SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY

HE Wen-shan; ZHANG Ru; LI Si-qi; LI Wen-jin; SONG Zhe-hua; PENG Guang; SHEN Peng; WANG Xiao-hui

doi:10.13205/j.hjgc.202205008

Volume 40 Issue 5

Jul. 2022

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HE Wen-shan, ZHANG Ru, LI Si-qi, LI Wen-jin, SONG Zhe-hua, PENG Guang, SHEN Peng, WANG Xiao-hui. SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 53-58,66. doi: 10.13205/j.hjgc.202205008

Citation:

HE Wen-shan, ZHANG Ru, LI Si-qi, LI Wen-jin, SONG Zhe-hua, PENG Guang, SHEN Peng, WANG Xiao-hui. SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 53-58,66. doi: 10.13205/j.hjgc.202205008

Citation:

HE Wen-shan, ZHANG Ru, LI Si-qi, LI Wen-jin, SONG Zhe-hua, PENG Guang, SHEN Peng, WANG Xiao-hui. SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(5): 53-58,66. doi: 10.13205/j.hjgc.202205008

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SCREENING OF A CHLORTETRACYCLINE-DEGRADING STRAIN AND ITS DEGRADATION CONDITIONS OPTIMIZATION USING RESPONSE SURFACE METHODOLOGY

doi: 10.13205/j.hjgc.202205008

1. Beijing Water Treatment Environmental Protection Material Engineering Technology Research Center, Beijing University of Chemical Technology, Beijing 100029, China;
2. BESG New-Energy Environmental Engineering Co., Ltd, Beijing 100012, China;
3. Zhumadian Huazhong Chia Tai Co., Ltd., Zhumadian 463023, China;
4. Chinese Research Academy of Environmental Sciences, Beijing 100012, China

Received Date: 2021-09-01
Available Online: 2022-07-02

Abstract

Abstract

A chlortetracycline-degrading strain was isolated from the activated sludge of a chlortetracycline pharmaceutical factory in Zhumadian in Henan. Based on the analysis of its 16S rDNA gene sequence, the isolated strain named JMS-B01 was identified as a member of the genus Bacillus. To improve the chlortetracycline degradation rate, the degradation conditions were optimized. Firstly, three factors playing important roles in the chlortetracycline degradation rate were selected based on the orthogonal test. Then box-Behnken experimental design and response surface method were used to analyze the optimal degradation conditions. The fitting curve had a good correlation with the measured values. The optimal conditions were as follows:temperature of 34.0℃, chlortetracycline concentration of 102.5 mg/L, inoculation amount of 2%(V/V). Under the optimal conditions mentioned above, the chlortetracycline degradation rate reached 96.50%. Temperature was one of the most important factors affecting the chlortetracycline degradation rate of strain JMS-B01.
- chlortetracycline,
- biodegradation,
- box-Behnken,
- response surface methodology

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References(28)

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