枯草芽孢杆菌协同秸秆生物炭对旱地土壤氮矿化效应研究

郑涛; 陈雨晴; 黄显怀; 于雄盛; 周肖瑜; 刘英超; 庄星梅; 方子俊; 钱婧

doi:10.13205/j.hjgc.202608026

枯草芽孢杆菌协同秸秆生物炭对旱地土壤氮矿化效应研究

doi: 10.13205/j.hjgc.202608026

1. 安徽建筑大学环境与能源工程学院环境污染控制与废弃物资源化利用安徽省重点实验室, 合肥 230601;
2. 忻州师范学院, 山西忻州 034000;
3. 湖州市植保检疫与耕肥管理站, 浙江湖州 313000

基金项目:

国家自然科学基金项目（52370001）；环境污染控制与资源再利用、安徽省优秀科研创新团队项目（2022AH010019）；科技创新平台重点科技项目（202305a12020039）；安徽省耕地保护创新示范中心项目（AHGBZX202303）；中国建设水电基础设施局肥西县河流引水工程弃渣场（延长段）临时用地（HYB20240150）；大学生创新创业项目（S202510878087）

详细信息

作者简介:
郑涛（2001—），硕士研究生，主要研究方向为固体废弃物资源化利用。zhengtao1113@foxmail.com

通讯作者:
钱婧（1982—），博士，讲师，主要研究方向为土壤污染治理与改良。anhuiqj2008@163.com

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出版历程
- 收稿日期: 2025-06-12

Nitrogen mineralization effect of Bacillus subtilis combined with straw biochar in dryland soil

1. Anhui Provincial Key of Environmental Pollution Control and Resource Reuse, School of Environment and Energy Engineering, Anhui Jianzhu University, Hefei 230601, China;
2. Xinzhou Normal University, Xinzhou 034000, China;
3. Huzhou Plant Protection & Quarantine and Tillage & Fertilizer Management Station, Huzhou 313000, China

摘要

摘要: 生物炭和微生物菌剂被广泛用于农业土壤改良，为了更好地研究不同种类秸秆生物炭及微生物菌剂单独或配施对旱地土壤氮素转化的影响，设置6组处理进行室内静态培养试验，以确定不同处理对土壤氮素循环的影响。试验结果表明：施加水稻秸秆生物炭使土壤硝态氮含量增加了148.74%~152.68%，显著增强了土壤硝化作用，且配施微生物菌剂后，使土壤平均净氮矿化速率提高了77.28%~99.38%；而油菜秸秆生物炭使土壤硝态氮含量减少了51.66%~57.61%，配施菌剂后，平均净氮矿化速率降低了82.07%~84.73%。4%水稻秸秆生物炭（S）、4%油菜秸秆生物炭（Y）、4%水稻秸秆生物炭+5 mg/kg微生物剂（SJ）和4%油菜秸秆生物炭+5 mg/kg微生物剂（YJ）处理促进了土壤微生物氮（MBN）的合成，S及Y处理较对照组（CK）分别提高2.02，2.20倍，复合处理进一步提高了103.26%~149.44%的MBN含量。由此可见，不同种类秸秆生物炭对土壤硝化作用和净氮矿化作用不一致，复合处理将对MBN产生协同作用；在对旱地土壤综合利用改良中，选择YJ处理为最佳改良方案，可降低土壤无机氮流失风险并增加微生物氮活力。
- 秸秆生物炭 /
- 枯草芽孢杆菌 /
- 旱地土壤 /
- 无机氮 /
- 氮矿化
Abstract: Biochar and microbial inoculants are widely used for agricultural soil amendment. To better investigate the effects of different types of straw-derived biochar and microbial inoculants， applied individually or in combination， on nitrogen transformation in upland soil， this study established six treatment groups for a laboratory-based static incubation experiment， to determine their impacts on soil nitrogen cycle. The experimental results demonstrated that applying rice straw biochar increased the soil nitrate nitrogen content by 148.74% to 152.68%， significantly enhancing soil nitrification. Furthermore， its combined application with microbial inoculants increased the average net soil nitrogen mineralization rate by 77.28% to 99.38%. Conversely， rapeseed straw biochar decreased the soil nitrate nitrogen content by 51.66% to 57.61%， and its combined application with the inoculants reduced the average net nitrogen mineralization rate by 82.07% to 84.73%. Treatments S， Y， SJ， and YJ promoted the synthesis of soil microbial biomass nitrogen （MBN）. Compared with CK， treatments S and Y increased MBN by 2.02- and 2.20-fold， respectively， while the combined treatments （SJ， YJ） further increased the MBN content by 103.26% to 149.44%. Thus， different types of straw biochar exerted inconsistent effects on soil nitrification and net nitrogen mineralization， while the combined treatments exhibited a synergistic effect on MBN production. For the comprehensive improvement and utilization of upland soil， selecting the YJ treatment proved to be the optimal amendment strategy， effectively reducing the risk of soil inorganic nitrogen loss while enhancing microbial nitrogen activity.
- straw biochar /
- Bacillus subtilis /
- dryland soil /
- inorganic nitrogen /
- nitrogen mineralization

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