Treatment of aniline wastewater by partial nitrificaion-denitrification IASBR process and microbial characteristics

WEI Fengqin; MENG Hailing; LIU Zailiang; Qiao Yakai; CAO Zupeng; TAO Shiyu

doi:10.13205/j.hjgc.202504016

Volume 43 Issue 4

Apr. 2025

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2025 > 43(4): 165-173

WEI F Q，MENG H L，LIU Z L，et al.Treatment of aniline wastewater by partial nitrificaion-denitrification IASBR process and microbial characteristics［J］.Environmental Engineering，2025，43（4）：165-173. doi: 10.13205/j.hjgc.202504016

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Treatment of aniline wastewater by partial nitrificaion-denitrification IASBR process and microbial characteristics

doi: 10.13205/j.hjgc.202504016

1. School of Energy and Environment, Anhui University of Technology, Ma'anshan 243032, China;
2. Ministry of Education Biofilm Process Water Purification and Utilization Technology Engineering Research Center, Anhui University of Technology, Ma'anshan 243032, China

Received Date: 2023-04-25
Accepted Date: 2023-06-18
Rev Recd Date: 2023-05-21
Publish Date: 2025-04-01

Abstract

Abstract

The study investigated the performance of an intermittent aeration sequencing batch reactors （IASBR） in low dissolved oxygen conditions （approximately 0.5 mg/L）. Degradation of organic compounds and nitrogen removal through the partial nitrification and denitrification were studied for treating of wastewater with different initial aniline concentrations （5， 20 and 30 mg/L）. The evolution of microbial communities were also analyzed based on high-throughput sequencing data. The experimental results indicated that when aniline concentration was from 5 mg/L to 30 mg/L， the IASBRs demonstrated excellent performance in removal of COD and aniline. The average removal rate of COD exceeded 80%. The removal rate of aniline was 85% above on average when the reactors gradually stabilized in the later stages. When the concentrations of aniline were 5 mg/L and 20 mg/L， the average NH₄⁺-N removal rates were 98.01% and 93.03%， the average total inorganic nitrogen removal rates were 83.79% and 79.74%， and the average accumulation rates of nitrite were 86.58% and 95.57%， respectively. The performance of partial nitrification and denitrification could effectively achieve nitrogen removal. However， when the aniline concentration increased to 30 mg/L， the average removal rates of NH₄⁺-N and total inorganic nitrogen decreased to 50.57% and 40.48%， respectively， because higher concentrations of aniline inhibited the activity of nitrobacteria and denitrifying bacteria. High-throughput sequencing results revealed a slight decrease in microbial diversity of sludge after adding aniline. The dominant phyla in test group C0 （inoculated sludge）， C1 （5 mg/L）， and C2 （20 mg/L） were Bacteroidetes， Proteobacteria and Candidatus_Saccharibacteria. With increasing influent aniline concentration， certain bacterial genera capable of degrading aromatic amine compounds were enriched， such as unclassified_Sphingobacteriales. However， bacterial genera involved in nitrogen metabolism were inhibited， such as unclassified_Xanthomonadaceae. Additionally， AOB （ammonia oxidizing bacteria） had a significant advantage over NOB （nitrite oxidizing bacteria） in three sludge samples， indicating that AOB had stronger tolerance to aniline. It was the main reason for achieving partial nitrification denitrification in the system. Metabolic predictions based on PICRUSt software and annotation analysis using the KEGG database indicated that the microbial metabolism of aniline in the system mainly involved ortho-metabolism and meta-metabolism processes.
- intermittent aeration,
- partial nitrification-denitrification,
- aniline,
- microbial community,
- metabolic pathway

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References(34)

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