3D bioprinting <i>S. Oneidensis</i> MR-1 hydrogel enhances decolorization of azo dyes: performance and mechanism

GUO Weihao; HOU Yanan; LIU Zhihua; LI Haibo; HAN Yi; CHEN Zhi; HUANG Cong

doi:10.13205/j.hjgc.202609008

Volume 44 Issue 9

Sep. 2026

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Article Navigation > ENVIRONMENTAL ENGINEERING > 2026 > 44(9): 69-78

GUO Weihao, HOU Yanan, LIU Zhihua, LI Haibo, HAN Yi, CHEN Zhi, HUANG Cong. 3D bioprinting S. Oneidensis MR-1 hydrogel enhances decolorization of azo dyes: performance and mechanism[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(9): 69-78. doi: 10.13205/j.hjgc.202609008

Citation:

GUO Weihao, HOU Yanan, LIU Zhihua, LI Haibo, HAN Yi, CHEN Zhi, HUANG Cong. 3D bioprinting S. Oneidensis MR-1 hydrogel enhances decolorization of azo dyes: performance and mechanism[J]. ENVIRONMENTAL ENGINEERING , 2026, 44(9): 69-78. doi: 10.13205/j.hjgc.202609008

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3D bioprinting S. Oneidensis MR-1 hydrogel enhances decolorization of azo dyes: performance and mechanism

doi: 10.13205/j.hjgc.202609008

GUO Weihao^1,2,
HOU Yanan^{1,2
,},
LIU Zhihua¹,
LI Haibo¹,
HAN Yi¹,
CHEN Zhi^1,3,
HUANG Cong²

1. Tianjin Chengjian University, Tianjin 300384, China;
2. Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin 300308, China;
3. Concordia University, Montreal H3G1M8, Canada

Received Date: 2025-01-17
Available Online: 2026-11-05

Abstract

Abstract

3D bioprinting technology demonstrates significant potential in environmental science field， particularly in the construction of microbial active materials. This study successfully developed a biohydrogel loaded with Shewanella oneidensis MR-1 using extrusion-based bioprinting technology. The hydrogel enhances microbial metabolic activity and electron transfer efficiency by integrating adsorption and biological decolorization mechanisms， thereby improving the decolorization efficiency of azo dyes. Experimental results indicated that the three-dimensional porous network structure of the biohydrogel exhibits a superior decolorization rate compared to traditional bacterial suspensions. Further analysis of biomass and microbial metabolic activity reveals that the biohydrogel provides effective protection for the encapsulated bacteria， reducing the oxidative stress levels （ROS） in the system and significantly promoting biomass increase. Additionally， the content of cytochrome C in the hydrogel system increased by 1.31 times compared to the bacterial suspension， and the electron transfer system activity （ETSA） rose by 26.05%. These findings suggest that the protective effect of the hydrogel substantially enhances the electron transfer capacity within the system. This research， constructs a biohydrogel loaded with S. oneidensis MR-1 by bioprinting technology， which significantly improves the decolorization efficiency of azo dyes and reveals the synergistic mechanism between the hydrogel and the microbe， thus providing a novel strategy for the efficient degradation of water pollutants.
- 3D bioprinting,
- hydrogel,
- direct blue71,
- electron transfer,
- metabolic activity

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References(43)

