EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS

WU Dai-shun; CHANG Huan-huan; CHEN Cui-zhong; YANG Hao; HOU Hong-xun; SUN Hong-wei

doi:10.13205/j.hjgc.202103012

Volume 39 Issue 3

Jul. 2021

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WU Dai-shun, CHANG Huan-huan, CHEN Cui-zhong, YANG Hao, HOU Hong-xun, SUN Hong-wei. EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012

Citation:

WU Dai-shun, CHANG Huan-huan, CHEN Cui-zhong, YANG Hao, HOU Hong-xun, SUN Hong-wei. EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012

Citation:

WU Dai-shun, CHANG Huan-huan, CHEN Cui-zhong, YANG Hao, HOU Hong-xun, SUN Hong-wei. EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(3): 82-89. doi: 10.13205/j.hjgc.202103012

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EFFECTS OF FREE AMMONIA (FA) ON STRUCTURE AND DIVERSITY OF MICROFLORA IN SBR SHORT-CUT NITRIFICATION PROCESS

doi: 10.13205/j.hjgc.202103012

1. College of Marine and Biochemical Engineering, Fujian Provincial Key Laboratory of Coastal Basin Environmental Measurement and Control, Fujian Normal University, Fuqing 350300, China;
2. College of Environmental and Municipal Engineering, Lanzhou Jiaotong University, Lanzhou 730070, China;
3. Anhui Guozhen Environmental Protection and Energy Saving Technology Co., Ltd, Hefei 230031, China;
4. College of Environmental and Material Engineering, Yantai University, Yantai 264005, China

Received Date: 2020-10-15
Available Online: 2021-07-19

Abstract

Abstract

Based on 16S rRNA genes-Illumina MiSeq high-throughput sequencing, this study was aimed to investigate the community composition and structural characteristics of microorganisms under different free ammonia (FA) concentrations (0.5, 5, 10, 15 mg/L), and it was settled in four parallel laboratory-scale sequencing batch reactors (SBRs, denoted as R_0.5, R₅, R₁₀ and R₁₅) to better understand how FA concentrations influenced the nitrification. The results showed that FA could significantly affect the composition, population structure and function of the microbial community in the system. The diversity index(including Chao1, ACE, Shannon and Simpson index) of R_0.5 was the largest among the four groups of reactors, indicating that species diversity of R_0.5 was the highest, while the lowest was achieved in R₁₅. In addition, Proteobacteria (45.9%~70.5%) and Bacteroidetes (11.8%~41.3%) were found to be major groups with higher relative abundance at the phylum level, and the relative abundance of Proteobacteria increased with the increase of FA concentration. At the microbial level, Zoogloea and Thauera were the dominant generas, and Nitrosomonas and Nitrospira had significantly higher abundance in R₁₀ than the other systems. A total of 25 groups of microorganisms with significant differences were obtained based on LEfSe analysis, thereby key biomarkers of the microflora were obtained at the microbiological classification level, under each FA concentration.
- free ammonia (FA),
- nitrite oxidizing bacteria,
- high-throughput sequencing technology,
- community composition,
- LEfSe analysis

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References(20)

References

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