Diversity and structure of bacterial and archaeal communities in a carbonate-salt lake， Chali Co Lake in Tibet

LU Xinyue; CHANG Xiaoyan; TAO Yujie; HAN Rui; LI Yongzhen; ZHU Derui; WANG Rong

doi:10.13205/j.hjgc.202602011

Volume 44 Issue 2

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LU Xinyue, CHANG Xiaoyan, TAO Yujie, HAN Rui, LI Yongzhen, ZHU Derui, WANG Rong. Diversity and structure of bacterial and archaeal communities in a carbonate-salt lake， Chali Co Lake in Tibet[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(2): 92-102. doi: 10.13205/j.hjgc.202602011

Citation:

LU Xinyue, CHANG Xiaoyan, TAO Yujie, HAN Rui, LI Yongzhen, ZHU Derui, WANG Rong. Diversity and structure of bacterial and archaeal communities in a carbonate-salt lake， Chali Co Lake in Tibet[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(2): 92-102. doi: 10.13205/j.hjgc.202602011

Citation:

LU Xinyue, CHANG Xiaoyan, TAO Yujie, HAN Rui, LI Yongzhen, ZHU Derui, WANG Rong. Diversity and structure of bacterial and archaeal communities in a carbonate-salt lake， Chali Co Lake in Tibet[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(2): 92-102. doi: 10.13205/j.hjgc.202602011

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Diversity and structure of bacterial and archaeal communities in a carbonate-salt lake， Chali Co Lake in Tibet

doi: 10.13205/j.hjgc.202602011

1. Department of Basic Medical Sciences, Medical College, Qinghai University, Xining 810016, China;
2. Qinghai Provincial Key Laboratory of Vegetable Genetics and Physiology, Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, China

Received Date: 2025-03-12
Available Online: 2026-04-11
Publish Date: 2026-02-01

Abstract

Abstract

Chali Co Lake is a high-altitude （4650 m） carbonate-salt lake located in the Qinghai-Tibet Plateau. The community structure composition of bacteria and archaea involved is still unclear. This study employed high-throughput Illumina sequencing to analyze the diversity of bacteria and archaea in Chali Co Lake. By a 97% similarity level， operational taxonomic units （OTUs） were noted and analyzed based on the phylum， class， order， and genus level， respectively. The Redundancy analysis （RDA） was employed to examine the relationship between the dominant genus and environmental factors. A total of 37 phyla， 54 classes， 78 orders， and 417 genera （1，678 OTUs） of bacteria， and 10 phyla， 9 classes， 15 orders， and 32 genera （526 OTUs） of archaea were identified. The predominant bacterial genus were Pelobacter （4.60% to 9.58%）， Aquiflexum （1.08% to 13.97%）， Desulfurivibrio （0.64% to 8.07%） and Desulfocapsa （6.87%）； The dominant archaeota genera were Woesearchaeota AR16 （72.60% to 86.31%）、AR18 （2.71% to 6.45%） and AR15 （0.26% to 6.19%）. Significant correlation between the bacterial community structure and the concentrations of TS， HCO₃^-， and SO₄^2- （P<0.05）. Pelobacter exhibited a significant correlation with T， TN， and Na⁺ concentrations. Aquiflexum demonstrated a significant correlation with TOC， TN， and temperature. Additionally， both Desulfurivibrio and Desulfocapsa exhibited correlations with temperature， SO₄^2-， and HCO₃^- concentrations. The structure of archaeal communities was most strongly correlated with K⁺， TS， and pH. The dominant genera AR16 and AR18 subgroups of archaea exhibited a significant correlation with TP， Mg²⁺， and K⁺. The AR15 subgroup demonstrated a significant correlation with TOC， TN， and SO₄^2-. This study revealed the community structure of bacteria and archaea and their species diversity in Chali Co Lake， and provided a theoretical basis for developing halophilic bacteria and exploiting microbial resources.
- Chali Co Lake,
- carbonate-salt lake,
- bacteria,
- archaea,
- high-throughput sequencing,
- diversity

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References(56)

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