Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure

JIANG Leyong; ZHAO Yingchu; SUN Shihao; XING Yiyuan; WANG Bo; PENG Yongzhen; ZHANG Lujing; PANG Hongtao; YANG Shenhua

doi:10.13205/j.hjgc.202509009

Volume 43 Issue 9

Sep. 2025

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2025 > 43(9): 72-81

JIANG Leyong, ZHAO Yingchu, SUN Shihao, XING Yiyuan, WANG Bo, PENG Yongzhen, ZHANG Lujing, PANG Hongtao, YANG Shenhua. Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(9): 72-81. doi: 10.13205/j.hjgc.202509009

Citation:

JIANG Leyong, ZHAO Yingchu, SUN Shihao, XING Yiyuan, WANG Bo, PENG Yongzhen, ZHANG Lujing, PANG Hongtao, YANG Shenhua. Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(9): 72-81. doi: 10.13205/j.hjgc.202509009

Citation:

JIANG Leyong, ZHAO Yingchu, SUN Shihao, XING Yiyuan, WANG Bo, PENG Yongzhen, ZHANG Lujing, PANG Hongtao, YANG Shenhua. Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(9): 72-81. doi: 10.13205/j.hjgc.202509009

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Effects of hydraulic retention time on biofilm attachment in moving bed biofilm reactors for aspects of biomass and microbial community structure

doi: 10.13205/j.hjgc.202509009

1. Xinkai Environment Investment Co., Ltd., Beijing 101101, China;
2. National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology, Beijing University of Technology, Beijing 100024, China;
3. Institute of Quantitative & Technological Economics, CASS, Beijing 100732, China

Received Date: 2024-11-26
Available Online: 2025-11-05
Publish Date: 2025-09-01

Abstract

Abstract

Formation process is a key step for a moving bed biofilm reactor （MBBR）， and the hydraulic retention time （HRT） is one of the key influencing factors. This study established four biofilm reactors， using raw water from a sewage treatment plant as the influent to explore the effects of different HRT strategies on biomass and microbial community structure of biofilm. Results revealed that the short HRT strategy （2 h） achieved a biomass of （21.23±1.2） mg SS/carrier in 15 d， which showed significant advantages compared with the three strategies： no water intake， long HRT strategy （6 h） and long-short HRT switching strategy （6 h → 2 h）. SEM and MiSeq Illumina analysis of the samples at 15 d， 32 d， and 57 d revealed that the microbial community structure of the biofilm changed significantly over time. Acinetobacter and Pseudomonas were the main genera with an increasing trend， while Ottowia and Acidovorax were the main genera with a decreasing trend. However， the H-test for α-diversity and the ANOSIM/Adonis test for β-diversity of the microbial community structure revealed no significant differences among the four groups under different HRT strategies. This indicates that different HRT strategies influence the biomass of the biofilm but have no significant effect on its microbial community structure. Based on these findings， this study proposes a functional biofilm initiation method described as "biofilm attachment first， domestication afterward".
- biofilm,
- biofilm attachment,
- pure biofilm reactor,
- MBBR

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References(35)

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