地下水铬污染微生物修复技术：研究热点、作用机理与环境因子解析

杨延梅; 贺万壮; 刘泉利; 党秋玲; 苏婧; 刘玥

doi:10.13205/j.hjgc.202608024

地下水铬污染微生物修复技术：研究热点、作用机理与环境因子解析

doi: 10.13205/j.hjgc.202608024

杨延梅¹,
贺万壮^1,2,
刘泉利²,
党秋玲²,
苏婧²,
刘玥^2, ,

1. 重庆交通大学河海学院, 重庆 400074;
2. 中国环境科学研究院, 北京 100012

基金项目:

国家重点研发计划项目“土壤与地下水多要素协同作用的环境基准制定方法”（2023YFC3708700）

详细信息

通讯作者:
刘玥（1992—），女，助理研究员，主要从事地下水污染防治等研究。liu.yue@craes.org.cn

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出版历程
- 收稿日期: 2025-07-01

Microbial remediation technology for chromium pollution in groundwater： bibliometric analysis and exploration of key environmental factors

1. Hehai College, Chongqing Jiaotong University, Chongqing 400074, China;
2. Chinese Academy of Environmental Sciences, Beijing 100012, China

摘要

摘要: 微生物修复是近年来地下水铬污染修复领域广泛使用的修复技术之一。以Web of Science核心数据库收录的文献资料为数据来源，利用CiteSpace、VOSviewer、Pajek文献计量软件，针对近15年（2010—2024年）全球地下水微生物修复领域的相关文献，从发文量、关键词分析、政策分析3个方面进行分析，系统梳理了该领域研究的发展态势和热点。结果表明：关于地下水微生物修复技术领域的研究论文共441篇，近15年来发文趋势总体呈上升趋势；该领域发展可分为3个阶段，早期研究阶段（2010—2016年）主要集中在铬污染的基本治理方法，深入探索阶段（2017—2019年）关注微生物修复过程的内在机制，扩展研究阶段（2020—2024年）重点转移到对修复过程进行优化。通过文献分析揭示生物吸附与生物还原是铬污染微生物修复的主要机理，同时总结了温度、pH、铬初始浓度及反应时间4种环境因子对微生物修复的影响机制，通过相关性分析和主成分分析，确定温度是影响修复效率的关键因素，并总结不同优势菌种的去除效率及机理，为实际应用中菌种的选择提供参考。建议未来研究聚焦于微生物群落的协同作用、纳米材料的联用以及优化环境条件方面，以提升铬污染的微生物修复效率。
- 铬污染 /
- 微生物修复 /
- 地下水 /
- 文献计量学 /
- 影响因素
Abstract: Microbial remediation is one of the widely used remediation techniques in the field of groundwater chromium pollution remediation. Using literature collected from the Web of Science core^® database as the data source， CiteSpace， VOSviewer and Pajek bibliometric software were used to analyze the relevant literature in the field of global groundwater microbial remediation in the past 15 years （2010 to 2024） from three aspects： publication volume， keyword analysis， and policy analysis. The development trend and hotspots of research in this field were systematically sorted out. The results showed that there were a total of 441 research papers in the field of groundwater microbial remediation technology， and the trend of publication has been on the rise in the past 15 years. The development of this field can be divided into three stages： The early research stage （2010 to 2016） mainly focused on the basic treatment methods of chromium pollution； the in-depth exploration stage （2017 to 2019） focused on the intrinsic mechanisms of microbial remediation processes； the expanded research stage （2020 to 2024） shifted its focus to remediation process optimization. Through literature analysis， it is revealed that biological adsorption and biological reduction are the main mechanisms of chromium contaminated microbial remediation. The impact mechanism of four environmental factors， including temperature， pH， initial chromium concentration and reaction time， on microbial remediation was determined through correlation analysis and principal component analysis. Temperature was identified as the key factor affecting remediation efficiency， and the removal efficiency and mechanism of different dominant bacterial strains were summarized， providing reference for the selection of bacterial strains in practical applications. In the future， it is recommended to focus on the synergistic effects of microbial communities， the combination of nanomaterials， and the optimization of environmental conditions to enhance the microbial remediation efficiency of chromium pollution.
- chromium pollution /
- microbial remediation /
- groundwater /
- bibliometrics /
- influencing factors

