EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION

HAN Ya-lin; WANG Fu-hao; WANG Qun; LI Ting; SHE Zong-lian

doi:10.13205/j.hjgc.202101007

Volume 39 Issue 1

Apr. 2021

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HAN Ya-lin, WANG Fu-hao, WANG Qun, LI Ting, SHE Zong-lian. EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 51-57,17. doi: 10.13205/j.hjgc.202101007

Citation:

HAN Ya-lin, WANG Fu-hao, WANG Qun, LI Ting, SHE Zong-lian. EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 51-57,17. doi: 10.13205/j.hjgc.202101007

Citation:

HAN Ya-lin, WANG Fu-hao, WANG Qun, LI Ting, SHE Zong-lian. EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(1): 51-57,17. doi: 10.13205/j.hjgc.202101007

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EFFECT OF OPERATIONAL MODE ON NITROGEN REMOVAL AND MICROBIAL CUMMUNITY IN PROCESS OF SIMULTANEOUS PARTIAL NITRIFICATION AND DENITRIFICATION

doi: 10.13205/j.hjgc.202101007

1. Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China;
2. Qingdao Water Group Corporation Limited, Qingdao 266021, China

Received Date: 2019-09-07
Available Online: 2021-04-23

Abstract

Abstract

In this study, simultaneous partial nitrification and denitrification (SPND) process was successfully established for the treatment of high-salinity wastewater in a hybrid sequencing batch biofilm reactor (HSBBR). The effect of operational mode on the reactor performance and microbial community was investigated. When the reactor was operated in alternating anoxic/aerobic mode (anoxic/aerobic hour ratio was 2.0 h/4.5 h), the removal efficiencies of NH₄⁺-N, total inorganic nitrogen (TIN) and COD were 95.00%, 84.83% and 86.72%, respectively, and the effluent contained nitrite and nitrate. When operational mode was switched to fully aerobic mode (anoxic/aerobic hour ratio was 0.0/6.5 h), removal efficiencies of NH₃-N, TIN and COD increased to 100.00%, 85.94% and 89.46%, and nitrate was the only nitrogen component in the effluent. The high-throughput sequencing results showed that the dominant phyla were Proteobacteria and Bacteroidetes in both suspended sludge (S-sludge) and biofilm in two modes. Genus Nitrosomonas was the only ammonia-oxidizing bacteria (AOB) detected in this study and appeared higher relative abundance in S-sludge and biofilm samples in the fully aerobic mode compared to the alternating anoxic/aerobic mode. S-sludge and biofilm shared similar dominant denitrifying bacteria (DNB), including genera Candidatus_Competibacter, Paracoccus, Thauera and Denittrasoma. The relative abundance of Candidatus_Competibacter and Thauera decreased in fully aerobic mode, while Paracoccus and Denitratisoma showed increasing tendency. Multiple DNB genera accompanied by AOB contributed to the efficient nitrogen removal via SPND process.
- high salinity,
- hybrid sequencing batch biofilm reactor (HSBBR),
- operation mode,
- simultaneous partial nitrification and denitrification,
- microbial community

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References(39)

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