ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM

GUO Zhi-chao; XU Xian-bao; XU Ting-ting; ZHAO Ai-hua; TAI Jun; LIU Ya-nan; XUE Gang; LI Xiang

doi:10.13205/j.hjgc.202109023

Volume 39 Issue 9

Jan. 2022

Turn off MathJax

Article Contents

Article Navigation > ENVIRONMENTAL ENGINEERING > 2021 > 39(9): 160-168

GUO Zhi-chao, XU Xian-bao, XU Ting-ting, ZHAO Ai-hua, TAI Jun, LIU Ya-nan, XUE Gang, LI Xiang. ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023

Citation:

GUO Zhi-chao, XU Xian-bao, XU Ting-ting, ZHAO Ai-hua, TAI Jun, LIU Ya-nan, XUE Gang, LI Xiang. ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023

Citation:

GUO Zhi-chao, XU Xian-bao, XU Ting-ting, ZHAO Ai-hua, TAI Jun, LIU Ya-nan, XUE Gang, LI Xiang. ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM[J]. ENVIRONMENTAL ENGINEERING , 2021, 39(9): 160-168. doi: 10.13205/j.hjgc.202109023

PDF( 2795 KB)

ANALYSIS ON FERMENTATION PATHWAY AND CAPROATE PRODUCTION FROM FOOD WASTE BY DIFFERENT INOCULUM

doi: 10.13205/j.hjgc.202109023

1. College of Environmental Science and Engineering, Donghua University, Shanghai 201620, China;
2. Shanghai Chengtou Group Co., Ltd, Shanghai 200020, China;
3. Shanghai Environmental Sanitation Engineering Design Institute Co., Ltd, Shanghai 200232, China

Received Date: 2020-10-26
Available Online: 2022-01-21

Abstract

Abstract

Waste activated sludge, granular sludge, distiller's yeast, and vinasse were inoculated in the food waste fermentation for organic acid production. Batch tests were conducted to compare the solubilization, hydrolysis, and acidification processes using four different inoculums during fermentation. Based on the batch test, we investigated the effect of different electron donors on chain elongation to produce caproate. When activated sludge was used as the inoculum of food waste fermentation, the production of L-lactic acid reached 22.2 g COD/L and its optical activity reached 82.3%. It was an effective way to ferment food waste to produce highly optically active L-lactic acid by inoculating activated sludge. Caproate at a concentration of 4.2 g COD/L was produced by inoculating granular sludge during fermentation, indicating the strong capacity of caproate production of granular sludge. When distiller's yeast and vinasse were used as the inoculum, high concentration of butyrate (21.6 g COD/L for distiller's yeast inoculum and 20.4 g COD/L for vinasse inoculum) was obtained, which was the key electron acceptor during chain elongation. The concentration of caproate reached 8.8 g COD/L by inoculating vinasse (lactic acid as the electron donor) and 10.4 g COD/L by inoculating distiller's yeast (ethanol as the electron donor). Microbial community analysis revealed that Streptococcus was the dominant genus, accounting for 80% and 55% by inoculating distiller's yeast and vinasse groups, respectively, showing a strong correlation with the production of high concentration caproate.
- food waste,
- inoculum,
- fermentation,
- carbon chain elongation,
- electron donor,
- caproate

FullText(HTML)

References(38)

