系统阴极处理2-氯-4-硝基苯酚废水的性能与机理探究[J]. 环境工程, 2026, 44(2): 40-49. doi: 10.13205/j.hjgc.202602005
引用本文: 刘靖, 杨筱玲, 罗萃, 周中敏, 梁一夫, 郭勤, 黄冬根. 微生物电解池系统阴极处理2-氯-4-硝基苯酚废水的性能与机理探究[J]. 环境工程, 2026, 44(2): 40-49. doi: 10.13205/j.hjgc.202602005
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LIU Jing, YANG Xiaoling, LUO Cui, ZHOU Zhongmin, LIANG Yifu, GUO Qin, HUANG Donggen. Performance and mechanism of cathodic treatment of 2-chloro-4-nitrophenol contaminated wastewater in microbial electrolysis cells[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(2): 40-49. doi: 10.13205/j.hjgc.202602005
Citation: LIU Jing, YANG Xiaoling, LUO Cui, ZHOU Zhongmin, LIANG Yifu, GUO Qin, HUANG Donggen. Performance and mechanism of cathodic treatment of 2-chloro-4-nitrophenol contaminated wastewater in microbial electrolysis cells[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(2): 40-49. doi: 10.13205/j.hjgc.202602005
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微生物电解池系统阴极处理2-氯-4-硝基苯酚废水的性能与机理探究

doi: 10.13205/j.hjgc.202602005

  • 1. 南昌大学 资源与环境学院, 南昌 330031;

  • 2. 南昌师范学院 外国语学院, 南昌 330032;

  • 3. 江西省交通设计研究院有限责任公司, 南昌 330052

基金项目: 

国家自然科学基金项目“鼠李糖脂作用下氯硝柳胺在泥微生物电解系统迁移与共基质降解特性”（41967046）；江西省交通设计研究院科技项目“基于短程硝化-MBR工艺的高速公路服务区中水回用系统应用”（院科技字［2023］9号）

详细信息
    作者简介:

    刘靖（2001—），男，硕士研究生，主要研究方向为水污染处理研究。1582367207@qq.com

    通讯作者:

    黄冬根（1964—），男，教授，主要研究方向为有机污染物降解机制与水污染控制技术研究、友好环境材料基础应用研究等。dghuang1017@163.com

Performance and mechanism of cathodic treatment of 2-chloro-4-nitrophenol contaminated wastewater in microbial electrolysis cells

  • 1. School of Resources and Environment, Nanchang University, Nanchang 330031, China;

  • 2. School of Foreign Languages, Nanchang Normal University, Nanchang 330032, China;

  • 3. Jiangxi Provincial Transportation Design and Research Institute Co., Ltd., Nanchang 330052, China

    摘要
  • 摘要: 2-氯-4-硝基苯酚（2C4NP）是一种典型的氯代硝基酚（CNPs）类污染物，具有持久性和较高的环境毒性。目前，关于微生物电解池系统（MECs）降解2C4NP的研究较少，其降解机理尚不明确。研究构建双室MECs，系统考察了外电压、2C4NP初始浓度及共基质种类对MECs阴极降解2C4NP的影响。通过扫描电镜（SEM）分析微生物形貌，并利用高效液相色谱（HPLC）、离子色谱（IC）和LC/MS/MS质谱分析技术对2C4NP及其降解中间体进行了定性和定量分析，提出了MECs阴极降解2C4NP的机制。结果表明：施加适当的外电压可显著促进2C4NP的降解，其初始浓度对降解速率影响显著，且不同共基质条件下降解过程符合不同的反应动力学规律。在最佳条件（外加电压0.5 V，共基质为葡萄糖，2C4NP初始浓度30 mg/L）下，2C4NP的72 h去除率和脱氯率分别达到96.77%和67.74%。SEM分析结果显示，阴极表面微生物呈现团聚态，增强了微生物之间的电子传递效率，为2C4NP高效降解提供了基础。HPLC、IC和LC/MS/MS分析表明，2C4NP在降解过程中产生了4-硝基苯酚、对乙酰氨基酚、2-氯酚等中间产物，部分脱除的氯和氮分别以Cl-和NO3-形式存在，这表明2C4NP在MECs阴极室的降解主要通过还原脱氯与脱硝基反应实现。研究初步揭示了双室MECs阴极降解2C4NP的机理，为氯代硝基酚类污染物的降解提供了理论依据和技术支持。
    Abstract: 2-Chloro-4-nitrophenol （2C4NP） is a typical chloronitrophenol （CNPs）， characterized by persistence and high environmental toxicity. Currently， research on the degradation of 2C4NP by microbial electrolysis cells （MECs） is still limited， and the degradation mechanism remains unclear. In this study， a two-chamber MECs was constructed to systematically investigate the effects of external voltage， initial concentration of 2C4NP， and co-substrate types on the cathodic degradation of 2C4NP. Scanning electron microscopy （SEM） was used to analyze the microbial morphology， and high-performance liquid chromatography （HPLC）， ion chromatography （IC）， and LC/MS/MS were employed for qualitative and quantitative analysis of 2C4NP and its degradation intermediates， thereby elucidating the mechanism of 2C4NP degradation at the MECs cathode. Results showed that the application of an appropriate external voltage significantly promoted the degradation of 2C4NP. The initial concentration of 2C4NP had a significant impact on the degradation rate， and the degradation process followed different reaction kinetics under different co-substrate conditions. Under the optimal conditions （external voltage of 0.5 V， co-substrate of glucose， and initial concentration of 2C4NP at 30 mg/L）， the 72 h removal rate and dechlorination rate of 2C4NP reached 96.77% and 67.74%， respectively. SEM analysis revealed that microorganisms on the cathode surface aggregated， enhancing electron transfer efficiency among microorganisms and providing a basis for efficient degradation. HPLC， IC， and LC/MS/MS analyses indicated that the degradation of 2C4NP produced intermediate products such as 4-nitrophenol， paracetamol， and 2-chlorophenol. The partially removed chlorine and nitrogen were detected as Cl- and NO3-. These results suggest that the degradation of 2C4NP in the MEC cathode chamber mainly occurs through reductive dechlorination and denitration reactions.
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  • 收稿日期:  2025-02-26
  • 网络出版日期:  2026-04-11
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