CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS

ZHOU Qi; HAN Peipei; HOU Yanan; HUANG Cong

doi:10.13205/j.hjgc.202403006

Volume 42 Issue 3

Mar. 2024

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ZHOU Qi, HAN Peipei, HOU Yanan, HUANG Cong. CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(3): 51-57. doi: 10.13205/j.hjgc.202403006

Citation:

ZHOU Qi, HAN Peipei, HOU Yanan, HUANG Cong. CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(3): 51-57. doi: 10.13205/j.hjgc.202403006

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CHANGES IN MICROBIAL COMMUNITY OF NITRIFYING SLUDGE UNDER LONG-TERM CARBON DISULFIDE STRESS

doi: 10.13205/j.hjgc.202403006

1. Tianjin University of Science and Technology, Tianjin 300457, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China

Received Date: 2023-03-30
Available Online: 2024-05-31

Abstract

Abstract

About one million tons of CS₂ is used in the production of viscose fiber in China every year. CS₂ is often removed by aeration in industry. However, some CS₂ remains in the water and enters the nitrification unit of wastewater treatment, affecting operational performance. It was found that 10 mg/L CS₂ significantly inhibited nitrification sludge activity under short-term stress, low concentration of CS₂ (10 to 40 mg/L) could promote the metabolic activity and antioxidant activity of nitrification sludge, and high concentration of CS₂ (100 to 200 mg/L) could inhibit the activity. The nitrification reaction was completely inhibited under long-term CS₂ stress (20 to 200 mg/L), and white turbidity was found on the surface of nitrification sludge during long-term operation. The white turbidity was analyzed as simple sulfur by liquid chromatography and XPS, and the assimilation process of CS₂→COS→H₂S→S⁰→SO^2-₃→SO^2-₄ might occur. In 16S rRNA analysis, it was found that the abundance of Actinobacteria and Sinomonas involved in sulfur oxidation has increased, which also verified this hypothesis.
- carbon disulfide,
- nitrification,
- metabolism activity,
- oxidative stress,
- microbial community

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References(26)

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