References

[1]	ZHOU M M,YAN B H,WONG J W C,et al.Enhanced volatile fatty acids production from anaerobic fermentation of food waste:a mini-review focusing on acidogenic metabolic pathways[J].Bioresource Technology,2018,248:68-78.
[2]	夏旻,毕珠洁,张瑞娜,等.上海市餐厨垃圾理化特性及资源化预处理对策研究[J].环境卫生工程,2013,21(6):1-2.
[3]	YAN B H,SELVAM A,WONG J W C.Application of rumen microbes to enhance food waste hydrolysis in acidogenic leach-bed reactors[J].Bioresource Technology,2014,168:64-71.
[4]	SHEN F,YUAN H R,PANG Y Z,et al.Performances of anaerobic co-digestion of fruit & vegetable waste (FVW) and food waste (FW):single-phase vs.two-phase[J].Bioresource Technology,2013,144:80-85.
[5]	ABREU A A,TAVARES F,ALVES M M,et al.Garden and food waste co-fermentation for biohydrogen and biomethane production in a two-step hyperthermophilic-mesophilic process[J].Bioresource Technology,2019,278:180-186.
[6]	OLESKOWICZ-POPIEL P,KADAR Z,HEISKE S,et al.Co-production of ethanol,biogas,protein fodder and natural fertilizer in organic farming:evaluation of a concept for a farm-scale biorefinery[J].Bioresource Technology,2012,104:440-446.
[7]	FEI Q,FU R Z,SHANG L A,et al.Lipid production by microalgae Chlorella protothecoides with volatile fatty acids (VFAs) as carbon sources in heterotrophic cultivation and its economic assessment[J].Bioprocess and Biosystems Engineering,2015,38(4):691-700.
[8]	ZHENG J,GAO M,WANG Q H,et al.Enhancement of L-lactic acid production via synergism in open co-fermentation of Sophora flavescens residues and food waste[J].Bioresource Technology,2017,225:159-164.
[9]	WU Q L,BAO X,GUO W Q,et al.Medium chain carboxylic acids production from waste biomass:current advances and perspectives[J].Biotechnology Advances,2019,37(5):599-615.
[10]	ZHAO J W,ZHANG C,WANG D B,et al.Revealing the underlying mechanisms of how sodium chloride affects short-chain fatty acid production from the co-fermentation of waste activated sludge and food waste[J].Acs Sustainable Chemistry & Engineering,2016,4(9):4675-4684.
[11]	COOK S M,SKERLOS S J,RASKIN L,et al.A stability assessment tool for anaerobic co-digestion[J].Water Research,2017,112:19-28.
[12]	CHEN Y G,LUO J Y,YAN Y Y,et al.Enhanced production of short-chain fatty acid by co-fermentation of waste activated sludge and kitchen waste under alkaline conditions and its application to microbial fuel cells[J].Applied Energy,2013,102(S1):1197-1204.
[13]	WU S L,SUN J,CHEN X M,et al.Unveiling the mechanisms of medium-chain fatty acid production from waste activated sludge alkaline fermentation liquor through physiological,thermodynamic and metagenomic investigations[J].Water Research,2020,169:1-12.
[14]	ZHU X Y,TAO Y,LIANG C,et al.The synthesis of n-caproate from lactate:a new efficient process for medium-chain carboxylates production[J].Scientific Reports,2015,5:14360.
[15]	CARVAJAL-ARROYO J M,CANDRY P,ANDERSEN S J,et al.Granular fermentation enables high rate caproic acid production from solid-free thin stillage[J].Green Chemistry,2019,21(6):1330-1339.
[16]	YU J N,HUANG Z X,WU P,et al.Performance and microbial characterization of two-stage caproate fermentation from fruit and vegetable waste via anaerobic microbial consortia[J].Bioresource Technology,2019,284:398-405.
[17]	GILDEMYN S,MOLITOR B,USACK J G,et al.Upgrading syngas fermentation effluent using Clostridium kluyveri in a continuous fermentation[J].Biotechnology for Biofuels,2017,10:1-15.
[18]	TAO Y,ZHU X Y,WANG H,et al.Complete genome sequence of Ruminococcaceae bacterium CPB6:a newly isolated culture for efficient n-caproic acid production from lactate[J].Journal of Biotechnology,2017,259:91-94.
[19]	LOWRY O H,ROSEBROUGH N J,FARR A L,et al.Protein measurement with the Folin phenol reagent[J].The Journal of biological chemistry,1951,193(1):265-275.
