固定化土著降解菌强化PAHs污染土壤微生物修复研究

刘华清; 刘婷婷; 陈健斌; 柳隋厚; 王艳龙; 成东乐; 张建

doi:10.13205/j.hjgc.202610020

固定化土著降解菌强化PAHs污染土壤微生物修复研究

doi: 10.13205/j.hjgc.202610020

刘华清^1,2,
刘婷婷^1,2,
陈健斌^4,5,
柳隋厚^1,2,
王艳龙^1,2,
成东乐^1,2,
张建^1,2,3, ,

1. 山东科技大学安全与环境工程学院, 山东青岛 266590;
2. 山东科技大学黄河三角洲地表过程与生态完整性研究院, 山东青岛 266590;
3. 山东师范大学地理与环境学院, 山东济南 250358;
4. 山东省黄河三角洲可持续发展研究院, 山东东营 257091;
5. 东营市黄河三角洲生态保护与河口治理重点实验室, 山东东营 257091

基金项目:

国家自然科学基金项目“碳汇型生态净化系统强化去除新污染物的关键技术原理”（52430005）；国家自然科学基金项目“新污染物微生物净化与风险评估”（52622005）；山东省自然科学基金项目“基于稳定同位素联用技术调控POPs在环境中的分子转化过程研究”（ZR2023ZD51）

详细信息

作者简介:
刘华清（1989—），男，教授，主要研究方向为环境微生物。liuhuaqing@sdust.edu.cn

通讯作者:
张建（1976—），男，教授，主要研究方向为污染治理与资源化。zhangjian@sdnu.edu.cn

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出版历程
- 收稿日期: 2026-03-30
- 网络出版日期: 2026-11-10

Enhanced bioremediation of PAH-contaminated soil using immobilized indigenous degrading bacteria

LIU Huaqing^1,2,
LIU Tingting^1,2,
CHEN Jianbin^4,5,
LIU Suihou^1,2,
WANG Yanlong^1,2,
CHENG Dongle^1,2,
ZHANG Jian^{1,2,3
, ,}

1. College of Safety and Environmental Engineering, Shandong University of Science and Technology, Qingdao 266590, China;
2. Institute of Yellow River Delta Earth Surface Processes and Ecological Integravity, Shandong University of Science and Technology, Qingdao 266590, China;
3. School of Geographical Environment, Shandong Normal University, Ji'nan 250358, China;
4. Yellow River Delta Sustainable Development Institute of Shandong Province, Dongying 257091, China;
5. Dongying Key Laboratory of Ecological Protection and Estuary Management of the Yellow River Delta, Dongying 257091, China

摘要

摘要: 针对生物强化污染土壤修复过程中功能菌环境适配性不足的问题，采用单细胞拉曼-重水（D₂O）标记技术，从 PAHs 污染土壤中筛选获得1株土著功能菌 Bacillus sp.（L5），并探究土著功能菌与固定化策略相结合对其在实际污染土壤中修复表现的影响规律。该菌株能够有效降解萘、菲和芘，并使培养体系有机碳含量明显降低。纯培养条件下，外源菌 Stenotrophomonas acidaminiphila （4-1）与 L5 表现出相近的 PAHs 降解能力，因此将其作为对照菌种，2种菌分别结合活性炭、壳聚糖和海藻酸钠制备固定化菌剂，并开展污染土壤微宇宙修复实验，以比较不同菌种及固定化策略下的 PAHs 修复表现。结果显示：L5 在活性炭固定化条件下表现出更明显的修复提升，使萘、菲和芘的降解半衰期较游离土著菌分别缩短至其 24.4%、18.9% 和 27.8%，较活性炭固定化外源菌分别缩短至其23.8%、41.7% 和 23.3%。微生物群落分析显示，固定化处理下相应功能菌属维持了较高的相对丰度，其中活性炭-L5 处理中 Bacillus 属的相对丰度下降幅度较缓，与其较好的 PAHs 修复表现相吻合。综合来看，在研究所比较的菌株及实验条件下，污染土壤来源的土著功能菌 L5 与固定化策略相结合能够有效提升 PAHs 修复效能，表明土著功能菌筛选与固定化技术的结合可为 PAHs 污染土壤高效生物强化修复提供一种可行的技术路径。
- 单细胞拉曼 /
- 固定化 /
- 土著微生物 /
- 生物强化 /
- 土壤修复
Abstract: To address the limited environmental adaptability of functional strains during bioaugmentation of contaminated soil， this study investigated how combining indigenous functional strains with immobilization strategies affects their remediation performance in contaminated soil. Using single-cell Raman spectroscopy coupled with heavy-water （D₂O） labeling， a highly active indigenous strain， Bacillus sp. L5， was isolated from PAH-contaminated soil. Strain L5 effectively degraded naphthalene， phenanthrene， and pyrene， and this degradation was accompanied by a marked decrease in organic carbon in the culture system. Under pure-culture conditions， the exogenous strain Stenotrophomonas acidaminiphila 4-1 exhibited PAH degradation capacities comparable to those of L5， and was therefore used as a reference strain. Both strains were immobilized individually on activated carbon， chitosan， and sodium alginate， followed by soil microcosm experiments to compare PAH remediation performance across different strains and immobilization strategies. Immobilization improved PAH remediation by both strains to varying extents， with activated-carbon-immobilized L5 showing the greatest improvement. The half-lives of naphthalene， phenanthrene， and pyrene were shortened to 24.4%， 18.9%， and 27.8% of those values of free L5， and shortened to 23.8%，41.7%，and 23.3% of those values of the activated-carbon-immobilized strain 4-1， respectively. Microbial community analysis showed that the corresponding functional genera maintained relatively high abundances under immobilized conditions. In particular， the relative abundance of Bacillus declined more slowly in the activated-carbon-L5 treatment， consistent with its superior PAH remediation performance. Overall， under the strains and conditions examined here， combining the indigenous functional strain L5 with immobilization effectively enhanced PAH remediation. These findings demonstrate that integrating screening for indigenous functional strains with immobilization provides a feasible strategy for enhancing the bioaugmentation of PAH-contaminated soil.
- single-cell Raman spectroscopy /
- immobilization /
- indigenous microorganism /
- bioaugmentation /
- soil remediation

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