RESEARCH ON GROUNDWATER NITRATE REDUCTION EFFICIENCY BASED ON METHANOTROPH AND FUNCTIONAL MICROORGANISMS

LIAO Xun; LI Yancheng; ZHANG Yuduo; YANG Qilin; LI Jiang

doi:10.13205/j.hjgc.202402013

Volume 42 Issue 2

Feb. 2024

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LIAO Xun, LI Yancheng, ZHANG Yuduo, YANG Qilin, LI Jiang. RESEARCH ON GROUNDWATER NITRATE REDUCTION EFFICIENCY BASED ON METHANOTROPH AND FUNCTIONAL MICROORGANISMS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(2): 113-120. doi: 10.13205/j.hjgc.202402013

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LIAO Xun, LI Yancheng, ZHANG Yuduo, YANG Qilin, LI Jiang. RESEARCH ON GROUNDWATER NITRATE REDUCTION EFFICIENCY BASED ON METHANOTROPH AND FUNCTIONAL MICROORGANISMS[J]. ENVIRONMENTAL ENGINEERING , 2024, 42(2): 113-120. doi: 10.13205/j.hjgc.202402013

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RESEARCH ON GROUNDWATER NITRATE REDUCTION EFFICIENCY BASED ON METHANOTROPH AND FUNCTIONAL MICROORGANISMS

doi: 10.13205/j.hjgc.202402013

LIAO Xun¹,
LI Yancheng^{1,2
,
,},
ZHANG Yuduo¹,
YANG Qilin¹,
LI Jiang^1,2

1. Key Laboratory of Karst Georesources and Environment, Ministry of Education, College of Resources and Environmental Engineering, Guizhou University, Guiyang 550025;
2. Guizhou Karst Environmental Ecosystems Observation and Research Station, Ministry of Education, Guiyang 550025, China

Received Date: 2022-09-24
Available Online: 2024-04-28

Abstract

Abstract

Pollutants in groundwater are complicated from many sources, and nitrate pollutants are one of them. In this study, a methanotroph-based biofilm reactor was established to find a remediation method with low consumption, high efficiency, and no secondary pollutants, by assaying nitrate reduction efficiency, functional microorganisms, and metabolic pathway.Results showed that the maximum nitrate removal rate reached 98.83% when nitrate nitrogen concentration in the influent was 10 mg/L. DOM component changes found that the dissolved organic matter primarily was a tryptophan-like protein. Macrogenomic analysis revealed that the dominant aerobic methanotrophs in this biofilm reactor were Methylocystis(5.25%) and Methylomonas(2.73%); the dominant anaerobic methanotrophs were Methylmirabilis(0.0016%), and Methanoperedens(0.0016%); and the dominant nitrate-reducing bacteria were mainly Lysobacter(9.72%), Opitutus(2.74%), and Hypomicrobium(2.01%). The KEGG database was annotated to identify microbial functions in the system, including 40 methane-metabolism-related functional genes and 19 nitrogen-metabolism-related functional genes, as well as 5 relatively complete methane metabolism pathways and 6 nitrogen metabolism pathways. Thus, methanogens could be proved with the capability of partial denitrification and good selectivity for nitrate. Relevant research results can provide technical support and a theoretical basis for the remediation of nitrate-contaminated groundwater.
- methane oxidizing bacteria,
- groundwater,
- nitrate,
- metagenomics,
- functional microorganism

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References(30)

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