FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF <i>RHODOCOCCUS ERYTHROPOLIS</i> KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS

CHEN Shiyuan; CHEN Jixiang; WANG Yonggang; YANG Yanwu; ZHANG Bowen

doi:10.13205/j.hjgc.202401024

Volume 42 Issue 1

Jan. 2024

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2024 > 42(1): 184-190

CHEN Shiyuan, CHEN Jixiang, WANG Yonggang, YANG Yanwu, ZHANG Bowen. FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF RHODOCOCCUS ERYTHROPOLIS KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(1): 184-190. doi: 10.13205/j.hjgc.202401024

Citation:

CHEN Shiyuan, CHEN Jixiang, WANG Yonggang, YANG Yanwu, ZHANG Bowen. FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF RHODOCOCCUS ERYTHROPOLIS KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(1): 184-190. doi: 10.13205/j.hjgc.202401024

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FORMATION OF VIABLE BUT NON-CULTURABLE STATE OF RHODOCOCCUS ERYTHROPOLIS KB1 INDUCED BY HIGH CONCENTRATION BENZENE AND CHARACTERISTICS ITS RESUSCITATED CELLS

doi: 10.13205/j.hjgc.202401024

1. School of Petrochemical Engineering, Lanzhou University of Technology, Lanzhou 730050, China;
2. School of Life Science and Engineering, Lanzhou University of Technology, Lanzhou 730050, China

Received Date: 2022-11-13
Available Online: 2024-04-29

Abstract

Abstract

To study the inducing effects of different concentrations of benzene on the oil-degrading bacterium R. erythropolis KB1, characterize the benzene-induced VBNC state of the bacterial cells, and physiological and biochemical characteristics of bacterial cells, and petroleum degradation ability of the resuscitated R. erythropolis cells, R. erythropolis KB1 was cultured in LB medium and basal medium. The formation of the VBNC state of R. erythropolis KB1 cells induced by benzene was observed by spectrophotometry, scanning electron microscopy, and transmission electron microscopy, and the oil degradation abilities of the resuscitated bacterial cells were determined by infrared spectrophotometry. The results showed that when the concentration of benzene in the culture medium was 2.5% above, the growth of R. erythropolis KB1 could be effectively inhibited. After being cultured for 6 h, R. erythropolis KB1 cells entered the VBNC state. The VBNC cells could be resuscitated into the culturable state under certain conditions. The resuscitated bacterial cells showed good adaptabilities to the environments, and their oil degradation ability was not changed obviously.
- Rhodococcus,
- benzene,
- VBNC,
- resuscitation

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References(30)

