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Volume 40 Issue 8
Nov.  2022
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Article Contents
GENG Ziqian, DAI Wenting, LI Chao, TANG Jie, DAI Kun, CENG Jianxiong, ZHANG Fang. ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(8): 90-95. doi: 10.13205/j.hjgc.202208012
Citation: GENG Ziqian, DAI Wenting, LI Chao, TANG Jie, DAI Kun, CENG Jianxiong, ZHANG Fang. ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM[J]. ENVIRONMENTAL ENGINEERING , 2022, 40(8): 90-95. doi: 10.13205/j.hjgc.202208012

ENHANCED METHANOGENESIS OF WASTE ACTIVATED SLUDGE FERMENTATION BY DOSING AN ALGINATE-DEGRADING CONSORTIUM

doi: 10.13205/j.hjgc.202208012
  • Received Date: 2021-10-01
  • Publish Date: 2022-11-08
  • Methane production by anaerobic digestion is popular biotechnology to utilize the wanted waste activated sludge (WAS). But the hydrolysis is known as the rate-limiting step of WAS fermentation. The uronic acids (such as alginate and polygalacturonic acid) in WAS can form hydrogels with cationic ions (i.e. Ca2+) in wastewater, which can maintain sludge structure and retard the microbial hydrolysis. Thus, these organics are deemed as the main components of structural extracellular polymeric substances. In this work, an alginate-degrading consortium (ADC) with high activity was enriched in a mesophilic chemostat using alginate as the substrate. The results showed that the enriched ADC could notably enhance methane production from WAS. The methane yield increased by 53.6% under a low ratio of inoculum to WAS of 0.03 g/g [m(ADC)∶m(VSS)]. Moreover, the typical organic components of WAS, including polygalacturonic acid, dextran, and casein, could be utilized by the enriched ADC. The genus Bacteroides (96.3%) was identified as the dominant bacteria in ADC by an Illumina Miseq high-throughput sequencing. Therefore, this work provided a new microbial method for biochemical production in WAS.
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