References

[1]	SEOW T W，LIM C K，NOR M H M，et al. Review on wastewater treatment technologies［J］. Int J Appl Environ Sci，2016，11（1）:111-126.
[2]	ALITALESHI F，DAGHBANDAN A，PENDASHTEH A. Performance of rice husk biocarrier on ammonia nitrogen removal in the MBBR treating aquaculture wastewater using biological attached growth process:Performance and kinetic study［J］. Journal of Environmental Chemical Engineering，2024，12（1）:111446.
[3]	WU T，DING J，YANG S S，et al. A novel cross-flow honeycomb bionic carrier promotes simultaneous nitrification，denitrification and phosphorus removal in IFAS system:Performance，mechanism and keystone species［J］. Water Research，2022，225:119132.
[4]	LIU Y，WAN H，NIU J，et al. 3D printing for constructing biocarriers using sodium alginate/ε-poly-l-lysine ink:Enhancing microbial enrichment for efficient nitrogen removal in wastewater［J］. Science of the Total Environment，2024，950:175296.
[5]	ELKORO A，CASANOVA I. 3D printing of structured nanotitania catalysts:a novel binder-free and low-temperature chemical sintering method［J］. 3D Printing and Additive Manufacturing，2018，5（3）:220-226.
[6]	CAPEL A J，RIMINGTON R P，LEWIS M P，et al. 3D printing for chemical，pharmaceutical and biological applications［J］. Nature Reviews Chemistry，2018，2（12）:422-436.
[7]	TANG Y，VARYAMBATH A，DING Y，et al. Porous organic polymers for drug delivery:Hierarchical pore structures，variable morphologies，and biological properties［J］. Biomaterials Science，2022，10（19）:5369-5390.
[8]	ZHAO T，LIU Y，WU Y，et al. Controllable and biocompatible 3D bioprinting technology for microorganisms:fundamental，environmental applications and challenges［J］. Biotechnology Advances，2023:108243.
[9]	YUSOFF N H M，CHONG C H，WAN Y K，et al. Optimization strategies and emerging application of functionalized 3D-printed materials in water treatment:A review［J］. Journal of Water Process Engineering，2023，51:103410.
[10]	JAKUS A，GEISENDORFER N，LEWIS P，et al. 3D-printing porosity:a new approach to creating elevated porosity materials and structures［J］. Acta Biomaterialia，2018，72:94-109.
[11]	QIAN F，ZHU C，KNIPE J M，et al. Direct writing of tunable living inks for bioprocess intensification［J］. Nano Letters，2019，19（9）:5829-5835.
[12]	DATTA D，WEISS E L，WANGPRASEURT D，et al. Phenotypically complex living materials containing engineered cyanobacteria［J］. Nature Communications，2023，14（1）:
[13]	OH J J，AMMU S，VRIEND V D，et al. Growth，distribution，and photosynthesis of Chlamydomonas reinhardtii in 3D Hydrogels［J］. Advanced Materials，2023，36（2）:
[14]	LOURO R O，RUSCONI G，FONSECA B M，et al. Deciphering molecular factors that affect electron transfer at the cell surface of electroactive bacteria:the case of OmcA from Shewanella oneidensis MR-1［J］. Microorganisms，2022，11（1）:79.
[15]	WU M，XU Y，ZHAO C，et al. Efficient nitrate and Cr（VI）removal by denitrifier:The mechanism of S. oneidensis MR-1 promoting electron production，transportation and consumption［J］. Journal of Hazardous Materials，2024，469:133675.
[16]	JIA B，LIU T，WAN J，et al. Enhancing the extracellular electron transfer ability via polydopamine@ S. oneidensis MR-1 for Cr（VI）reduction［J］. Environmental Research，2023，217:114914.
[17]	SHI H，JIANG X，WEN X，et al. Enhanced azo dye reduction at semiconductor-microbe interface:The key role of semiconductor band structure［J］. Water Research，2024，248:120846.
[18]	MA J F，HOU Y N，GUO J，et al. Rational design of biogenic PdxAuy nanoparticles with enhanced catalytic performance for electrocatalysis and azo dyes degradation［J］. Environmental Research，2022，204:112086.
[19]	YANG F A，HOU Y N，CAO C，et al. Mechanistic insights into the response of electroactive biofilms to Cd²⁺ shock:bacterial viability and electron transfer behavior at the cellular and community levels［J］. Journal of Hazardous Materials，2023，459:132183.
[20]	YANG F A，HOU Y N，CAO C，et al. Electroactive properties of EABs in response to long-term exposure to polystyrene microplastics/nanoplastics and the underlying adaptive mechanisms［J］. Journal of Hazardous Materials，2024，465:133438.
[21]	CHELU M，MUSUC A M，POPA M，et al. Chitosan hydrogels for water purification applications［J］. Gels，2023，9（8）:664.
[22]	SHALLA A H，BHAT M A，YASEEN Z. Hydrogels for removal of recalcitrant organic dyes:A conceptual overview［J］. Journal of Environmental Chemical Engineering，2018，6（5）:5938-5949.
[23]	WANG J，GUO X. Rethinking of the intraparticle diffusion adsorption kinetics model:Interpretation，solving methods and applications［J］. Chemosphere，2022，309:136732.
