城市污泥施用对矿区矸石土成分和微生物种群的影响研究

张博; 王佳玮; 杨朝凤; 卢佳飞; 李莉; 胡筱敏

doi:10.13205/j.hjgc.202607023

城市污泥施用对矿区矸石土成分和微生物种群的影响研究

doi: 10.13205/j.hjgc.202607023

1. 汉江师范学院化学与环境工程学院生物资源与生态环境保护十堰市重点实验室, 湖北十堰 442000;
2. 东北大学资源与土木工程学院, 沈阳 110819

基金项目:

中国科协青年人才托举工程博士生专项计划；汉江师范学院校级重点研究项目“基于微生物促进C/N/P循环的城市污泥土地利用可行性研究”（XJ2025A07）；十堰市引导性科研计划项目“城市污泥源微生物驱动土壤碳循环与固碳增汇机制研究”（25Y040）

详细信息

作者简介:
张博（1995—），男，讲师，主要研究方向为城市污泥资源化、矿区生态修复。Zhangbo2505@163.com

通讯作者:
胡筱敏（1958—），男，教授，主要研究方向为水污染控制。hxmin＿jj@163.com

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出版历程
- 收稿日期: 2025-11-09
- 网络出版日期: 2026-09-01

Research on effects of municipal sludge application on composition and microbial communities of mine waste rock soil

1. Shiyan Key Laboratory of Biological Resources and Eco-environmental Protection, College of Chemical and Environmental Engineering Hanjiang Normal University, Shiyan 442000, China;
2. School of Resources and Civil Engineering, Northeastern University, Shenyang 110819, China

摘要

摘要: 随着人口的快速增长和城市社会生活的迅猛发展，城市污泥（municipal sludge，MS）综合利用成为亟需解决的一大难题。MS中含有丰富的有机物和作物生长所必需的营养物质，将其作为土壤肥料应用于矿区矸石生态修复具有良好的应用前景。然而，从土壤微生物学角度开展MS施用对土壤健康和生态安全评价的研究鲜少。为此，通过盆栽试验，探究了MS和堆肥污泥（CMS）施用对矿区矸石土生态恢复的影响，并采用高通量测序技术分析了土壤微生物群落结构与多样性变化，最后利用网络分析和相关性热图阐明微生物学驱动机制。结果表明：MS、CMS施用后，有机质含量从20.38 g/kg（三级）提升至38.52 g/kg（二级），速效氮、速效磷、速效钾分别从四级、六级和二级水平，提升至一级、四级和一级水平，黑麦草鲜重、地上高度、根长和茎粗均显著提升。Venn和Heatmap分析表明，较低施用量（<1.5 kg/m²）有助于提高微生物种群的丰富度和多样性。研究证实，MS可作为有效的矸石土改良剂，建议在实际矿区生态修复中控制施用量在1.5 kg/m²以内，并重点关注重金属与盐分的长期动态，以实现安全与可持续的土地再利用。
- 城市污泥 /
- 矿区矸石 /
- 生态修复 /
- 微生物群落 /
- 高通量测序
Abstract: The rapid population growth and accelerating urban development have made the comprehensive utilization of municipal sludge （MS） an urgent challenge. MS contains substantial organic matter and essential nutrients for crop growth， making it a promising soil amendment for the ecological restoration of mine waste rock. However， research evaluating the impact of MS application on soil health and ecological safety from a soil microbiology perspective remains understudied. Therefore， this study investigated the effects of MS and composted municipal sludge （CMS） on the ecological restoration of mine waste rock soil through pot experiments. High-throughput sequencing technology was employed to analyze changes in soil microbial community structure and diversity. Finally， network analysis and correlation heatmaps were utilized to elucidate the microbial driving mechanisms. The results indicated that after MS and CMS application， organic matter content increased from 20.38 g/kg （Level 3） to 38.52 g/kg （Level 2）. The levels of available nitrogen， phosphorus， and potassium rose from Level 4， 6， 2，to Level 1， 4， 1， respectively. Fresh weight， aboveground height， root length， and stem diameter of ryegrass all increased significantly. Venn diagram and heatmap analyses indicated that lower application rates （<1.5 kg/m²） enhanced microbial community richness and diversity. This study confirms municipal sludge as an effective amendment for mine waste rock soil. It is recommended to limit application rates below 1.5 kg/m² in practical mine ecological restoration projects， with particular attention to long-term dynamics of heavy metals and salinity to ensure safe and sustainable land reuse.
- municipal sludge /
- mine waste rock /
- ecological restoration /
- microbial communities /
- high-throughput sequencing

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