[20]	GOEL R,MINO T,SATOH H,et al.Enzyme activities under anaerobic and aerobic conditions inactivated sludge sequencing batch reactor[J].Water Research,1998,32(7):2081-2088.
[21]	MU H,CHEN Y G.Long-term effect of ZnO nanoparticles on waste activated sludge anaerobic digestion[J].Water Research,2011,45(17):5612-5620.
[22]	XU X B,ZHANG W J,GU X,et al.Stabilizing lactate production through repeated batch fermentation of food waste and waste activated sludge[J].Bioresource Technology,2020,300:122709.
[23]	NYBROE O,JORGENSEN P E,HENZE M.Enzyme-activities in waste-water and activated-sludge[J].Water Research,1992,26(5):579-584.
[24]	ZHANG D P,SPADARO D,VALENTE S,et al.Cloning,characterization,expression and antifungal activity of an alkaline serine protease of Aureobasidium pullulans PL5 involved in the biological control of postharvest pathogens[J].International Journal of Food Microbiology,2012,153(3):453-464.
[25]	LIANG S,GLINIEWICZ K,MENDES-SOARES H,et al.Comparative analysis of microbial community of novel lactic acid fermentation inoculated with different undefined mixed cultures[J].Bioresource Technology,2015,179:268-274.
[26]	REDCORN R,ENGELBERTH A S.Identifying conditions to optimize lactic acid production from food waste co-digested with primary sludge[J].Biochemical Engineering Journal,2016,105:205-213.
[27]	ANGENENT L T,RICHTER H,BUCKEL W,et al.Chain elongation with reactor microbiomes:open-culture biotechnology to produce biochemicals[J].Environmental Science & Technology,2016,50(6):2796-2810.
[28]	ZHANG W J,LI X,ZHANG T,et al.High-rate lactic acid production from food waste and waste activated sludge via interactive control of pH adjustment and fermentation temperature[J].Chemical Engineering Journal,2017,328:197-206.
[29]	TASHIRO Y,KANEKO W,SUN Y Q,et al.Continuous D-lactic acid production by a novel thermotolerant Lactobacillus delbrueckii subsp lactis QU 41[J].Applied Microbiology And Biotechnology,2011,89(6):1741-1750.
[30]	BI D X,CHU D Q,ZHU P,et al.Utilization of dry distiller's grain and solubles as nutrient supplement in the simultaneous saccharification and ethanol fermentation at high solids loading of corn stover[J].Biotechnology Letters,2011,33(2):273-276.
[31]	COMA M,VILCHEZ-VARGAS R,ROUME H,et al.Product diversity linked to substrate usage in chain elongation by mixed-culture fermentation[J].Environmental Science & Technology,2016,50(12):6467-6476.
[32]	KUCEK L A,NGUYEN M,ANGENENT L T.Conversion of L-lactate into n-caproate by a continuously fed reactor microbiome[J].Water Research,2016,93:163-171.
[33]	TARASOV A L,BORZENKOV I A,BELYAYEV S S.Investigation of the trophic relations between anaerobic microorganisms from an underground gas repository during methanol utilization[J].Microbiology,2011,80(2):180-187.
[34]	CHEN Y,JIANG X,XIAO K,et al.Enhanced volatile fatty acids (VFAs) production in a thermophilic fermenter with stepwise pH increase:investigation on dissolved organic matter transformation and microbial community shift[J].Water Research,2017,112:261-268.
[35]	AGLER M T,SPIRITO C M,USACK J G,et al.Chain elongation with reactor microbiomes:upgrading dilute ethanol to medium-chain carboxylates[J].Energy & Environmental Science,2012,5(8):8189-8192.
[36]	KIM B C,SEUNG JEON B,KIM S,et al.Caproiciproducens galactitolivorans gen.nov.,sp.nov.,a bacterium capable of producing caproic acid from galactitol,isolated from a wastewater treatment plant[J].International Journal of Systematic and Evolutionary Microbiology,2015,65(12):4902-4908.
[37]	AGLER M T,WERNER J J,ITEN L B,et al.Shaping reactor microbiomes to produce the fuel precursorn-butyrate from pretreated cellulosic hydrolysates[J].Environmental Science & Technology,2012,46(18):10229-10238.
[38]	KHOR W C,ANDERSEN S,VERVAEREN H,et al.Electricity-assisted production of caproic acid from grass[J].Biotechnol Biofuels,2017,10(1):180-191.