[24]	ZHANG H，SHI Z，LIU X，et al. Insights into graphene oxide double-network macro-monothlic adsorbent through 3D printing:Mechanical properties and Cu²⁺ adsorption mechanism［J］. Separation and Purification Technology，2025，132140.
[25]	SILVA E C，SOARES V R，NORNBERG A B，et al. Recyclable 3D-printed composite hydrogel containing rice husk biochar for organic contaminants adsorption in tap water［J］. ACS Applied Polymer Materials，2023，5（10）:8415-8429.
[26]	KHAN S A，KHAN M，SUN W，et al. Optimization of Gelatin-based 3D hydrogel scaffold for effective removal of Methylene Blue and Alizarin Red S from aqueous solutions and food samples:kinetic and isotherm investigations［J］. Polymer Bulletin，2025，82（2）:479-500.
[27]	NG T S，NORMAN A，MOHD Y N H，et al. 3D printing and optimization of biocompatible and hydrophilic PEGDA‐HEMA lattice for enhanced RhB dye removal from aqueous solution［J］. International Journal of Polymer Science，2024，2024（1）:6633503.
[28]	RANJAN R，RAI R，NAIK K，et al. Scalable phosphorylated cellulose production with improved environmental sustainability，crosslinkability and processability using 3D bioprinting for dye remediation［J］. International Journal of Biological Macromolecules，2024，264:130577.
[29]	SHOJAEIARANI J，SHIRZADIFAR A，BAJWA D S. Robust and porous 3D-printed multifunctional hydrogels for efficient removal of cationic and anionic dyes from aqueous solution［J］. Microporous and Mesoporous Materials，2021，327:111382.
[30]	YUAN J，YI C，JIANG H，et al. Direct ink writing of hierarchically porous cellulose/alginate monolithic hydrogel as a highly effective adsorbent for environmental applications［J］. ACS Applied Polymer Materials，2021，3（2）:699-709.
[31]	NIE F H，WU D，ZOU C L，et al. The preparation of magnetic calcium alginate gel beads and their adsorption of methylene blue［J］. Journal of Safety and Environment，2022，22（1）:458-467. 聂发辉，吴道，邹成龙，等. 磁性海藻酸钙凝胶球的制备及其对亚甲基蓝的吸附［J］. 安全与环境学报，2022，22（1）:458-467.
[32]	WU Z，QU S G，FENG L X，et al. Adsorption performance and mechanism of sodium alginate/microcrystalline cellulose composite hydrogel for aqueous methyl orange and methylene blue［J］. Chemical Industry and Engineering Progress，2024，43（8）:4681-4693. 武哲，曲树光，冯练享，等. 海藻酸钠/微晶纤维素复合水凝胶对水中甲基橙和亚甲基蓝的吸附性能与机理［J］. 化工进展，2024，43（8）:4681-4693.
[33]	CHANG H，XIA H M，WANG N. Application of polyaniline hydrogel adsorbent in azo dye removal［J］. Chemical Engineer，2022，36（9）:44-49. 常华，夏浩铭，王宁. 聚苯胺水凝胶吸附剂在偶氮染料去除中的应用研究［J］. 化学工程师，2022，36（9）:44-49.
[34]	ZHANG M Y，DING X G，SU T，et al. Preparation，characterization and dye adsorption properties of dextran/PDA Composite Gel［J］. Contemporary Chemical Industry，2022，51（4）:770-773. 张梦颖，丁晓光，苏婷，等. 葡聚糖/PDA复合凝胶的制备、表征及其染料吸附性能研究［J］. 当代化工，2022，51（4）:770-773.
[35]	CHANG S，ZHANG X，WANG C，et al. Efficient adsorption of rhodamine B using synthesized Mg-Al hydrotalcite/sodium carboxymethylcellulose/sodium alginate hydrogel spheres:performance and mechanistic analysis［J］. Heliyon，2024，10（9）:
[36]	HUSSAIN S，SALMAN M，AL-AHMARY K M，et al. Synthesis of potential adsorbent for removal of malachite green dye using alginate hydrogel nanocomposites［J］. International Journal of Biological Macromolecules，2025，289:138816.
[37]	ALTALEB H A. Effective removal of hazardous cationic dye from polluted water using sulfonated copolymer hydrogel:Synthesis，nonlinear isotherm，and kinetics investigation［J］. Journal of Saudi Chemical Society，2024，28（3）:101852.
[38]	YAN L K，FUNG K Y，NG K M. Aerobic sludge granulation for simultaneous anaerobic decolorization and aerobic aromatic amines mineralization for azo dye wastewater treatment［J］. Environmental technology，2018，39（11）:1368-1375.
[39]	LI P，MULLER M，CHANG M W，et al. Encapsulation of autoinducer sensing reporter bacteria in reinforced alginate-based microbeads［J］. ACS applied materials& interfaces，2017，9（27）:22321-22331.
[40]	RAMAMURTHY K，PRIYA P S，MURUGAN R，et al. Hues of risk:investigating genotoxicity and environmental impacts of azo textile dyes［J］. Environmental Science and Pollution Research，2024:1-22.
[41]	WEN X，HOU Y N，GUO J，et al. Mechanistic insight into enhanced methyl orange degradation by Raoultella planticola/MoS₂ biohybrid:Implication for electron transfer and microbial metabolism［J］. Journal of Cleaner Production，2024，469:143201.
[42]	LI R，HOU Y-N，LI H，et al. Salinity responsive mechanisms of sulfur-based mixotrophic denitrification and ectoine induced tolerance enhancement［J］. Chemical Engineering Journal，2024，496:154266.
[43]	BERTINI I，CAVALLARO G，ROSATO A. Cytochrome C:occurrence and functions［J］. Chemical reviews，2006，106（1）:90-115.