ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE

XIE Mingde; FENG Mei; TANG Yiming; YI Xiaoying; LIU Dan

doi:10.13205/j.hjgc.202202007

Volume 40 Issue 2

Apr. 2022

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XIE Mingde, FENG Mei, TANG Yiming, YI Xiaoying, LIU Dan. ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 42-46. doi: 10.13205/j.hjgc.202202007

Citation:

XIE Mingde, FENG Mei, TANG Yiming, YI Xiaoying, LIU Dan. ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(2): 42-46. doi: 10.13205/j.hjgc.202202007

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ANALYSIS ON MICROBIAL COMMUNITY STRUCTURES AND DIVERSITY IN AGED REFUSE

doi: 10.13205/j.hjgc.202202007

1. School of Life Science and Engineering, Southwest Jiaotong University, Chengdu 611756, China;
2. Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu 611756, China

Received Date: 2020-11-19
Available Online: 2022-04-02
Publish Date: 2022-04-02

Abstract

Abstract

The microbiota contained in the aged refuse plays an important role in the treatment of leachate. In order to understand the effect of microbial community on the degradation of organic matters in leachate, 16 S rRNA gene high-throughput sequencing technology and analysis method were used to study the microbial community structure and diversity of aged refuse at different sites. The results showed that the aged refuse contained rich microbial communities, and the richness of each site was similar, but the diversity was different; each site contained Firmicutes, Euryarchaeota, Chloroflexi, Bacteroidetes, Synergistetes, Actinobacteria, Proteobacteria, Planctomycetes, Thermotogae and Atribacteria(with relative abundance of 1.0% above); Firmicutes, the dominant phyla, had the highest relative abundance at K1, K2, K3 and K4, which were 39.10%, 31.79%, 47.09% and 33.84%, respectively; the relative abundance of Euryarchaeota was higher at K1 and K2 with higher organic load of leachate and at K4 with longer hydraulic retention time, while the relative abundance was lower at K3 in the middle layer. The structure and diversity of the microbial community were related to the leachate loading, and the diversity of the microbial community was higher in the aged refuse layer with higher leachate loading. The rich microbial community in the aged refuse layer played an important role in stabilizing the physical and chemical properties of the leachate.
- aged refuse,
- leachate,
- microbial community,
- diversity

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References(26)

References

[1]	FLEMING I R,ROWE R K.Laboratory studies of clogging of landfill leachate collection and drainage systems[J].Canadian Geotechnical Journal,2004,41:134-153.
[2]	VANGULCK J F,RITTMANN B E,Cooke A J.Predicting biogeochemical calcium precipitation in landfill leachate collection systems[J].Biodegradation,2003,14(5):331-346.
[3]	陈云敏,兰吉武,李育超,等.垃圾填埋场渗沥液水位壅高及工程控制[J].岩石力学与工程学报,2014,33(1):154-163.
[4]	REAGAN M,ROWE R K.Clogging of gravel drainage layers permeated with landfill leachate[J].Journal of Geotechnical and Geoenvironmental Engineering,2007,133 (8):1026-1039.
[5]	ROWE R K,CULLIMORE D R,ARMSTRONG M D.Mass loading and the rate of clogging due to municipal solid waste leachate[J].Canadian Geotechnical Journal,2000,37(2):355-370.
[6]	石磊,张全,牛冬杰,等.矿化垃圾反应床处理渗滤液的微生物学特性[J].同济大学学报(自然科学版),2007,35(8):1085-1089.
[7]	赵由才,柴晓利,牛冬杰.矿化垃圾基本特性研究[J].同济大学学报(自然科学版),2006,34(10):1360-1364.
[8]	边炳鑫,赵由才,周正,等.矿化垃圾生物反应床处理渗滤液技术[J].环境工程,2007,25(1):52-56.
[9]	张华,赵由才.生活垃圾填埋场中矿化垃圾的综合利用[J].山东建筑工程学院学报,2004,19(3):46-51.
[10]	何岩.矿化垃圾反应床处理填埋场渗滤液工艺优化及运行机制研究[D].上海:同济大学,2008.
[11]	谢明德,唐一鸣,冯梅,等.利用矿化垃圾层预防和控制渗滤液导排系统堵塞[J].环境化学,2021,40(2):614-623.
[12]	CHEN S F,ZHOU Y Q,CHEN Y R,et al.FASTP:an ultra-fast all-in-one FASTQ preprocessor[J].Bioinformatics,2018,34(17):i884-i890.[13] MAGOČ T, SALZBERG S L. FLASH:fast length adjustment of short reads to improve genome assemblies[J].Bioinformatics,2011,27(21):2957-2963.
[14]	EDGAR R C.UPARSE:highly accurate OTU sequences from microbial amplicon reads[J].Nat Methods,2013,10(10):996-998.
[15]	STACKEBRANDT E,GOEBEL B M.Taxonomic Note:a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology[J].International Journal of Systematic Bacteriology,1994,44(4):846-849.
[16]	WANG Q,GARRITY G M,TIEDJE J M,et al.Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J].Applied and Environmental Microbiology,2007,73(16):5261-5267.
[17]	SHU D T,HE Y,YUE H,et al.Metagenomic insights into the effects of volatile fatty acids on microbial community structures and functional genes in organotrophic anammox process[J].Bioresource Technology,2015,196:621-633.
[18]	赵诗惠,吕亮,蒋志云,等.ABR-MBR组合工艺短程硝化过程的微生物种群[J].中国环境科学,2018,38(2):566-573.
[19]	PAN F,XU A H,XIA D S,et al.Effects of octahedral molecular sieve on treatment performance,microbial metabolism,and microbial community in expanded granular sludge bed reactor[J].Water Research,2015,87:127-136.
[20]	王宝山,温成成,孙秦川,等.石油烃类污染对青藏高原北麓河地区冻区土壤微生物多样性的影响[J].环境工程学报,2018,12(10):2917-2928.
[21]	BAREITHER C A,WOLFE G L,MCMAHON K D,et al.Microbial diversity and dynamics during methane production from municipal solid waste[J].Waste Management,2013,33(10):1982-1992.
[22]	LEONG L,DENMAN S,HUGENHOLTZ P,et al.Amino acid and peptide utilization profiles of the fluoroacetate-degrading bacterium synergistetes strain MFA1 under varying conditions[J].Microbial Ecology,2016,71(2):494-504.
[23]	ANA I D,PAULA O,ADRIANA L,et al.Metagenomic analysis of bacterial communities from a nitrification-denitrification treatment of landfill leachates[J].CLEAN:Soil,Air,Water,2019,47(11):1-22.
[24]	JAENICKE S,ANDER C,BEKEL T,et al.Comparative and joint analysis of two metagenomic datasets from a biogas fermenter obtained by 454-pyrosequencing[J].PLoS One,2011,6(1):e14519.
[25]	闫冰,夏嵩,桂双林,等.厌氧氨氧化菌富集培养过程微生物群落结构及多样性[J].环境科学,2020,41(12):5535-5543.
[26]	BOVIO P,CABEZAS A,ETCHEBEHERE C.Preliminary analysis of Chloroflexi populations in full scale UASB methanogenic reactors[J].Journal of Applied Microbiology,2019,126(2):667-683.
[27]	孟晓山,张玉秀,隋倩雯,等.氨氮浓度对猪粪厌氧消化及产甲烷菌群结构的影响[J].环境工程学报,2018,12(8):2346-2356.