Short-term effect of hydrazine on operation and nitrogen removal performance of anammox biofilm

LOU Yuqing; WU Yixue; CHEN Song; YUAN Quan; ZHANG Yanping; SUN Yingxue

doi:10.13205/j.hjgc.202512009

Volume 43 Issue 12

Dec. 2025

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LOU Yuqing, WU Yixue, CHEN Song, YUAN Quan, ZHANG Yanping, SUN Yingxue. Short-term effect of hydrazine on operation and nitrogen removal performance of anammox biofilm[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(12): 73-80. doi: 10.13205/j.hjgc.202512009

Citation:

LOU Yuqing, WU Yixue, CHEN Song, YUAN Quan, ZHANG Yanping, SUN Yingxue. Short-term effect of hydrazine on operation and nitrogen removal performance of anammox biofilm[J]. ENVIRONMENTAL ENGINEERING , 2025, 43(12): 73-80. doi: 10.13205/j.hjgc.202512009

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Short-term effect of hydrazine on operation and nitrogen removal performance of anammox biofilm

doi: 10.13205/j.hjgc.202512009

School of Ecology and Environment, Beijing Technology and Business University, Beijing 100048, China

Received Date: 2023-05-15
Accepted Date: 2023-08-21
Rev Recd Date: 2023-07-14

Available Online: 2026-01-09

Abstract

Abstract

With the addition of hydrazine （N₂H₄）， Anammox’s low activity in municipal wastewater applications can be increased. To investigate the short-term effects of various N₂H₄ concentrations on the nitrogen removal capability of ANAMMOX biofilms， the change in matrix degradation rate， N₂H₄ degradation rate， the stoichiometric ratio of chemical reaction， extracellular polymer （EPS）， heme， and microbial community were analyzed. The results showed that the addition of N₂H₄ increased the consumption rate of NO^-₂-N and decreased the formation rate of NO^-₃-N. When the concentration of N₂H₄ was 20 mg/L， the highest total nitrogen removal efficiency （NRR）of 78.35 mg/（L·d） was achieved， which was three times higher than the control group. The analysis of EPS extracted from biomass showed that， the maximum concentration of TB-EPS was 10.55 mg/g when the concentration of N₂H₄was 20 mg/L， that was 0.9 times higher than the control， and the increase in TB-EPS concetration can inprove the stability of the biofilm； the highest concentration of heme was 3.00 μmol/g when the concentration of N₂H₄ was 20 mg/L. As a result， short-term addition of 20 to 40 mg/L of N₂H₄ can promote the growth of AnAOB and enchance the stability of the ANAMMOX biofilm system.
- Anammox,
- N₂H₄,
- biofilm,
- EPS,
- heme

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References(47)

References

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