Source Jouranl of CSCD
Source Journal of Chinese Scientific and Technical Papers
Included as T2 Level in the High-Quality Science and Technology Journals in the Field of Environmental Science
Core Journal of RCCSE
Included in the CAS Content Collection
Included in the JST China
Indexed in World Journal Clout Index (WJCI) Report
Volume 44 Issue 7
Jul.  2026
Turn off MathJax
Article Contents
CHU Zhaorui, ZUO Jianing, XU Kaicheng, GUO Qingsong, HE Junguo. Research on enhancing sludge dewatering and filtrate carbon source recovery through typical bioenzymatic conditioning[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(7): 242-250. doi: 10.13205/j.hjgc.202607024
Citation: CHU Zhaorui, ZUO Jianing, XU Kaicheng, GUO Qingsong, HE Junguo. Research on enhancing sludge dewatering and filtrate carbon source recovery through typical bioenzymatic conditioning[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(7): 242-250. doi: 10.13205/j.hjgc.202607024

Research on enhancing sludge dewatering and filtrate carbon source recovery through typical bioenzymatic conditioning

doi: 10.13205/j.hjgc.202607024
  • Received Date: 2026-01-19
    Available Online: 2026-09-01
  • This study systematically investigated the effects of single and composite conditioning with lysozyme, amylase, and protease on sludge dewatering performance and carbon source recovery in filtrate from the residual sludge of a water treatment plant in Foshan City. The results indicated that lysozyme significantly improved sludge dewatering performance by reducing specific resistance of filtration (SRF), capillary suction time (CST), and the water content of the filtered sludge cake (Wc). While amylase and protease decreased Wc, and elevated SRF and CST,deteriorated sludge filtration properties. Among the combined enzyme treatments, lysozyme and protease exhibited synergistic effects. The asynchronous addition strategy (protease/amylase followed by lysozyme) demonstrated the best performance in reducing Wc, while simultaneous addition was more effective in improving SRF and CST. Mechanistic analysis revealed that all three enzymes reduced sludge particle size. Lysozyme primarily targeted cell lysis and wall disruption, releasing intracellular substances, reducing viscosity, and enhancing sludge hydrophobicity. Meanwhile, amylase and protease mainly disrupted the structure of extracellular polymeric substances (EPS), leading to the release of large amounts of proteins and polysaccharides into the slime (S)-EPS, thereby increasing viscosity and hydrophilicity. Furthermore, all three enzymes effectively promoted carbon source release, increased the soluble chemical oxygen demand (SCOD) of the filtrate, and transformed recalcitrant humic acid-like organic matters into readily bioavailable tryptophan-like and tyrosine-like substances.
  • loading
  • [1]
    JIANG Y,GAO F,ZHANG N,et al. Dehydration performance of municipal sludge and its dewatering conditioning methods:a review[J]. 2023,62(29):11337-11357.
    [2]
    LIU Z,LUO F,HE L,et al. Physical conditioning methods for sludge deep dewatering:A critical review[J]. Journal of Environmental Management,2024,360:121207.
    [3]
    LIN W,LIU X,DING A,et al. Advanced oxidation processes(AOPs)-based sludge conditioning for enhanced sludge dewatering and micropollutants removal:A critical review[J]. Journal of Water Process Engineering,2022,45:102468.
    [4]
    QI Y,CHEN J,XU H,et al. Optimizing sludge dewatering efficiency with ultrasonic treatment:insights into parameters,effects,and microstructural changes[J]. Ultrasonics Sonochemistry,2024,102:106736.
    [5]
    QI Y,WANG W,YANG Z,et al. Enhancing activated sludge dewatering performance:Impact of alum dosage on dehydration characteristics and microstructure[J]. Drying Technology,2024,42(15):2232-2240.
    [6]
    LI L,PENG C,ZHAN Z,et al. A novel treatment for amelioration of sludge dewaterability using green starch-grafted flocculant and realized mechanism[J]. Separation and Purification Technology,2022,301:122060.
    [7]
    LAN B,JIN R,LIU G,et al. Improving waste activated sludge dewaterability with sodium periodate pre-oxidation on extracellular polymeric substances[J]. Water Environment Research,2021,93(9):1680-1689.
    [8]
    CAO B,ZHANG T,ZHANG W,et al. Enhanced technology based for sewage sludge deep dewatering:A critical review[J]. Water Research,2021,189:116650.
    [9]
    TCHOBANOGLUS G,BURTON F,STENSEL H D. Wastewater engineering:treatment and reuse[J]. American Water Works Association Journal,2003,95(5):201.
    [10]
    PRIMO E D,OTERO L H,RUIZ F,et al. The disruptive effect of lysozyme on the bacterial cell wall explored by an in-silico structural outlook[J]. Biochemistry and Molecular Biology Education,2017,46(1):83-90.
    [11]
    WEI X F,MENG S T,WANG Y T,et al. Effects of replacing fish meal with biofloc meal on growth performance,nutrients metabolism,immune response and intestinal microbiota of common carp(Cyprinus carpio)[J]. Aquaculture,2024,591:741124.
    [12]
    PEI H Y,HU W R,LIU Q H. Effect of protease and cellulase on the characteristic of activated sludge[J]. Journal of Hazardous Materials,2010,178:397-403.
    [13]
    YANG S F,LI X Y. Influences of extracellular polymeric substances(EPS)on the characteristics of activated sludge under non-steady-state conditions[J]. Process Biochemistry,2009,44(1):91-96.
    [14]
    TAN X P,HE J G,KUANG K,et al. Characteristics of dissolved organic matter and denitrification potential in fermentation liquid of membrane bar screen screenings[J]. Environmental Science,2017,38(5):1986-1993. 谭小萍,赫俊国,匡科,等. 膜格栅截留物发酵液的溶解性有机物组分特性及其反硝化作用研究[J]. 环境科学,2017,38(5):1986-1993.
    [15]
    KOMATSU K,ONODERA T,KOHZU A,et al. Characterization of dissolved organic matter in wastewater during aerobic,anaerobic,and anoxic treatment processes by molecular size and fluorescence analyses[J]. Water Research,2020,171:115459.
    [16]
    COHEN E,LEVY G J,BORISOVER M. Fluorescent components of organic matter in wastewater:Efficacy and selectivity of the water treatment[J]. Water Research,2014,55:323-334.
    [17]
    BERTO S,de LAURENTIIS E,SCAPUZZI C,et al. Phototransformation of l-tryptophan and formation of humic substances in water[J]. Environmental Chemistry Letters,2018,16(3):1035-1041.
    [18]
    YAO M,LUO H Y,XIE X Q,et al. Three dimensional excitation-emission fluorescence spectra of exopolymer substances in activated sludge of WWTP[J]. China Environmental Science,2012,32(1):94-99. 姚萌,罗红元,谢小青,等. 城市污水厂活性污泥胞外聚合物的三维荧光特性分析[J]. 中国环境科学,2012,32(1):94-99.
    [19]
    ZHANG D,WANG Y,WANG J,et al. Rethinking the relationships between gel like structure and sludge dewaterability based on a binary gel like structure model:Implications for the online sensing of dewaterability[J]. Water Research,2024,249:120971.
  • 加载中

Catalog

    通讯作者: 陈斌, bchen63@163.com
    • 1. 

      沈阳化工大学材料科学与工程学院 沈阳 110142

    1. 本站搜索
    2. 百度学术搜索
    3. 万方数据库搜索
    4. CNKI搜索

    Article Metrics

    Article views (10) PDF downloads(0) Cited by()
    Proportional views
    Related

    /

    DownLoad:  Full-Size Img  PowerPoint
    Return
    Return