DETECTION OF WATERBORNE PATHOGENS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)

WU Xiao-jin; LIU Gang; HU Hong-ying; LU Yun

doi:10.13205/j.hjgc.202112002

Volume 39 Issue 12

Mar. 2022

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WU Xiao-jin, LIU Gang, HU Hong-ying, LU Yun. DETECTION OF WATERBORNE PATHOGENS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(12): 10-17,37. doi: 10.13205/j.hjgc.202112002

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WU Xiao-jin, LIU Gang, HU Hong-ying, LU Yun. DETECTION OF WATERBORNE PATHOGENS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(12): 10-17,37. doi: 10.13205/j.hjgc.202112002

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DETECTION OF WATERBORNE PATHOGENS BY LOOP-MEDIATED ISOTHERMAL AMPLIFICATION (LAMP)

doi: 10.13205/j.hjgc.202112002

State Key Laboratory of Environmental Simulation and Pollution Control & State Environment Protection Key Laboratory of Microorganism Application and Safety Control, School of Environment, Tsinghua University, Beijing 100084, China

Received Date: 2020-07-05
Available Online: 2022-03-30
Publish Date: 2022-03-30

Abstract

Abstract

Microbiological index is important to water safety, while the measurement of pathogenic bacteria in water is not included in the water quality monitoring due to the technique barrier. Most water quality standards contain only indicator microorganisms. However, the correlation between these indicators and real pathogens is not good enough to guarantee the safety. Therefore, developing fast and simple pathogen detection technologies is the key to improve microbiological monitoring in water. Loop-mediated isothermal amplification is a fast PCR technology to detect DNA independent of expensive PCR machine. This study attempted to detect 8 prevalent indicators and pathogenic bacteria: total coliform, Salmonella enterica, Shigella flexneri, Escherichia coli O157∶H7, Yesinia enterocolitica, Legionella pneumophila, Pseudomonas aeruginosa, Mycobacterium tuberculosis, by LAMP. The primers and PCR conditions of five of them were successfully optimized. The sensitivity of LAMP was to detect 100 gene copies within 45 minutes, and this technology can be applied in the detection of pathogens in raw wastewater.
- water quality index,
- pathogenic bacterial,
- isothermal PCR,
- loop-mediated isothermal amplification(LAMP)

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References(58)

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