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参考文献(63)

[1]	GUO L L，KANG S G，WANG Q，et al. Permeable reactive barrier for chromium contaminated groundwater remediation：an overview[J]. Environmental Engineering（China），2020，38（6）：9-15. 郭丽莉，康绍果，王祺，等. 渗透式反应墙技术修复铬污染地下水的研究进展[J]. 环境工程，2020，38（6）：9-15.
[2]	MOLINARI S，MAGRO M，CARBONE C，et al. Environmental implications of one-century COPRs evolution in a single industrial site：from leaching impact to sustainable remediation of CrVI polluted groundwater[J]. Chemosphere，2021，283：131211.
[3]	WANG X R，LI L，YAN X H，et al. Progress in remediation of chromium-contaminated sites[J]. Environmental Engineering（China），2020，38（6）：1-8. 王兴润，李磊，颜湘华，等. 铬污染场地修复技术进展[J]. 环境工程，2020，38（6）：1-8.
[4]	HUANG J，WU G，ZENG R，et al. Chromium contributes to human bronchial epithelial cell carcinogenesis by activating Gli2 and inhibiting autophagy[J]. Toxicology Research，2017，6：324-332.
[5]	HE J，HE C，CHEN X，et al. Comparative study of remediation of Cr（Ⅵ）-contaminated soil using electrokinetics combined with bioremediation[J]. Environmental Science and Pollution Research，2018，25：17682-17689.
[6]	LIN W H，CHEN C C，OU J H，et al. Bioremediation of hexavalent-chromium contaminated groundwater：microcosm，column，and microbial diversity studies[J]. Chemosphere，2022，295：133877.
[7]	WANG Q，GUO S，ALI M，et al. Thermally enhanced bioremediation：a review of the fundamentals and applications in soil and groundwater remediation[J]. Journal of Hazardous Materials，2022，433：128749.
[8]	New York State Department of Environmental Conservation. Technical guidance for site investigation and remediation（DER-10）[EB/OL].[ 2010-05-03]. https：//dec.ny.gov/regulatory/regulations/technical-guidance-for-site-investigation-and-remediation-der-10.
[9]	An official websites of the European Union. Directive 2014/ 80/EU[EB/OL].[ 2010-05-03]. https：//eur-lex.europa.eu/legal-content/EN/TXT/？uri=CELEX%3A32014L0080.
[10]	The State Council of the People's Republic of China. Regulations on groundwater management[EB/OL].[ 2021-11-09] https：//www.gov.cn/zhengce/content/2021-11/09/content_5649924.htm. 中华人民共和国国务院. 地下水管理条例[EB/OL]. 2021-11-09. https：//www.gov.cn/zhengce/content/2021-11/09/content_5649924.htm.
[11]	National Development and Reform Commission of the People's Republic of China. Measures for the special management of central budget investment in pollution control[EB/OL].[ 2024-04-08] https：//www.ndrc.gov.cn/xxgk/zcfb/ghxwj/202404/t20240408_1365533.html. 中华人民共和国国家发展和改革委员会. 污染治理中央预算内投资专项管理办法[EB/OL].[ 2024-04-08]. https：//www.ndrc.gov.cn/xxgk/zcfb/ghxwj/202404/t20240408_1365533.html.
[12]	Ministry of Ecology and Environment of the People's Republic of China action plan for soil pollution source prevention and control[EB/OL].[ 2014-11-09] https：//www.mee.gov.cn/xxgk2018/xxgk/xxgk03/202411/t20241108_1093746.html. 中华人民共和国生态环境部. 土壤污染源头防控行动计划[EB/OL]. 2024-11-09. https：//www.mee.gov.cn/xxgk2018/xxgk/xxgk03/202411/t20241108_1093746.html.
[13]	YAASHIKAA P R，PALANIVELU J，HEMAVATHY R V. Sustainable approaches for removing toxic heavy metal from contaminated water：a comprehensive review of bioremediation and biosorption techniques[J]. Chemosphere，2024：141933.
[14]	OTHMAN A R，IMRON M F，ISMAIL N I，et al. Biosorption of hexavalent chromium in aqueous solution by Bacillus cereus[J]. Environmental Technology& Innovation，2025，37：104015.
