EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING

ZHANG Ai-sheng; QU Jun-rong; XU Bin; YANG Xiao-wei; SHAO Yan-qiu; FU Yan-li; LI Bo-en; ZHU Ying

doi:10.13205/j.hjgc.202105014

Volume 39 Issue 5

Jan. 2022

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ZHANG Ai-sheng, QU Jun-rong, XU Bin, YANG Xiao-wei, SHAO Yan-qiu, FU Yan-li, LI Bo-en, ZHU Ying. EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(5): 104-110. doi: 10.13205/j.hjgc.202105014

Citation:

ZHANG Ai-sheng, QU Jun-rong, XU Bin, YANG Xiao-wei, SHAO Yan-qiu, FU Yan-li, LI Bo-en, ZHU Ying. EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(5): 104-110. doi: 10.13205/j.hjgc.202105014

Citation:

ZHANG Ai-sheng, QU Jun-rong, XU Bin, YANG Xiao-wei, SHAO Yan-qiu, FU Yan-li, LI Bo-en, ZHU Ying. EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(5): 104-110. doi: 10.13205/j.hjgc.202105014

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EFFECTS OF AMBIENT TEMPERATURE ON ANTIBIOTIC RESISTANCE GENES IN SLUDGE COMPOSTING

doi: 10.13205/j.hjgc.202105014

1. Institute of New Materials, Qilu University of Technology(Shandong Academy of Sciences), Jinan 250014, China;
2. Tsingtao Loushanhe Water Resources Co., Ltd, Qingdao 266000, China;
3. Tsingtao Tuandao Sewage Treatment Plant, Qingdao 266000, China

Received Date: 2020-07-22
Available Online: 2022-01-17

Abstract

Abstract

To study the effect of ambient temperature on the changes of antibiotic resistance gene variation in sludge composting process, municipal sewage treatment plant sludge dewatering, peanut shells and compost backmixing material were selected as composting materials. The effects of the three external environment temperature on changes of antibiotic resistances genes in sludge composting process were analyzed and compared by measuring the physical and chemical properties of composted materials and resistance genes(tetA、tetQ、sul2、sul3) and class integron(IntI1) abundance in the composting process at 30℃, 50℃ and 55℃ by the indoor static composting and turning method. The results showed that the abundance of tetA, tetQ, sul2 and sul3 resistance genes in the process of sludge composting at ambient temperatures outside 50℃ and 55℃ could be effectively reduced, and the degradation rate of total ARGs reached 66%~83% at the end of composting, while the degradation rate of total ARGs was about 30% at the end of composting at external temperature of 30℃. The degradation rate of mobile genetic elements IntI1 at the end of the composting at 50℃ and 55℃ was 92% above, higher than that of at 30℃ (71.9%). The reason may be that proper high ambient temperature inhibited the growth and metabolism of some microbial in the pile and reduced the spread of IntI1, and then affected the spread of the ARGs. The compost at ambient temperature of 30℃ reached maturity on the 36th day, and the germination index was 59.80%. The compost at 50℃ and 55℃ reached maturity on the 28th day, the germination index reached 60%, and the maturity rate was significantly higher than that at 30℃. The study showed that composting conditions at ambient temperatures of 50℃ and 55℃ could shorten the time required for composting compared with that of 30℃, and had a good degradation effect on tetA, tetQ, sul2, sul3 resistance genes and IntI1 abundance. Therefore, appropriately increasing the ambient temperature during the composting process had certain effect on sludge fermentation and ARGs.
- sludge composting,
- antibiotic resistance genes,
- environment temperature

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References(16)

References

[1]	BRJESSON S, MELIN S, MATUSSEK A, et al. A seasonal study of the mecA gene and Staphylococcus aureus including methicillin-resistant S. aureus in a municipal wastewater treatment plant[J]. Water Research, 2008, 43(15):3900.
[2]	徐冰洁, 罗义, 周启星, 等. 抗生素抗性基因在环境中的来源、传播扩散及生态风险[J]. 环境化学, 2010, 29(2):169-178.
[3]	COLOMER-LLUCH M, CALERO-C CERES W, JEBRI S, et al. Antibiotic resistance genes in bacterial and bacteriophage fractions of Tunisian and Spanish wastewaters as markers to compare the antibiotic resistance patterns in each population[J]. Environment International, 2014, 73:167-175.
[4]	HEUER H, SCHMITT H, SMALLA K. Antibiotic resistance gene spread due to manure application on agricultural fields[J]. Current Opinion in Microbiology, 2011, 14(3):236-243.
[5]	杨凤霞, 毛大庆, 罗义, 等. 环境中抗生素抗性基因的水平传播扩散[J]. 应用生态学报, 2013, 24(10):2993-3002.
[6]	李兴盛. 城市污水污泥的堆肥化处理与资源化利用[G]//四川省循环经济发展模式(1)-污泥再生利用,四川省环境科学学会专题资料汇编,2005.
[7]	杨瑶, 徐鹤, 尹建锋. 我国污水处理厂污泥资源化利用现状分析[J]. 南水北调与水利科技, 2012, 10(5):114-118.
[8]	DU, SHIN, WANG, et al. Effect of temperature on the expression of genes related to the accumulation of chlorophylls and carotenoids in albino tea[J]. The Journal of Horticultural Science and Biotechnology, 2009, 84(3):365-369.
[9]	韦蓓, 黄福义, 李虎, 等. 污泥堆肥过程中磺胺类和大环内酯类抗性基因的残留[J]. 应用与环境生物学报, 2014, 20(3):395-400.
[10]	卢晓梅. 超高温堆肥微生物群落特征及其消减污泥抗性基因研究[D]. 福州:福建农林大学, 2018.
[11]	MA ZHANG, HWANG. Research and development of a semi-hermetic reciprocating compressor for transcritical CO₂ refrigeration cycle[J]. Proceedings of the Institution of Mechanical Engineers, 2011, 225(1):.
[12]	KIM J, PARK C, KIM T H, et al. Effects of various pretreatments for enhanced anaerobic digestion with waste activated sludge[J]. Journal of Bioscience and Bioengineering, 2003, 95(3):271-275.
[13]	郁红艳, 曾光明, 胡天觉, 等. 真菌降解木质素研究进展及在好氧堆肥中的研究展望[J]. 中国生物工程杂志, 2003,23(10):57-61.
[14]	IANNOTTI D A, PANG T, TOTH B L, et al. A Quantitative Respirometric Method for Monitoring Compost Stability. 2013, 1(3):52-65.
[15]	曹刚,李红旭,赵明新,等.微生物菌剂浓度对梨树废弃枝条堆肥化处理的影响[J].甘肃农业科技,2017(12):43-46.
[16]	张园,耿春女,何承文,等.堆肥过程中有机质和微生物群落的动态变化[J].生态环境学报,2011,20(11):1745-1752.