References

[1]	孔德辉,刘东,郭书海,等.石油污染土壤热解修复反应规律与机制研究.[J].环境科学学报,2021,41 (8):3339-3348.
[2]	郑瑾,付雅丽,宋权威,等.微生物强化修复石油污染土壤的研究进展.[J].生物工程学报,2021,37(10):3622-3635.
[3]	OSSAI I C,AHMED A,HASSAN A,et al.Remediation of soil and water contaminated with petroleum hydrocarbon:a review[J].Environmental Technology & Innovation,2020,17:100526.
[4]	LIU H,YANG G,JIA H,et al.Impact of long-term cultivation with crude oil on wetland microbial community shifts and the hydrocarbon degradation potential[J].Energy Sources,Part A:Recovery,Utilization,and Environmental Effects,2021:1-13.
[5]	ZHU X.Application of microbial remediation in the treatment of offshore oil pollution;proceedings of the IOP conference series:earth and environmental science,F[C].IOP Publishing,2021,781(5):052008.
[6]	ULRICH N,NAGLER K,LAUE M,et al.Experimental studies addressing the longevity of Bacillus subtilis spores-The first data from a 500-year experiment[J].PLoS one,2018,13(12):e0208425.
[7]	ZHANG X,AHMAD W,ZHU X,et al.Viable but nonculturable bacteria and their resuscitation:implications for cultivating uncultured marine microorganisms[J].Marine Life Science & Technology,2021,3(2):189-203.
[8]	DAâSSI D,QABIL ALMAGHRIBI F.Petroleum-contaminated soil:environmental occurrence and remediation strategies[J].3 Biotech,2022,12(6):1-17.
[9]	GENG S,XU G,YOU Y,et al.Occurrence of polycyclic aromatic compounds and interdomain microbial communities in oilfield soils[J].Environmental Research,2022,212:113191.
[10]	ALVAREZ P J,ANID P J,VOGEL T M.Kinetics of aerobic biodegradation of benzene and toluene in sandy aquifer material[J].Biodegradation,1991,2(1):43-51.
[11]	FAHY A,BALL A S,LETHBRIDGE G,et al.High benzene concentrations can favor Gram-positive bacteria in groundwaters from a contaminated aquifer[J].FEMS Microbiology Ecology,2008,65(3):526-533.
[12]	WEI Y,CHEN J,WANG Y,et al.Bioremediation of the petroleum contaminated desert steppe soil with Rhodococcus erythropolis KB1 and Its effect on the bacterial communities of the soils[J].Geomicrobiology Journal,2021,38(10):842-849.
[13]	DING T,XUAN X T,LI J,et al.Disinfection efficacy and mechanism of slightly acidic electrolyzed water on Staphylococcus aureus in pure culture[J].Food Control,2016,60:505-510.
[14]	ZHANG L,JIANG L,NIE X,et al.Factors affecting the occurrence of viable but non-culturable state in Salmonella,Escherichia coli and Staphylococcus aureus during thermosonication and prevent it with sodium pyruvate[J].International Journal of Food Science & Technology,2021,56(5):2244-2254.
[15]	DONG K,PAN H,YANG D,et al.Induction,detection,formation,and resuscitation of viable but non-culturable state microorganisms[J].Comprehensive Reviews in Food Science and Food Safety,2020,19(1):149-183.
[16]	LIN H,YE C,CHEN S,et al.Viable but non-culturable E.coli induced by low level chlorination have higher persistence to antibiotics than their culturable counterparts[J].Environmental Pollution,2017,230:242-249.
[17]	MAERTENS L,MATROULE J Y,VAN HOUDT R.Characteristics of the copper-induced viable-but-non-culturable state in bacteria[J].World Journal of Microbiology and Biotechnology,2021,37(3):1-9.
[18]	MORISHIGE Y,FUJIMORI K,AMANO F.Use of flow cytometry for quantitative analysis of metabolism of viable but non-culturable (VBNC) Salmonella[J].Biological and Pharmaceutical Bulletin,2015:38:1255-64.
[19]	CHEN S,LI X,WANG Y,et al.Induction of Escherichia coli into a VBNC state through chlorination/chloramination and differences in characteristics of the bacterium between states[J].Water Research,2018,142:279-288.
[20]	SHAHRYARI S,ZAHIRI H S,HAGHBEEN K,et al.High phenol degradation capacity of a newly characterized Acinetobacter sp.SA01:bacterial cell viability and membrane impairment in respect to the phenol toxicity[J].Ecotoxicology and Environmental Safety,2018,164:455-466.
[21]	XIE M,XU L,ZHANG R,et al.Viable but nonculturable state of yeast Candida sp.strain LN1 induced by high phenol concentrations[J].Applied and Environmental Microbiology,2021,87(18):e01110-e01121.
[22]	FIDA T T,MORENO-FORERO S K,HEIPIEPER H J,et al.Physiological and transcriptome response of the polycyclic aromatic hydrocarbon degrading Novosphingobium sp.LH128 after inoculation in soil[J].Environ Sci Technol 2017,51:1570-1579.
[23]	PAWLOWSKI D R,METZGER D J,RASLAWSKY A,et al.Entry of Yersinia pestis into the viable but nonculturable state in a low-temperature tap water microcosm[J].PLoS One,2011,6(3):e17585.
[24]	FAN J,JIA Y,XU D,et al.Anaerobic condition induces a viable but nonculturable state of the PCB-degrading Bacteria Rhodococcus biphenylivorans TG9[J].Science of the Total Environment,2021,764:142849.
[25]	郑玉玺,黄利华,贾强,等.次氯酸钠诱导肠炎沙门氏菌活的非可培养状态形成及复苏过程研究.[J].食品安全质量检测学报,2021,12(14):5546-5552.
[26]	ZHANG J,WANG L,SHI L,et al.Survival strategy of Cronobacter sakazakii against ampicillin pressure:induction of the viable but nonculturable state[J].International Journal of Food Microbiology,2020,334:108819.
[27]	PINTO D,ALMEIDA V,ALMEIDA SANTOS M,et al.Resuscitation of Escherichia coli VBNC cells depends on a variety of environmental or chemical stimuli[J].Journal of Applied Microbiology,2011,110(6):1601-1611.
[28]	SUN F,CHEN J,ZHONG L,et al.Characterization and virulence retention of viable but nonculturable Vibrio harveyi[J].FEMS Microbiology Ecology,2008,64(1):37-44.
[29]	SU X,ZHANG S,MEI R,et al.Resuscitation of viable but non-culturable bacteria to enhance the cellulose-degrading capability of bacterial community in composting[J].Microbial Biotechnology,2018,11(3):527-536.
[30]	SU X,LI S,XIE M,et al.Enhancement of polychlorinated biphenyl biodegradation by resuscitation promoting factor (Rpf) and Rpf-responsive bacterial community[J].Chemosphere,2021,263:128283.