[15]	DITTERT I M，DE LIMA BRANDÃO H，PINA F，et al. Integrated reduction/oxidation reactions and sorption processes for Cr（Ⅵ）removal from aqueous solutions using Laminaria digitata macro-algae[J]. Chemical Engineering Journal，2014，237：443-454.
[16]	JOBBY R，JHA P，YADAV A K，et al. Biosorption and biotransformation of hexavalent chromium[Cr（Ⅵ）]：a comprehensive review[J]. Chemosphere，2018，207：255-266.
[17]	ZHENG X，LIN H，DU D，et al. Remediation of heavy metals polluted soil environment：a critical review on biological approaches[J]. Ecotoxicology and Environmental Safety，2024，284：116883.
[18]	LIN W H，CHEN S C，CHIEN C C，et al. Application of enhanced bioreduction for hexavalent chromium-polluted groundwater cleanup：microcosm and microbial diversity studies[J]. Environmental Research，2020，184：109296.
[19]	XU T S，OU J，MA C C. Research progress on the mechanism of microbial reduction of Cr（Ⅵ）[J]. Environmental Engineering（China），2015，33（1）：32-36. 徐天生，欧杰，马晨晨. 微生物还原 Cr（Ⅵ）的机理研究进展[J]. 环境工程，2015，33（1）：32-36.
[20]	LIN W H，CHIEN C C，OU J H，et al. Cleanup of Cr（Ⅵ）-polluted groundwater using immobilized bacterial consortia via bioreduction mechanisms[J]. Journal of Environmental Management，2023，339：117947.
[21]	ZHA S，YU A，WANG Z，et al. Microbial strategies for effective hexavalent chromium removal：a comprehensive review[J]. Chemical Engineering Journal，2024，489：151457.
[22]	GUO S，XIAO C，ZHOU N，et al. Speciation，toxicity，microbial remediation and phytoremediation of soil chromium contamination[J]. Environmental Chemistry Letters，2021，19（2）：1413-1431.
[23]	MA L，CHEN N，FENG C，et al. Recent advances in enhanced technology of Cr（VI）bioreduction in aqueous condition：a review[J]. Chemosphere，2024，351：141176.
[24]	PANG A，ZHANG S，ZHANG X，et al. AQDS-functionalized biochar enhances the bioreduction of Cr（Ⅵ）by Shewanella putrefaciens CN32[J]. Chemosphere，2024，363：142866.
[25]	LI W，FENG Z，ZHU X，et al. Efficient removal of Cr（Ⅵ）from coal gangue by indigenous bacteria-YZ1 bacteria：adsorption mechanism and reduction characteristics of extracellular polymer[J]. Ecotoxicology and Environmental Safety，2024，272：116047.
[26]	CHAI L，DING C，LI J，et al. Multi-omics response of Pannonibacter phragmitetus BB to hexavalent chromium[J]. Environmental Pollution，2019，249：63-73.
[27]	PRIYA A K，JALIL A A，VADIVEL S，et al. Heavy metal remediation from wastewater using microalgae：recent advances and future trends[J]. Chemosphere，2022，305：135375.
[28]	FENG L，LIU B，YAO J，et al. Extracellular bioreduction is the main Cr（Ⅵ）detoxification strategy of Bacillus sp. HL1[J]. Journal of Environmental Management，2024，358：120870.
[29]	WU S C，HSIAO W C，ZHAO Y C，et al. Hexavalent chromate bioreduction by a magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1 and the effect of magnetosome synthesis[J]. Chemosphere，2023，330：138739.
[30]	KARTHIK C，BARATHI S，PUGAZHENDHI A，et al. Evaluation of Cr（Ⅵ）reduction mechanism and removal by Cellulosimicrobium funkei strain AR8，a novel haloalkaliphilic bacterium[J]. Journal of Hazardous Materials，2017，333：42-53.
[31]	MENG Y，MA X，LUAN F，et al. Sustainable enhancement of Cr（Ⅵ）bioreduction by the isolated Cr（Ⅵ）-resistant bacteria[J]. Science of the Total Environment，2022，812：152433.
