长短链群体感应信号分子协同调控对电活性生物膜代谢磺胺甲恶唑效能的影响

龙禧; 张宝雯; 田婧; 熊健冰; 孙文域; 汪涛

doi:10.13205/j.hjgc.202604006

长短链群体感应信号分子协同调控对电活性生物膜代谢磺胺甲恶唑效能的影响

doi: 10.13205/j.hjgc.202604006

五邑大学环境与化学工程学院, 广东江门 529020

基金项目:

广东省自然科学基金面上项目“胞外呼吸菌驱动头孢制药废水厌氧消化机制与过程调控” （2024A1515010681）；国家自然科学基金项目（52200067）

详细信息

作者简介:
龙禧(2000—),女,研究生,主要研究方向为厌氧消化处理废水。longxi66880000@163.com

通讯作者:
汪涛(1989—),男,副教授,主要研究方向为废水、固废生物处理与资源化。pkucnedu@yeah.net

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出版历程
- 收稿日期: 2025-12-04
- 网络出版日期: 2026-06-06
- 刊出日期: 2026-04-01

Effects of cooperative regulation by long-and short-chain quorum sensing signaling molecules on metabolic efficacy of sulfamethoxazole in electroactive biofilms

School of Environmental and Chemical Engineering, Wuyi University, Jiangmen 529020, China

摘要

摘要: 高浓度磺胺甲恶唑（SMX）废水对厌氧微生物具有显著抑制作用，导致厌氧消化过程有机物降解效率降低及甲烷产量下降。研究通过添加短链（C6-HSL）和长链（C12-HSL）信号分子，研究其在微生物电解池耦合厌氧消化（MEC-AD）体系中对厌氧电活性生物膜系统的构建、性能和抗生素抗性基因（ARGs）的影响。结果表明，与无添加信号分子的对照组相比，添加了C6-HSL的T1组、C12-HSL的T2组和同时添加了二者的T3组的SMX去除率相对值分别提升了9.26%、7.44%和10.67%，甲烷产率分别提升了20.4%、16.9%和23.1%。信号分子N-酰基高丝氨酸内酯（AHL）通过促进胞外聚合物的生成，显著改善了电活性微生物在阳极电极上的附着。微生物群落分析表明，AHL提高了微生物群落多样性，并调节了关键功能菌的丰度。在属水平上，Georgenia对不同类型信号分子表现出显著的差异性响应，T1和T3组的相对丰度上升16.77%和36.47%，T2组则下降了15.99%。ARGs检测结果显示，单一信号分子提高了intI1、sul1和sul2的基因丰度；而T3组（复合AHLs）则表现出更温和的响应，其sul2丰度相较于对照组甚至降低了4.92%，这表明复合AHLs可能通过协同作用，在一定程度上抑制了ARGs潜在宿主的增殖。研究首次揭示了短链与长链复合AHLs在促进电活性生物膜构建，维持微生物群落稳定，调节ARGs传播风险的作用。研究结果为发展基于群体感应调控的抗生素废水处理与风险管理提供可行路径。
- 群体感应 /
- 磺胺甲恶唑 /
- 厌氧消化 /
- 电活性生物膜 /
- 抗性基因
Abstract: Wastewater containing high concentrations of sulfamethoxazole （SMX） significantly inhibits anaerobic microorganisms， leading to reduced organic matter degradation efficiency and decreased methane yield in anaerobic digestion processes. This study investigated the effects of adding short-chain （C6-HSL） and long-chain （C12-HSL） signaling molecules on the construction， performance， and antibiotic resistance genes （ARGs） of anaerobic electroactive biofilm systems within a microbial electrochemically coupled anaerobic digestion （MEC-AD） system. Results indicated that， compared to the control group （without added signaling molecules）， the relative SMX removal rates in the C6-HSL group （T1）， the C12-HSL group （T2）， and the dual-treated group （T3） increased by 9.26%， 7.44%， and 10.67%， respectively， while methane production rates increased by 20.4%， 16.9%， and 23.1%， respectively. The signaling molecule N-acyl-γ-lactones （AHLs） significantly enhanced the attachment of electroactive microorganisms to the anode electrode by promoting extracellular polymer production. Microbial community analysis revealed that AHLs enhanced microbial diversity and regulated the abundance of key functional bacteria. At the genus level， Georgenia exhibited significantly differential responses to different signal molecules： relative abundance increased by 16.77% and 36.47% in T1 and T3 groups， respectively， while decreasing by 15.99% in T2 group. ARGs analysis results showed that single signaling molecules increased the abundance of intl1， sul1， and sul2. However， the T3 group exhibited a milder response， with sul2 abundance decreasing by 4.92% compared to the control group. This finding indicates that the synergistic effect of composite AHLs helps mitigate the amplification of potential ARG-carrying bacteria. This study reveals， for the first time， the role of short-chain and long-chain composite AHLs in promoting electroactive biofilm formation， maintaining microbial community stability， and regulating ARG transmission risks. It can provide a feasible pathway for developing antibiotic wastewater treatment and risk management strategies based on quorum sensing regulation.
- quorum sensing /
- sulfamethoxazole /
- anaerobic digestion /
- electroactive biofilm /
- antibiotic resistance genes

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