Effects of rhamnolipids on growth， microcystin production and release of <i>Microcystis aeruginosa</i>

SHAO Chenxi; NI Lixiao; XU Chu; SANG Wenlu; ZHANG Yi; DU Cunhao; LI Shiyin

doi:10.13205/j.hjgc.202601007

Volume 44 Issue 1

Jan. 2026

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2026 > 44(1): 61-69

SHAO Chenxi, NI Lixiao, XU Chu, SANG Wenlu, ZHANG Yi, DU Cunhao, LI Shiyin. Effects of rhamnolipids on growth， microcystin production and release of Microcystis aeruginosa[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(1): 61-69. doi: 10.13205/j.hjgc.202601007

Citation:

SHAO Chenxi, NI Lixiao, XU Chu, SANG Wenlu, ZHANG Yi, DU Cunhao, LI Shiyin. Effects of rhamnolipids on growth， microcystin production and release of Microcystis aeruginosa[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(1): 61-69. doi: 10.13205/j.hjgc.202601007

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Effects of rhamnolipids on growth， microcystin production and release of Microcystis aeruginosa

doi: 10.13205/j.hjgc.202601007

SHAO Chenxi^1,2,
NI Lixiao^{1,2
,
,},
XU Chu^1,2,
SANG Wenlu^1,2,
ZHANG Yi^1,2,
DU Cunhao^1,2,
LI Shiyin³

1. College of Environment, Hohai University, Nanjing 210098, China;
2. Key Laboratory of Integrated Regulation and Resource Development on Shallow Lakes, Ministry of Education, Hohai University, Nanjing 210098, China;
3. College of Environment, Nanjing Normal University, Nanjing 210023, China

Received Date: 2024-07-10
Available Online: 2026-02-26
Publish Date: 2026-01-22

Abstract

Abstract

Rhamnolipid， a common biosurfactant， has an inhibitory effect on some marine microalgae. To investigate the effects of rhamnolipids on the growth and microcystin production of Microcystis aeruginosa and the impact mechanism， the growth， microcystin production and release， intracellular oxygen radicals， malondialdehyde content， and cell membrane morphology of Microcystis aeruginosa under rhamnolipid stress were measured. The results indicated that 8 mg/L rhamnolipid inhibited more than 90% of Microcystis aeruginosa at the beginning of the logarithmic growth period by day 10， and 95.5% by the end of the test， with significant inhibition of algal growth within 21 days. Rhamnolipids enhanced the MC-LR production capacity of the algal cells， with the mcyD gene up-regulated to 1.1 times that of the blank group， but the total amount of MC-LR produced by the algal cells was significantly reduced， and no significant difference in the amount of extracellular MC-LR released was observed. The overloading of superoxide anion in algal cells under rhamnolipid stress caused lipid peroxidation of cell membranes， decomposition of membrane lipids into malondialdehyde， disruption of membrane structure， and consequent release of intracellular MC-LR. The result can provide a theoretical basis for the practical application of rhamnolipids.
- rhamnolipid,
- Microcystis aeruginosa,
- microcystin,
- biosurfactant,
- superoxide anion

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References(34)

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