[32]	BISWAS S，JAYARAM S，PHILIP I，et al. Appraisal of the potential of endophytic bacterium Bacillus amyloliquefaciens from Alternanthera philoxeroides：a triple approach to heavy metal bioremediation，diesel biodegradation，and biosurfactant production[J]. Journal of Environmental Chemical Engineering，2024，12（5）：113454.
[33]	GU T，NIU W，WU Y，et al. The characteristics of molasses-based reductive removal of Cr（Ⅵ）from groundwater by Bacillus sp[J]. Journal of Environmental Chemical Engineering，2022，10（6）：108595.
[34]	GARG S K，SINGH K，TRIPATHI M. Optimization of process variables for hexavalent chromium biosorption by psychrotrophic Pseudomonas putida SKG-1 isolate[J]. Desalination and Water Treatment，2016，57：19865-19876.
[35]	HUANG Y，ZENG Q，HU L，et al. Bioreduction performances and mechanisms of Sporosarcina saromensis W5 for Cr（Ⅵ）removal[J]. Journal of Hazardous Materials，2021，413：125411.
[36]	XU H，ZHANG H，QIN C，et al. Groundwater Cr（Ⅵ）contamination and remediation：a review from 1999 to 2022[J]. Chemosphere，2024：142395.
[37]	TANG X，HUANG Y，LI Y，et al. Study on detoxification and removal mechanisms of hexavalent chromium by microorganisms[J]. Ecotoxicology and Environmental Safety，2021，208：111699.
[38]	MA L，CHEN N，FENG C，et al. Coupling enhancement of Chromium（Ⅵ）bioreduction in groundwater by phosphorus minerals[J]. Chemosphere，2020，240：124896.
[39]	SHI C，LÜ J，PEI Z，et al. Study on the enhancement effect of EDTA and oxalic acid on phytoremediation of Cr（Ⅵ）from soil using Datura stramonium L[J]. Ecotoxicology and Environmental Safety，2024，287：117272.
[40]	WANG Y，MO T，LUO Y，et al. Cr（Ⅵ）bioremediation mechanism of a novel Microbacterium oxydans S-1 strain[J]. Journal of Environmental Chemical Engineering，2025：117200.
[41]	XU H，REN L，JIE W，et al. Transformation of iron-minerals from natural aquifer media by sulfate-reducing bacteria：behavior，mechanism，and Cr（Ⅵ）removal[J]. Science of the Total Environment，2024，955：177021.
[42]	MIN X，ZHANG K，CHEN J，et al. Bacteria-driven copper redox reaction coupled electron transfer from Cr（Ⅵ）to Cr（Ⅲ）：a new and alternate mechanism of Cr（Ⅵ）bioreduction[J]. Journal of Hazardous Materials，2024，461：132485.
[43]	TANG L，YANG J，LIU X，et al. Biodegradation of phenanthrene-Cr（Ⅵ）co-contamination by Pseudomonas aeruginosa AO-4 and characterization of enhanced degradation of phenanthrene[J]. Science of the Total Environment，2024，918：170744.
[44]	CHUG R，MATHUR S，KOTHARI S L，et al. Maximizing EPS production from Pseudomonas aeruginosa and its application in Cr and Ni sequestration[J]. Biochemistry and Biophysics Reports，2021，26：100972.
[45]	ZHANG L，XI L，HE S，et al. Riboflavin derivatives as a novel electron transfer mediator for enhancing Cr（Ⅵ）removal by Shewanella oneidensis MR-1[J]. International Biodeterioration& Biodegradation，2024，195：105892.
[46]	CHEN L，WU Y，SHEN Q，et al. Enhancement of hexavalent chromium reduction by Shewanella oneidensis MR-1 in presence of copper nanoparticles via stimulating bacterial extracellular electron transfer and environmental adaptability[J]. Bioresource Technology，2022，361：127686.
[47]	XI L，HE S，QIN Y，et al. Biosynthesis of biogenic ferrous sulfide as a potential electron shuttle for enhanced Cr（Ⅵ）removal by Shewanella oneidensis MR-1[J]. Journal of Water Process Engineering，2024，57：104725.
[48]	GE S，GE S，ZHOU M，et al. Bioremediation of hexavalent chromate using permeabilized Brevibacterium sp. and Stenotrophomonas sp. cells[J]. Journal of Environmental Management，2015，157：54-59.
