低浓度全氟辛烷磺酸对活性污泥厌氧发酵的影响

冷思贤; 程尚; 赵红亮; 韦艺媛; 钱庆渝; 潘伟亮

doi:10.13205/j.hjgc.202607010

低浓度全氟辛烷磺酸对活性污泥厌氧发酵的影响

doi: 10.13205/j.hjgc.202607010

1. 重庆交通大学环境水利工程重庆市工程实验室, 重庆 400074;
2. 重庆市畜牧技术推广总站, 重庆 401120;
3. 重庆丞果环境科技有限公司, 重庆 400037

基金项目:

重庆市技术创新与应用发展专项重点项目（CSTB2022TIAD-KPX0201）；重庆市自然科学基金项目（CSTB2022NSCQ-MSX1438）；重庆市研究生联合培养基地（JDLHPYJD2024004）

详细信息

作者简介:
冷思贤（2000—），女，硕士研究生，主要研究方向为污泥资源化利用。lengsixian@163.com

通讯作者:
程尚（1981—），男，副研究员，主要研究方向为畜禽粪污资源化利用技术。chengshang3@126.com

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出版历程
- 收稿日期: 2025-09-09
- 网络出版日期: 2026-09-01

Effects of perfluorooctane sulfonate （ PFOS ） on anaerobic fermentation of activated sludge

1. Engineering Laboratory of Environmental Hydraulic Engineering of Chongqing Municipal Development and Reform Commission, Chongqing Jiaotong University, Chongqing 400074, China;
2. Chongqing Animal Husbandry Technology Extension Station, Chongqing 401120, China;
3. Chongqing Chengguo Environmental Technology Co., Ltd., Chongqing 400037, China

摘要

摘要: 低浓度全氟辛烷磺酸（PFOS）对活性污泥厌氧发酵过程的作用机制仍不明确。设置0.5，1.0，4.5 μg/g TSS 3个PFOS浓度水平，系统评估了PFOS对污泥厌氧发酵过程中有机质溶解释放和氮磷转化的影响。实验监测了可溶性化学需氧量（SCOD）、可溶性蛋白、多糖、NH⁺₄-N、PO^3-₄-P及三维荧光特征，结合动态变化规律揭示低浓度PFOS的效应特征。结果表明：PFOS显著促进了SCOD、蛋白和多糖的释放，其中在45 °C，14 d和PFOS浓度4.5 μg/g TSS条件下SCOD峰值较对照组提高约28%，表明可生物降解有机质积累增加。NNH⁺₄-N浓度整体升高并伴随波动，而O^3-₄-P释放受到抑制，提示氮磷转化途径可能受干扰。三维荧光分析进一步显示，低浓度PFOS增强了芳香族蛋白和微生物副产物信号，验证了基质溶解释放效应。综合结果揭示了低浓度PFOS在厌氧发酵过程中表现出“前期促进、后期潜在抑制”的双重作用特征。该研究可为理解PFOS在污泥厌氧消化中的环境行为提供数据支撑，并为污泥资源化利用与新污染物风险评估提供参考。
- 活性污泥 /
- 厌氧发酵 /
- PFOS /
- 低浓度效应 /
- 三维荧光
Abstract: The effects of low concentrations of perfluorooctane sulfonate （PFOS） on the anaerobic fermentation of activated sludge remain unclear. In this study， three PFOS concentration levels （0.5， 1.0， and 4.5 μg/g TSS） were applied to systematically evaluate their influence on organic matter solubilization and nitrogen and phosphorus transformations during anaerobic fermentation. Soluble chemical oxygen demand （SCOD）， soluble protein， polysaccharides， NH⁺₄-N， PO^3-₄-P， and three-dimensional excitation-emission matrix （3D-EEM） fluorescence spectra were monitored to characterize the dynamic responses under PFOS exposure. The results showed that PFOS significantly enhanced the release of SCOD， protein， and polysaccharides. At 45 °C after 14 days with a PFOS concentration of 4.5 μg/g TSS， the SCOD peak was approximately 28% higher than that of the control， indicating an increased accumulation of biodegradable organic matter. NH⁺₄-N concentrations increased overall with notable fluctuations， whereas PO^3-₄-P release was inhibited， suggesting potential interference with nitrogen and phosphorus transformation pathways. 3D-EEM analysis further revealed strengthened signals of aromatic proteins and microbial by-products under PFOS exposure， confirming the solubilization effect on the sludge matrix. Overall， these findings demonstrate a dual effect of low-level PFOS during anaerobic fermentation， characterized by initial promotion of organic matter release followed by potential inhibition associated with nutrient transformation. These results provide new insights into the environmental behavior of PFOS in sludge anaerobic digestion and have important implications for sludge resource utilization and the risk assessment of emerging contaminants.
- activated sludge /
- anaerobic fermentation /
- PFOS /
- low concentration effect /
- 3D-EEM fluorescence spectroscopy

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