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Volume 40 Issue 5
Jul.  2022
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KONG Jia, SHEN Bo-xiong, KONG Wen-wen, DAN Kai-xuan. EXPERIMENTAL STUDY ON CO2 FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 9-17,94. doi: 10.13205/j.hjgc.202205002
Citation: KONG Jia, SHEN Bo-xiong, KONG Wen-wen, DAN Kai-xuan. EXPERIMENTAL STUDY ON CO2 FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 9-17,94. doi: 10.13205/j.hjgc.202205002

EXPERIMENTAL STUDY ON CO2 FIXATION COUPLED WITH WASTEWATER PURIFICATION BY CHLORELLA VULGARIS UNDER DIFFERENT AMMONIUM CONCENTRATIONS

doi: 10.13205/j.hjgc.202205002
  • Received Date: 2021-07-06
    Available Online: 2022-07-02
  • To explore the potential of Chlorella vulgaris(C. vulgaris) in fixing CO2, purifying wastewater and producing protein simultaneously, the effects of different ammonium concentrations(30 mg/L, 60 mg/L, 90 mg/L NH4Cl) and CO2 concentrations(0.038% and 10%) on the growth, CO2 fixation, nutrients removal and protein production of C. vulgaris were studied. The combination of Logistic function and the modified Monod function was also used to describe the relationship between C. vulgaris specific growth rate and concentration of ammonium(NH+4-N) and phosphate(PO3-4-P), respectively.Resultsshowed that the biomass of 10% CO2 group(380.16~499.52 mg/L) was 3.54~8.30 times that of 0.038% CO2 group(44.73~120.00 mg/L). Meanwhile, the consumption rates of ammonium and phosphate in the 10% CO2 group were significantly higher than those in the 0.038% CO2 group. Correlation analysis showed that specific growth rate, carbon fixation rate, protein were all positively correlated with C. vulgaris biomass(R2>0.86, P<0.05). With 10% CO2 and 60 mg/L NH4Cl, the maximum values of specific growth rate, carbon fixation rate, and protein were 0.21 d-1, 42.62 mg/(L·d), and 228.43 mg/L, respectively. In addition, the fitting results indicated that the combination of Logistic function and the modified Monod function could well describe C. vulgaris growth(correlation coefficient R2 is 0.39~0.96), and nutrients were more easily utilized by C. vulgaris at 10% CO2. This experiment could provide a theoretical reference for fixing CO2, purifying wastewater and producing protein simultaneously by microalgae.
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