[49]	WANG X，ZHAO R，WU H，et al. Enhanced bioremediation of hexavalent chromium via Stenotrophomonas acidaminiphila 4-1 assisted with agricultural wastes-derived biochar[J]. Biochemical Engineering Journal，2024，208：109355.
[50]	LI L，SHANG X，SUN X，et al. Bioremediation potential of hexavalent chromium by a novel bacterium Stenotrophomonas acidaminiphila 4-1[J]. Environmental Technology& Innovation，2021，22：101409.
[51]	RAMLI N N，OTHMAN A R，KURNIAWAN S B，et al. Metabolic pathway of Cr（Ⅵ）reduction by bacteria：a review[J]. Microbiological Research，2023，268：127288.
[52]	LI J，TANG C，ZHANG M，et al. Exploring the Cr（Ⅵ）removal mechanism of Sporosarcina saromensis M52 from a genomic perspective[J]. Ecotoxicology and Environmental Safety，2021，225：112767.
[53]	HUANG Y，ZENG Q，HU L，et al. Column study of enhanced Cr（Ⅵ）removal and removal mechanisms by Sporosarcina saromensis W5 assisted bio-permeable reactive barrier[J]. Journal of Hazardous Materials，2021，405：124115.
[54]	OLIVEIRA J M S，POULSEN J S，FORESTI E，et al. New insights into the mechanism of azo dye biodegradation by Lactococcus lactis[J]. Journal of Environmental Chemical Engineering，2024，12（5）：113670.
[55]	XI Z，LI T，LIAO L，et al. Safety evaluation，removal characteristics，mechanism exploration，and application potential of Lactococcus lactis GFWM17，a broad-spectrum bacteriostatic strain that simultaneously removes nitrogen and sulfide[J]. Journal of Environmental Chemical Engineering，2025：116587.
[56]	AKHZARI F，NASERI T，MOUSAVI S M，et al. A sustainable solution for alleviating hexavalent chromium from water streams using Lactococcus lactis AM99 as a novel Cr（Ⅵ）-reducing bacterium[J]. Journal of Environmental Management，2024，353：120199.
[57]	LI Q，XIONG P P，YANG Q，et al. Adsorption of Cr（Ⅵ）by Escherichia coli[J]. Environmental Science& Technology（China），2014，37（S2）：72-76. 李群，熊攀攀，杨琦，等. 大肠杆菌对铬（Ⅵ）的吸附研究[J]. 环境科学与技术，2014，37（增刊2）：72-76.
[58]	LI Y L，SUN Z Q，MA J，et al. The effect of hydroxyapatite on the adsorption of chromium（Ⅵ）by Escherichia coli[J]. China Water& Wastewater，2022，38（1）：77-82. 李彦霖，孙志强，马军，等. 羟基磷灰石对大肠杆菌吸附铬（Ⅵ）的影响[J]. 中国给水排水，2022，38（1）：77-82.
[59]	VILLAGRASA E，BONET-GARCIA N，SOLÉ A. Ultrastructural evidences for chromium（Ⅲ）immobilization by Escherichia coli K-12 depending on metal concentration and exposure time[J]. Chemosphere，2021，285：131500.
[60]	CHARAN K，MANDAL J，BHATTACHARYYA P. Application of autochthonous extremophilic Bacillus xiamenensis in remediation of groundwater-A sorption-based metal cleaning approach[J]. Groundwater for Sustainable Development，2024，24：101063.
[61]	BROWN J C，MACKAY Q，YU Q，et al. The role of cell surface sulfhydryl and amine binding sites in the removal of Cr（Ⅵ）from solution by Bacillus subtilis bacterial cells[J]. Chemical Geology，2024，664：122299.
[62]	LE Y，HE X，LIU M，et al. Light-driven sustainable enhancement of Cr（Ⅵ）reduction via the combination of Cr（VI）-reducing bacteria，Paraclostridium bifermentans with CdS nanoparticles[J]. Journal of Environmental Chemical Engineering，2023，11（5）：110364.
[63]	CHEN S H，CHEOW Y L，NG S L，et al. Mechanisms for metal removal established via electron microscopy and spectroscopy：a case study on metal tolerant fungi Penicillium simplicissimum[J]. Journal of Hazardous Materials，2019，362：394-402.