李氏禾超富集铬和镍的根际过程研究

胡子健; 刘冰冰; 刘子昕; 蒋萍萍

doi:10.13205/j.hjgc.202602022

李氏禾超富集铬和镍的根际过程研究

doi: 10.13205/j.hjgc.202602022

桂林理工大学地球科学学院, 广西桂林 541006

基金项目:

国家自然科学基金项目“磷素强化李氏禾修复铬污染土壤的机制研究”（52300198）；广西自然科学基金面上项目“超富集植物青葙共富集镉锰的关键根际过程研究”（2025GXNSFAA069249）

详细信息

作者简介:
胡子健（2005—），女，本科生。3142682529@qq.com

通讯作者:
蒋萍萍（1990—），女，博士，副教授，主要研究方向为有害元素的地球化学研究、受损环境的生态修复。jiangpp@glut.edu.cn

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出版历程
- 收稿日期: 2025-01-27
- 网络出版日期: 2026-04-11
- 刊出日期: 2026-02-01

Rhizosphere process of hyperaccumulation of chromium and nickel by Leersia hexandra Swartz

College of Earth Science, Guilin University of Technology, Guilin 541006, China

摘要

摘要: 李氏禾是国内首次发现并报道的铬超富集植物，也是镍和铜富集植物。选取李氏禾为研究对象，利用平板光极和原位酶谱技术，研究了重金属铬、镍胁迫下李氏禾根际微域pH值的时空变化规律，以及重金属胁迫对李氏禾根际土壤酶活性的影响。研究结果表明：1）在重金属铬、镍胁迫下，李氏禾根际pH值显著降低。CK组根际周围土壤pH值在6.57~7.13内波动；在复合胁迫下的pH值下降程度较为剧烈，pH值在6.00~6.57内波动。根际酸化可活化重金属，促进超富集植物对重金属的吸收，这可能是李氏禾超富集铬和富集镍的重要机制。2）李氏禾根际区域为酶活性的热点区域。在重金属铬、镍胁迫下，β-葡萄糖苷酶活性因重金属胁迫而增加，酸性磷酸酶活性因重金属的胁迫而减少。CK组的酶活性总体在400~600 pmol/（cm²·h）内波动，而在重金属胁迫下，根际土壤酶活性下降至200~400 pmol/（cm²·h）区间，说明在重金属铬、镍胁迫下，李氏禾根际区域对酶活性产生显著影响，这可能与李氏禾在胁迫下根际所发生的生理机制相关。
- 李氏禾 /
- 铬 /
- 镍 /
- 平板光级 /
- 原位酶谱 /
- 根际过程
Abstract: Phytoremediation technology is considered to be one of the most promising remediation methods for complex contaminated soil. Leersia hexandra Swartz is a chromium hyperaccumulator first discovered and reported in China， and also a nickel and copper hyperaccumulator plant. Previous studies have been carried out on the physiological and molecular characteristics of chromium， nickel， and copper accumulation in L. hexandra. However， the rhizosphere process and the mechanism of symbiotic regulation of co-hyperaccumulation of chromium and nickel by L. hexandra remain unclear. Therefore， in this paper， L. hexandra was selected as the research object， and the spatiotemporal variation of pH value in rhizosphere microdomain under the heavy metal stress of chromium and nickel， and the influence on enzyme activity in the rhizosphere soil of L. hexandra were studied by using the planar optode technique and in-situ enzyme spectrometry. The results showed that： 1） under the stress of heavy metals chromium and nickel， the rhizosphere pH value of L. hexandra will be significantly reduced. The pH value of the soil around the rhizosphere in the CK group fluctuated between 6.57 and 7.13， but decreased sharply under the compound stress， and the pH value fluctuated between 6.00 and 6.57. Rhizosphere acidification is an important mechanism for the activation and absorption of heavy metals by hyperaccumulator plants， which may also be an important mechanism for the hyperaccumulation of chromium and nickel in L. hexandra. 2） The rhizosphere zone of L. hexandra was the hotspot of enzyme activity. Under the stress of chromium and nickel， the activity of β-glucosidase increased， and the activity of acid phosphatase decreased. The enzyme activity in the CK group fluctuated between 400 pmol/（cm²·h） and 600 pmol/（cm²·h）， while the enzyme activity in rhizosphere soil decreased to 200 to 400 pmol/（cm²·h） under heavy metal stress. These results indicated that the rhizosphere region of L. hexandra had a significant effect on enzyme activity under the stress of chromium and nickel， which may be related to the physiological mechanism of the rhizosphere of L. hexandra under stress.
- Leersia hexandra Swartz /
- chromium /
- nickel /
- planar optode /
- in-situ enzyme spectrum /
- rhizosphere process

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